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Model of cortical focal cerebral ischemia of a rat
Kaštylová, Klára ; Otáhal, Jakub (advisor) ; Brime, Tufikameni (referee)
Model of cortical focal cerebral ischemia of a rat. Aim: Determine the size of the damage of the cerebral cortex in the adult animal based on the length of irradiation a dose of Bengali red. Methods: An experimental study was conducted on specimens of an adult rat. Focal Cerebral ischemia was induced by the application of Bengali red. Assessment of damage cortex was done descriptively and with a special program has been calculated the size of the lesions which were then individually compared. The results: Demonstrate that the most appropriate parameter for comparison to measure the depth of the lesion lesion. Further shows that the lesion depth is dependent on the duration of irradiation, but not a direct linear relationship. And among the used concentrations of rose bengal were found significant differences. Key words: Focal cerebral ischemia, bengal rose, cerebral cortex, photochemical method Powered by TCPDF (www.tcpdf.org)
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The Occurence of Muscle Activity in Correlation with the EEG Recording of Developmentally Different Groups of Rats
Feštrová, Veronika ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
Objective: The aim of this thesis is to determine both the number and incidence of muscle twitches muscle activity in individual groups of pups in rats, possibly record the simultaneous occurrence of EEG activity, which points to the involvement of cortical levels of motor control. Method: The experiment was conducted on 4 groups of pups in rats, divided according to age, which was performed by the EMG electrodes into the extracellular space neck muscles and electrodes 6 - 4 sensor, reference and ground epidural placed for EEG activity. Have also been reported already mentioned curves and captured video footage necessary for final processing and analysis. Results: After statistical evaluation of experimental data has been demonstrated numerous of muscle jerks with developmentally youngest experimental group P7 and occurrence locomotor activity in developmentally older groups correlated with increased activation in cerebral cortex, which was confirmed by increased levels of energy and entropy of the EEG signal. These values also increased with increasing age, as evidenced by the gradual maturation of cortical motor control. Keywords: motor ontogenesis, surface electromyography, electroencephalography, descending path, rat, clonus Powered by TCPDF (www.tcpdf.org)
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Effect of background EMG activity on magnitude of evoked response
Trnková, Barbora ; Pánek, David (referee) ; Otáhal, Jakub (advisor)
Introduction: The purpose of this study is to investigate the effect of spontaneous electrical activity of a muscle on the degree of evoked response. Analysis of function of plantar flexor muscles and neuromuscular junction during indolent electric stimulation of fibular nerve in the poples may bring new information that might contribute to deepen our knowledge of biomechanical and neurophysiologic nature of changes of shape and latency of evoked reactions in various sorts of strain. The aim of the paper: To evoke H-reflex via electric stimulation of tibial nerve with escalating strain of plantar flexor muscles of the foot in supine position. To process and analyse data by computer. To assess findings of the study. Methods: The dissertation is made out in the form of processing of case data from volunteers, whose muscle electrical activity will be scanned via superficial electrodes attached to the skin above a muscle during indolent electrical stimulation of tibial nerve in the poples. The muscle will be strained using a weight and the muscle response evoked by the stimulation will be scanned using EMG. The measurement will be carried out in various sorts of strain (isometric-isotonic). The data will be further processed and analysed by computer. Results: The results clarify the H-reflex modulation during...
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The Role of Furosemide on Intrinsic Optical Signals in Hippocampal slices
Kuta, Igor ; Konopková, Renata (referee) ; Otáhal, Jakub (advisor)
Title of work: The Role of Furosemide on Intrinsic Optical Signals in hippocampal slices Objective: Learn how furosemide affects the internal optical signals in the rat hippocampus. Method: Measurement of IOS on slices of hippocampus in electro DG. detection IOS changes in the application of furosemide. Results: Internal optical signals are dependent on the activation of tissues and exhibit regional differences in hippocampal different layers. The concentration furosemide increases optical response of a tissue. Conclusion: The physiological processes in the nervous tissue are associated with changes in their optical properties. Furosemide in our work increased intrinsic optical signals. The change was more pronounced in dendritic compared with CA3 pyramidal layer. Keywords: hippocampus, an inner optical signals, furosemide, neuroglia, light transmission Powered by TCPDF (www.tcpdf.org)
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Damage of Neurons, Blood-brain Barrier and Folowing Immune Reaction During Ischemic Stroke After Endothelin-1 Application
Lutovská, Jana ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
Work objectives: The aim of this diploma work was taking up with microscopic identification metods of particular cell types of CNS, detection of blood-brain-barrier permeability and "dying" neurons. We are interested how the cellulate elements - neurons and astrocyte - are affected by cerebral ischemia and if the permeability of the blood-brain barrier is disrupted. Methodology: Experiments were performed in Wistar rats on postnatal day 12 or 25 (17 together). Cannula for vasoconstrictor Endothelin-1 infusion was inserted into left hemisphere to induce focal cerebral ischemia. Animals were let to live for 24 hours and then all rats were deeply anesthetized with urethane injection. The brains were sectioned in the coronal plane 50m thick. Adjacent series of sections were processed for Fluoro- Jade B to detect degenerating neurons, blood-brain barrier disruption was investigated using Evans Blue dye. Immunohistochemical labeling was used for IgG and visualization of hypertrofic astrocytes by GFAP. Common observation of brain was preformed under upride microscope with fluorescence. Results: Our findings demonstrate that focal cerebral ischemia generates induced by ET-1 into cerebral tissue damage. Degenerating neurons were observed in affected areas. Blood-brain barrier is disturbed in the same area as...
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Influence of temperature and stimulus intensity on FAD transience in rat hippocampal slices in vitro
Rous, Matouš ; Otáhal, Jakub (advisor) ; Konopková, Renata (referee)
Aims: The purpose of this study was to characterize the influence of temperature and stimulus intensity on flavoprotein autofluorescence in rat hippocampal slices. Methods: Experiments were performed in hippocampal slices of adult male Wistar rats. Slices of 400 µm of thickness were maintained in submerged recording chamber, through which oxygenated ACSF was perfused (2 ml/min). Synaptic activation was being made by bipolar stimulating electrode (10s stimulus train 20Hz) placed in hillus of the dentate gyrus (Mossy fibers). FAD signals were detected by cooled 12-bit CCD-camera (RETIGA2000R). Results: Our findings demonstrate that FAD autofluorescence signals were significantly decreased at higher temperature. The data confirmed that FAD autofluorescence signals showed smaller light intensity in different layers of CA3 under the same conditions at 36řC compared to 26řC. Our data approved that under defined conditions FAD fluorescence signals have risen during stimulation. Keywords: Mitochondria, FAD autofluorescence, temperature, stimulus intensity, hippocampus, rat.
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