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Implementation of the diagnosis of gastrointestinal nematodes using RT-PCR.
Bezdíčková, Kristýna ; Matoušková, Petra (advisor) ; Nguyen, Thuy Linh (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Kristýna Bezdíčková Supervisor: doc. Ing. Petra Matoušková, Ph.D. Title of diploma thesis: Implementation of the diagnosis of gastrointestinal nematodes using RT-PCR Gastrointestinal nematodes can cause various diseases in farm animals. Individuals are often infected with several types of parasites at once. Since the symptoms tend to be similar, the disease is called parasitic gastroenteritis. Not only do nematodes threaten the animal health, but they can also have a major impact on the economy of the entire farm. Diagnosis is not always easy. In addition to morphological and coprological methods, biochemical or molecular analysis can be used, which tends to be more sensitive and accurate. We tried to diagnose gastrointestinal nematodes using the molecular RT-PCR method. In the thesis we focused on 6 species of nematodes (Haemonchus contortus, Teladorsagia circumcinta, Trichostrongylus spp., Nematodirus battus, Chabertia ovina and Ashworthius sidemi). The material used was feaces samples from sheep from which we isolated gDNA. The control material was gDNA isolated from H. contortus eggs and gDNA obtained from whole parasite bodies. RT-PCR was tested using the fluorescent dye SYBR Green I and...
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Advances in the discovery and testing of anthelmintics
Nguyen, Thuy Linh ; Skálová, Lenka (advisor) ; Lamka, Jiří (referee) ; Várady, Marián (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training Workplace Department of Biochemical Sciences Doctoral Degree Program Xenobiochemistry and Pathobiochemistry Candidate Mgr. Thuy Linh Nguyen Supervisor Prof. RNDr. Lenka Skálová, Ph.D. Advisor Assoc. Prof. Ing. Petra Matoušková, Ph.D. Title of Doctoral Thesis Advances in the discovery and testing of anthelmintics The parasitic nematodes cause a considerable problem in human and animal health worldwide. The group of gastrointestinal nematodes is responsible for economic losses in livestock production. The treatment relies on the use of a limited number of anthelmintics, but their efficacy is hampered due to widespread drug resistance. Given the need for new drugs, the present thesis focuses on discovering novel compounds with potential anthelmintic effects and on the development of testing methods. Based on the literature search, we presented the possible approaches in the current development of new anthelmintics and evaluated their advantages and disadvantages in a review article. For the experiments, we used a model organism Haemonchus contortus, which is one of the most important gastrointestinal pathogens of small ruminants. The primary phenotypic screening of a small compound library against larvae identified two 'hit' compounds...
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Advances in the discovery and testing of anthelmintics
Nguyen, Thuy Linh ; Skálová, Lenka (advisor) ; Lamka, Jiří (referee) ; Várady, Marián (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Training Workplace Department of Biochemical Sciences Doctoral Degree Program Xenobiochemistry and Pathobiochemistry Candidate Mgr. Thuy Linh Nguyen Supervisor Prof. RNDr. Lenka Skálová, Ph.D. Advisor Assoc. Prof. Ing. Petra Matoušková, Ph.D. Title of Doctoral Thesis Advances in the discovery and testing of anthelmintics The parasitic nematodes cause a considerable problem in human and animal health worldwide. The group of gastrointestinal nematodes is responsible for economic losses in livestock production. The treatment relies on the use of a limited number of anthelmintics, but their efficacy is hampered due to widespread drug resistance. Given the need for new drugs, the present thesis focuses on discovering novel compounds with potential anthelmintic effects and on the development of testing methods. Based on the literature search, we presented the possible approaches in the current development of new anthelmintics and evaluated their advantages and disadvantages in a review article. For the experiments, we used a model organism Haemonchus contortus, which is one of the most important gastrointestinal pathogens of small ruminants. The primary phenotypic screening of a small compound library against larvae identified two 'hit' compounds...
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The effect of humulene, caryophyllene and caryophylllene oxide on the selected hepatic biotransformation enzymes
Nguyen, Thuy Linh ; Skálová, Lenka (advisor) ; Szotáková, Barbora (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Thuy Linh Nguyen Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of humulene, caryophyllene and caryophyllene oxide on the activity of selected liver biotransformation enzymes Sesquiterpenes, secondary metabolites of higher plants, are formed from three isoprenoid units. They possess interesting biological activities, e.g. antibacterial, antimalarial or anticarcinogenic activities, however they could also influence the activity and/or the expression of biotransformation enzymes. The aim of this diploma thesis was to evaluate the effect of α-humulene (HUM), β-caryophyllene (CAR) and β-caryophyllene oxide (CAO) on the activity and expression of enzymes of phase I and II of xenobiotics biotransformation. In the first study, the potential inhibitory effect of HUM, CAO and CAR on the activity of selected enzymes in subcellular fractions obtained from rat liver homogenates was studied. In the second study, HUM, CAO and CAR were incubated with primary cultures of rat hepatocytes for 24 hours to determine the impact of sesquiterpenes on the viability of hepatocytes and potential modulatory effects of HUM, CAO, CAR on the activity and expression of selected...
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Accounting system in Vietnam
Nguyen, Thuy Linh ; Žárová, Marcela (advisor)
This thesis focuses on the accounting system in Vietnam.The beginning of thesis briefly summarizes the evolution of accounting in Vietnam. It further deals with the accounting regulation and it mainly by the general standard No. 1, the accounting law, the specific accounting legislation for companies. The end of thesis is a study about auditing on Vietnam. The work pays attention to current requirements to obtain a certificate for operate the accounting and auditing services in Vietnam.
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New trends in marketing communication
Nguyen, Thuy Linh ; Pešek, Ondřej (advisor) ; Zamazalová, Marcela (referee)
In today's business environment of sharp competition it is very difficult to gain favour of customers, as their needs are more difficult to satisfy as time progresses. This situation creates incentives for more efficient use of traditional as well as new communication media for establishing contacts with customers. The aim of this thesis is to analyze some of the current trends in area of marketing communication, elaborate on the grounds these trends are based upon, the reasons for their development and their weaknesses and strengths. A more detailed analysis will be dedicated to mobile marketing, which is one of the new, dynamically developing trends that has recently been showing increased popularity.
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