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Epigenetic regulation of gene expression in cancer progression and HIV-1 latency
Kovářová, Denisa ; Hejnar, Jiří (advisor) ; Černá, Marie (referee) ; Rösel, Daniel (referee)
Epigenetic mechanisms of transcriptional regulation and modulation of gene expression using RNA interference have become a powerfull tool for studying various cellular mechanisms, including tumor progression and viral latency. Advances in expression profiling have provided technology to detect candidate genes implicated in this complex process and bring other possibilities in the diagnosis and treatment of tumor diseases. In this work, I compare the gene expression profiles of v-src-transformed metastatic and nonmetastatic cells using microarray chip technology. Transcription factor homeodomain only protein X (HOPX) was identified as one of the differentially expressed genes. Activity of HOPX gene in several cancer types is usually controlled by promoter methylation. The role HOPX in metastatic formation was assessed by inoculation of cells with modulated expression of HOPX in a syngeneic chicken model system. After HOPX knockdown using shRNA, originally metastatic line showed decreased in vivo metastatic capacity. Further genomic analyses identified a cadre of genes affected by HOPX knockdown. These data demonstrate that HOPX is a metastasis-associated gene and that its knockdown decreases the metastatic activity of v-src-transformed cells through altered gene expression patterns. In the second...
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Alterations of the oncogenic properties of tumor cell lines by modulating oncogene expression. Example of v-src transformed chicken cell lines
Kovářová, Denisa ; Hejnar, Jiří (advisor) ; Španielová, Hana (referee)
4 Alterations of the oncogenic properties of tumor cell lines by modulating oncogene expression. Example of v-src transformed chicken cell lines Abstract v-Src protein tyrosine kinase is the product of the transforming gene transduced by avian Rous sarcoma virus. In contrast to proto-oncogene c-src, v-src lacks the negative-regulatory C-terminal domain and consequently shows a higher level of activity and transforming ability. In addition, v-Src contains point mutations throughout its coding region that probably contribute to the high level of intrinsic kinase activity. Long terminal repeats (LTR) comprise strong promoter-enhancer sequences and ensure efficient expression of the v-src gene. v-Src protein has a strong transforming potential in vitro and induces tumor development and growth in vivo. Moreover, it is implicated in metastatic formation. In several cancer types the elevated c-Src kinase activity caused pleiotropic cellular responses inducing transformation and metastasis. The aim of this diploma work was to reveal the role of v-Src in mediating tumor and metastatic progression in chicken cell lines PR9692 and PR9692-E9. Despite the low propensity of the chicken cells to immortalization, comparatively high immoratlization efficiency was observed in cells from ex vivo tumours growing progressively...
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Posouzení směsných krmných dávek (TMR) pro dojnice na separátoru krmiv
Kovářová, Denisa
This diploma thesis presents the effect of a diet on milk yield and composition in dairy cows. The aim is to provide a comprehensive view on the whole issue, which means considering the forage quality, the way total mixed rations (TMR) are prepared and the way feed is utilised in cows. More than one source of information must have been used to evaluate all these factors objectively. They include milk-performance-testing data, TMR chemical analyses, separating the rations on Penn State Particle Separator and evaluating cow manure on Digestion Analyzer.
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Alterations of the oncogenic properties of tumor cell lines by modulating oncogene expression. Example of v-src transformed chicken cell lines
Kovářová, Denisa ; Hejnar, Jiří (advisor) ; Španielová, Hana (referee)
4 Alterations of the oncogenic properties of tumor cell lines by modulating oncogene expression. Example of v-src transformed chicken cell lines Abstract v-Src protein tyrosine kinase is the product of the transforming gene transduced by avian Rous sarcoma virus. In contrast to proto-oncogene c-src, v-src lacks the negative-regulatory C-terminal domain and consequently shows a higher level of activity and transforming ability. In addition, v-Src contains point mutations throughout its coding region that probably contribute to the high level of intrinsic kinase activity. Long terminal repeats (LTR) comprise strong promoter-enhancer sequences and ensure efficient expression of the v-src gene. v-Src protein has a strong transforming potential in vitro and induces tumor development and growth in vivo. Moreover, it is implicated in metastatic formation. In several cancer types the elevated c-Src kinase activity caused pleiotropic cellular responses inducing transformation and metastasis. The aim of this diploma work was to reveal the role of v-Src in mediating tumor and metastatic progression in chicken cell lines PR9692 and PR9692-E9. Despite the low propensity of the chicken cells to immortalization, comparatively high immoratlization efficiency was observed in cells from ex vivo tumours growing progressively...
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