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Analysis of historical pharmaceutical preparation containing chicory from the 18th century
Lener, Tomáš ; Nesměrák, Karel (advisor) ; Kubíčková, Anna (referee)
A historical pharmaceutical preparation - chicory extract - from the 18th century whose authenticity was investigated was qualitatively analyzed using HPLC with UV/VIS and MS2 detection. A current dry chicory root was used as a source of selected chicory chemotaxonomic markers and an indicator of the success of extraction with different solvents. The authenticity of the historical extract was proved by a water solubility test. A VIS spectrometric determination of inulin was used and the inuline content was determined to be 52.4% in the current dry chicory root and 7.45% in the historical extract. The detection wavelength 254 and 320 nm was selected from measured UV/VIS spectra of selected standards and the literature. The authenticity of the historical chicory extract was proven by tandem mass spectrometry, and, moreover, arteincultone - a new chicory chemotaxonomic marker - was identified. Key words: HPLC-MS, HPLC-UV/VIS, UV/VIS Spectrometry, Chicory, Historical extract, Chicory root, Arteincultone
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