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Adhesion, growth and differentiation of skin cells on nanofibrous polymer membranes
Pajorová, Júlia ; Bačáková, Lucie (advisor) ; Eckhardt, Adam (referee)
Our study contributes to the tissue engineering, mainly to the construction of appropriate scaffolds for regeneration of damaged skin. Simultaneously, it brings valuable insights for basic research in the field of molecular mechanisms of adhesion, proliferation and phenotypic maturation of cells and the control of the cell behavior through the cell extracellular matrix (ECM), represented by synthetic nanofibrous material. Nanofibrous polylactic-co-glycolic acid (PLGA) membranes were prepared by needle-less electrospinning technology. These membranes were further modified with cell adhesion-mediating biomolecules, e.g. collagen, fibronectin and fibrin in order to increase their affinity to colonizing cells. Adhesion, growth and differentiation of keratinocytes (HaCaT) and fibroblasts, i.e. major cell types of epidermis and dermis, were evaluated on these nanofibrous membranes. The results show that the membrane modification using fibrin structures improved adhesion and proliferation of human dermal fibroblasts. The collagen structure on the surface of membranes improved the adhesion and proliferation of human HaCaT keratinocytes. Furthermore, fibrin structure stimulated fibroblasts to produce collagen, which is a major component of ECM in the natural skin dermis. Fibronectin enhanced cell attachment...
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Methadone Maintenance Treatment - The pharmacokinetics and the pharmacodynamics as a basis.
Pajorová, Júlia ; Hejnová, Lucie (advisor) ; Melkes, Barbora (referee)
The aim of this study was to summarize the pharmacokinetic and pharmacodynamic knowledge about methadone, and to compare it with morphine on both molecular and clinical level. Methadone and morphine are μ opioid agonists, and therefore their effect on the organism is similar. However, the existence of fundamental differences between them, is decisive for their use in clinical practice. It was discovered that on a molecular level, methadone has a higher efficiency to internalise μ receptors than morphine, moreover it's potential to form addiction and tolerance is lower. From a pharmacokinetic point of view, methadone in contrary to morphine has a much longer elimination half-life, which brings a lots of benefits and lots of disadvantages. One of the benefits is longer effect, which together with the lower potential for tolerance and addiction development, predetermined methadone to be used for maintenance therapy for patients addicted to opiates. Maintenance therapy is based on the replacement of an illegal drug (mainly heroine), for an opiate of similar nature, which on the other hand has more favourable effects on the patient (methadone). The purpose of it is to ease the development of withdrawal symptoms during therapy's first stages, and with gradual lowering of methadone dosage, to lead the...
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