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Stability of phytocanabinoids during storage of Cannabis sativa
Myšíková, Jitka ; Jezberová, Michaela (advisor) ; Mokrý, Milan (referee)
(English version) Title: Stability of phytocanabinoids during storage of Cannabis sativa Author: Mgr. Jitka Myšíková Supervisor: RNDr. Michaela Hamerníková, Ph.D. The rigorous work deals with the problems of determination of ∆9 -THC, CBD and CBN and their precursors. It assesses the influence of storage time and way on marijuana and on n-hexan extract of marijuana. A validation of the method of ∆9 -THC, CBD and CBN determination was carried out through HPLC with UV detection at wave length of 220 nm, mobile phase methanol : water ( 85 : 15), colomn SUPELCOSILTM LC-18-DB. The main aim was to detect the influence of various storage ways and storage time on principal content items of marijuana. There were 2 samples of marijuana available, each of them was divided in 12 parts, whereas 6 parts were placed in a plastic bag and the other 6 in a linen bag. After that one part of the sample in the plastic bag and one in the linen bag were located in 6 places, respectively from each sample. The places of storage were choosen with respect to possible location by marijuana users or to their possible experiments with this drug. The observation went on for 8,5 months. The samples were put in a refrigerator, an ice-box, a room with daylight, on an exterior window sill, under a UV lamp or a sample was soaked and...
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Determination of clotrimazol and benzylalcohol in vaginal cream by means of HPLC
Chmurová, Klára ; Kastner, Petr (advisor) ; Mokrý, Milan (referee)
HPLC determination of clotrimazol and benzyl alcohol in vaginal cream Academic dissertation Klára Chmurová Charles University in Prague, Pharmaceutical Faculty in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control The task was to find out a method of simultaneous determination of clotrimazol and benzylalcohol in a vaginal cream. We were looking for proper modification of the pharmaceutical preparation (vaginal cream), then optimization of chromatographic conditions and validation of this HPLC method, too. The best results were obtained with DISCOVERY® HS F5, 5 µm, 4,5 × 150 mm together with the pre-column DISCOVERY® HS C18, 5 µm, 2,1 × 20 mm, mixture of acetonitril and buffer (K2HPO4 0,05mol/l, pH = 6,1 set up by 85% H3PO4) 60 : 40 (v/v) was used as the mobile phase, flow rate was 1 ml/min. Detection was realized by UV detector with wave length 223 nm. Time of analysis was shorter than 10 min.
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HPLC EVALUATION OG FUMAGILLIN IN THE MEDICINE
Strýčková, Kristýna ; Mokrý, Milan (advisor) ; Sochor, Jaroslav (referee)
HPLC EVALUATION OG FUMAGILLIN IN THE MEDICINE Rigorous thesis Mgr. Kristýna Strýčková Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Chemistry and Drug Control In this rigorous thesis is described analytic evaluation of fumagillin by using HPCL and stated the amount of fumagillin in the medicine. The measurment was implemented on chromatographic colony in size 3 x 150 mm I.D. with the Seladon SGX NH2 filling, 7μm. There was used the mobile phase composed of methanol: amonium acteate (aqueous solution 0,005 mol/l, v/v )in the ratio of 60:40, at the flow rate of 1ml/min and pressure of 2,5-2,6 MPa. The detection was performed at 335 nm using the UV detektor. The samples were spraeyd in cubage 20 μ/l. The international standard method was drawn up for determination of fumagillin in medicine. The most suitable sample from the selected conditions was nimesulid. Concerning validation parameters were verified linearity, accuracy, robustness, selection and detector limit. Further there was examined the stability in conditions of room temperature and the temperature od 2-8˚C. It was determined 0,0238 g of fumagillin in 1 g of medicine that is stated as 95,2% of neccessary amount.
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