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The Project Proposal of New Information System Implementation
Kopečný, Michal ; Smolíková, Lenka (referee) ; Doskočil, Radek (advisor)
The focus of bachelor thesis is creating project proposal of new information systém using methods of project management. The first part is focused on explaining different methods, which will be used later in thesis. The second part deals with analysis of internal and external environment of company První brněnská strojírna Velká Bíteš a.s. Third part is where all the knowledge gathred in first and second part of thesis is applied onto the project itself.
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The Project Proposal of New Information System Implementation
Kopečný, Michal ; Smolíková, Lenka (referee) ; Doskočil, Radek (advisor)
The focus of bachelor thesis is creating project proposal of new information systém using methods of project management. The first part is focused on explaining different methods, which will be used later in thesis. The second part deals with analysis of internal and external environment of company První brněnská strojírna Velká Bíteš a.s. Third part is where all the knowledge gathred in first and second part of thesis is applied onto the project itself.
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The use of laser microdissection for the genotyping of biological material isolated during an abortion.
Beranová, Karina ; Krátký, Martin (advisor) ; Kopečný, Michal (referee)
The use of laser microdissection for the genotyping of biological material isolated during an abortion Abstract In forensic genetics laser microdissection is used mainly as a method of cell separation from samples of mixed biological traits of sexually motivated crimes. It can also be used in paternity testing via genotyping of biological material isolated during an abortion. The foetal part of placenta is separated from the acquired samples and a pure DNA profile of the foetus is determited. This DNA can be compared to the suspect's DNA. The goal of this project is the application of laser microdissection to separate chorionic villi from a placenta and the subsequent genetic analysis leading to the determination of a pure foetal DNA profile. A secondary goal was the preparation and optimization of the method necessary for its introduction to forensic practice. In the first phase the optimization of the sample preparation was conducted on test sections of muscular tissue and epithelial cells from a buccal swap. Experimental material acquired during abortions of voluntary donors was used in the second phase. This technique was simultaneously tested on material directly linked to criminal acts. The separation of 10 chorionic villi cells with the method of laser microdissection eventually resulted in the...
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Laser microdissection in forensic genetics: genetic analysis of sexual abuse evidence
Faltýsková, Zuzana ; Krátký, Martin (advisor) ; Kopečný, Michal (referee)
Laser microdissection is a novel technique of cell separation under microscopic view utilizing a laser beam. It has been used for cell separation from mixed samples in forensic genetics, such as vaginal smear in cases of sexual assault. In a rape investigation, few cells are usually recovered as the only evidence available for the assailant identification. When analysing a resistant cell type such as sperms, an effective and reliable method is required. This study aims to optimise the genetic analysis of laser microdissected sperms and to take the optimal protocol into practice. The goal is to develop a technique capable of recovery of reliable genotyping results from just a few cells. Genetic analysis was optimised for sperms separated from pure semen samples by laser microdissection. After optimisation, it was validated for sperms recovered from a vaginal smear. In the resulting genetic profiles some artifacts occured, that have been reported in samples with low DNA content. Possible solutions of these artifacts occurrence and elimination are discussed. In conclusion, the developement of an efficient and reliable genetic analysis method was successful: 10 sperms were recovered from vaginal smear and the genotyping result was capable of individual identification of the sperm donor. Powered by TCPDF...
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The use of laser microdissection for the genotyping of biological material isolated during an abortion.
Beranová, Karina ; Krátký, Martin (advisor) ; Kopečný, Michal (referee)
The use of laser microdissection for the genotyping of biological material isolated during an abortion Abstract In forensic genetics laser microdissection is used mainly as a method of cell separation from samples of mixed biological traits of sexually motivated crimes. It can also be used in paternity testing via genotyping of biological material isolated during an abortion. The foetal part of placenta is separated from the acquired samples and a pure DNA profile of the foetus is determited. This DNA can be compared to the suspect's DNA. The goal of this project is the application of laser microdissection to separate chorionic villi from a placenta and the subsequent genetic analysis leading to the determination of a pure foetal DNA profile. A secondary goal was the preparation and optimization of the method necessary for its introduction to forensic practice. In the first phase the optimization of the sample preparation was conducted on test sections of muscular tissue and epithelial cells from a buccal swap. Experimental material acquired during abortions of voluntary donors was used in the second phase. This technique was simultaneously tested on material directly linked to criminal acts. The separation of 10 chorionic villi cells with the method of laser microdissection eventually resulted in the...
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Laser microdissection in forensic genetics: genetic analysis of sexual abuse evidence
Faltýsková, Zuzana ; Kopečný, Michal (referee) ; Krátký, Martin (advisor)
Laser microdissection is a novel technique of cell separation under microscopic view utilizing a laser beam. It has been used for cell separation from mixed samples in forensic genetics, such as vaginal smear in cases of sexual assault. In a rape investigation, few cells are usually recovered as the only evidence available for the assailant identification. When analysing a resistant cell type such as sperms, an effective and reliable method is required. This study aims to optimise the genetic analysis of laser microdissected sperms and to take the optimal protocol into practice. The goal is to develop a technique capable of recovery of reliable genotyping results from just a few cells. Genetic analysis was optimised for sperms separated from pure semen samples by laser microdissection. After optimisation, it was validated for sperms recovered from a vaginal smear. In the resulting genetic profiles some artifacts occured, that have been reported in samples with low DNA content. Possible solutions of these artifacts occurrence and elimination are discussed. In conclusion, the developement of an efficient and reliable genetic analysis method was successful: 10 sperms were recovered from vaginal smear and the genotyping result was capable of individual identification of the sperm donor. Powered by TCPDF...
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