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Determination of clotrimazol and benzylalcohol in vaginal cream by means of HPLC
Chmurová, Klára ; Kastner, Petr (advisor) ; Mokrý, Milan (referee)
HPLC determination of clotrimazol and benzyl alcohol in vaginal cream Academic dissertation Klára Chmurová Charles University in Prague, Pharmaceutical Faculty in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control The task was to find out a method of simultaneous determination of clotrimazol and benzylalcohol in a vaginal cream. We were looking for proper modification of the pharmaceutical preparation (vaginal cream), then optimization of chromatographic conditions and validation of this HPLC method, too. The best results were obtained with DISCOVERY® HS F5, 5 µm, 4,5 × 150 mm together with the pre-column DISCOVERY® HS C18, 5 µm, 2,1 × 20 mm, mixture of acetonitril and buffer (K2HPO4 0,05mol/l, pH = 6,1 set up by 85% H3PO4) 60 : 40 (v/v) was used as the mobile phase, flow rate was 1 ml/min. Detection was realized by UV detector with wave length 223 nm. Time of analysis was shorter than 10 min.
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HPLC EVALUATION OG FUMAGILLIN IN THE MEDICINE
Strýčková, Kristýna ; Mokrý, Milan (advisor) ; Sochor, Jaroslav (referee)
HPLC EVALUATION OG FUMAGILLIN IN THE MEDICINE Rigorous thesis Mgr. Kristýna Strýčková Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control In this rigorous thesis is described analytic evaluation of fumagillin by using HPCL and stated the amount of fumagillin in the medicine. The measurment was implemented on chromatographic colony in size 3 x 150 mm I.D. with the Seladon SGX NH2 filling, 7μm. There was used the mobile phase composed of methanol: amonium acteate (aqueous solution 0,005 mol/l, v/v )in the ratio of 60:40, at the flow rate of 1ml/min and pressure of 2,5-2,6 MPa. The detection was performed at 335 nm using the UV detektor. The samples were spraeyd in cubage 20 μ/l. The international standard method was drawn up for determination of fumagillin in medicine. The most suitable sample from the selected conditions was nimesulid. Concerning validation parameters were verified linearity, accuracy, robustness, selection and detector limit. Further there was examined the stability in conditions of room temperature and the temperature od 2-8˚C. It was determined 0,0238 g of fumagillin in 1 g of medicine that is stated as 95,2% of neccessary amount.
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