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DOES ONE MONTH CREATINE SUPPLEMENTATION IMPROVE BLOOD LIPIDS?
Kohlíková, E. ; Petr, M. ; Navrátil, Tomáš ; Heyrovský, Michael ; Přistoupilová, K. ; Přistoupil, T. I.
One month’s administration of creatine (CR) in the dose of 5 g/day to 11 young active sportsmen affected their daily amount of CR excreted into urine, and the levels of cholesterol (CH), of triacylglycerols (TAG), of vitamin B12 and of folates in blood. The probands were divided into 4 groups, according to the amount of CR found in urine, and of folates and vitamin B12 determined in blood. It is possible that added CR might induce individually higher offer of 2C units, released under catalysis of vitamin B12 from HoCySH and serine in enzymatic reaction catalyzed by CBS, and in this way higher offer of cysteine and succinyl-CoA This is the main effect of CR in the group D. Both vitamins influence the ratio between glycine (necessary mainly for CR synthesis, beside other metabolic pathways) and serine. The change of serine into glycine can be realized by THF, under catalysis of serine hydroxymethyltransferase. For the opposite change of glycine into serine the metabolic pathway is catalyzed by folates too, but in more complicated way
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Suplementace kreatinu u sportovce s hyperhomocysteinemií
Kohlíková, E. ; Petr, M. ; Navrátil, Tomáš ; Přistoupilová, K. ; Přistoupil, T. I. ; Pelclová, D. ; Žák, J. ; Heyrovský, Michael
One month's administration of creatine (CR) in the dose of 5 g/day to 11 young active sportsmen affected their daily amount of CR and creatinine excreted into urine. The probands were divided into 4 groups, according to the amount of CR found in urine, and of folates and of vitamin B12 determined in blood. The ganges of folates and vitamin B12 were mutually reciprocal. Each group utilized CR as donor of one- and two-carbon (1C and 2C) units by means of homocysteine (HoCySH), folates, and vitamin B12, in different metabolic pathways. In 10 men the CR administration was accompanied by an increase of HoCySH level in blood, while for the last man, with accidentally discovered hyperhomocysteinemia, after the month's CR administration the HoCySH level dropped by 50 %.
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Význam lipidů v metabolismu tuků
Kohlíková, E. ; Petr, M. ; Přistoupilová, K. ; Přistoupil, T. I. ; Pelclová, D. ; Žák, J. ; Heyrovský, Michael ; Navrátil, Tomáš
In course of last two years we compared values of 34 parameters, obtained from 11 young active sportsmen given 5 g of creatine (CR) daily during 1 month. Among these parameters were the products of metabolic pathways, the changes of which are connected with the development of atherosclerosis. This holds for the levels of cholesterol (CH), triacylglycerols (TAG) and homocysteine (HoCySH), and for the levels of vitamin B12 and of folates in blood.
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Vliv suprafyziologických dávek kreatinu na homocysteinový metabolismus u sportovců
Kohlíková, E. ; Petr, M. ; Přistoupilová, K. ; Šenholdová, Z. ; Přistoupil, T. I. ; Pelclová, D. ; Žák, J. ; Heyrovský, Michael ; Navrátil, Tomáš
One month´s administration of creatine (CR) in the dose of 5 g/day to 11 young men affected their daily amount of CR, creatinine, and thiodiglycolic acid (TDGA) excreted into urine. The probands were divided into 4 groups, according to the amount of CR found in urine, and of folates and of vitamin B12 determined in blood. The changes of folates and vitamin B12 were mutually reciprocal, as well as muscle mass and body fat. Each group utilized CR as donor of one- and two-carbon (1C and 2C) units by means of homocysteine (HoCySH), folates, and vitamin B12, in different metabolic pathways.
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Interaction of Creatine Metabolism with Three Possible Enzymatic Pathways Using Homocysteine
Přistoupilová, K. ; Navrátil, Tomáš ; Petr, M. ; Šenholdová, Z. ; Přistoupil, T. I. ; Pelclová, D. ; Žák, J. ; Kohlíková, E. ; Heyrovský, Michael
Alternative administration of creatine (CR) and vitamin B12 in food supplements has confirmed our finding that CR is a source of 2C units. They can be utilized in oxidative pathway, an intermediate of which is thiodiglycolic acid (TDGA). 2C units can be used also in other metabolic pathways, in which folates and vitamin B12 coenzymes take part together with homocysteine.
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Participation of electrochemistry in the studies of human metabolism
Navrátil, Tomáš ; Šenholdová, Z. ; Přistoupilová, K. ; Přistoupil, T. I. ; Heyrovský, Michael ; Pelclová, D. ; Žák, J.
The contribution deals with explanation of metabolism of long term supplemented creatine in human organism. This process differs from metabolism of natural endogenous creatine, which is excreted in form of creatinine. Exogenous creatine induces increase of peptide formation, of body mass, and of intracellular fluid.
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Vliv kreatinu aplikovaného jako potravinový doplněk na exkreci homocysteinu do krve a kyseliny thiodiglykolové do moře
Přistoupilová, K. ; Navrátil, Tomáš ; Šenholdová, Z. ; Přistoupil, T. I. ; Heyrovský, Michael ; Kohlíková, E. ; Petr, M. ; Pelclová, D.
Single-dose food supplementation of creatine (5 g of creatine per day), commonly used by sportsmen, affects their metabolism of thiocompounds and of some compounds in blood and in urine, the metabolism of which is connected with the synthesis of creatine or with its usage in the body. In blood the levels of folates, vitamin B12, homocysteine, triacylglycerols, total cholesterol, LDL cholesterol and HDL cholesterol, uric acid, cortizol, and testosterone were followed. In urine the changes in levels of creatine, creatinine, thiodiglycolic acid (TDGA), and pH were followed. The results were accomplished by data of bioimpedance measurements (body mass, muscle mass, body fat, intra- and extracellular fluids, total body water content, protein content, fitness score). It was found that according to the changes in creatine levels before and after administration of creatine in course of one month, it was possible to divide the subjects under study into four groups.
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Vliv kreatinu na koordinaci močovinového a citrátového cyklu
Kohlíková, E. ; Petr, M. ; Navrátil, Tomáš ; Šenholdová, Z. ; Přistoupilová, K. ; Přistoupil, T. I. ; Heyrovský, Michael ; Pelclová, D.
Creatine is recommended to be administered as food supplement, which should fortify its natural formation. It is supposed that human organism utilizes this compound for creatine phosphate formation, which is necessary for energy supplementation for muscular work. It was shown that the metabolic ways are more complicated than had been supposed. The changes in thiodiglycolic acid (TDGA) excretion into urine proved that application of creatine affects the metabolism of thiocompounds and two-carbon units, i.e., levels of homocysteine and glycine in blood, and of TDGA in urine. From our results, obtained in experiments with a group of 11 young men, who were supplemented by creatine for one month, it was confirmed that the dependence interrelationship among thiocompounds, vitamin B12, folates, cholesterol, and other complex is more complicated than had been supposed. For better understanding of the role of creatine in general metabolism, ...
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The use of electrochemistry for the study of human metabolism of sulfur-containing compounds
Navrátil, Tomáš ; Petr, M. ; Šenholdová, Z. ; Přistoupilová, K. ; Přistoupil, T. I. ; Heyrovský, Michael ; Pelclová, D. ; Kohlíková, E.
The contribution deals with explanation of metabolism of supplemented creatine in human organism. This process differs from metabolism of natural endogenous creatine, which is excreted in form of creatinine. Exogenous creatine induces increase of peptide formation, of body mass, and of intracellular fluid; from 4 to 6 hours after creatine supplementation the level of thiodiglycolic acid and pH in urine rapidly increase, both of them then decrease rapidly to the original values.
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Creatine fortification in relation to homocysteinemia
Kohlíková, E. ; Petr, M. ; Šenholdová, Z. ; Navrátil, Tomáš ; Heyrovský, Michael ; Přistoupilová, K. ; Přistoupil, T. I.
Some people supplement their diet by high doses of creatine (CR) to increase their muscle performace. In this way, they intensify the biochemical processes on cell membranes. The water thus formed is retained in the cells, which increases the body mass. The organism must remove exogenously added CR, probably in a similar way as in removing xenobiotics via oxidation with cyt.P450 to 2C units to cooperation with GSH. Our new simple and cheap voltammetric method enables to estimate thiogycolic (TDGA), the natural product of oxidative catabolism of thiocompounds, excreted to urine in regular catabolic processes.
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