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The effect of trans-nerolidol and valencene in ovarian cancer cells
Zajdlová, Martina ; Skálová, Lenka (advisor) ; Králová, Věra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Martina Zajdlová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of trans-nerolidol and valencene in ovarian cancer cells Trans-nerolidol and valencene belong to sesquiterpenes, which present a large group of secondary plant metabolites. In practice, we can encounter them in the food or perfume industry. However, they are also used in traditional Chinese medicine. Recently, the biological activities of sesquiterpenes, which include e.g. anti-inflammatory or antineoplastic activities, were studied. Doxorubicin is one of the most important cytostatic drugs and various ways to improve its effectiveness and reduce side effects are being studied. One such usage is in combination therapy with natural substances, e.g. with sesquiterpenes. The aim of this study was to test antiproliferative effects of doxorubicin, trans-nerolidol and valencene on ovarian cancer cell lines SK-OV-3 and A2780. We also assayed the possible pro-oxidant potential of doxorubicin and terpenes by measuring the production of ROS. In addition, we evaluated the interaction between doxorubicin and terpenes by quantification of combination indexes. We also studied the possible effect of...
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Surface-modified nanoparticles in the treatment of serious CNS diseases
Mareková, Dana ; Jendelová, Pavla (advisor) ; Králová, Věra (referee) ; Jirák, Daniel (referee)
Glioblastoma multiforme (GBM) is a primary adult brain tumour with an unfavourable prognosis. Standard therapies have a number of side effects, posing a challenge and leading to attempts at new therapeutic approaches. One of these is the use of theranostic nanoparticles with modified surfaces. We have established primary lines from GBM patient samples and characterized them not only in 2D and 3D cultures but also after passaging in the brains of immunodeficient mice. We tested our modified superparamagnetic nanoparticles carrying the drug doxorubicin and RGDS peptides targeting tumor cells on these lines. The γ- Fe2O3@P(HP-MAH)-RGDS-Dox nanoparticles not only reduced cell proliferation in vitro but also incorporated into the tumor and inhibited tumor growth in vivo. We also developed magnetic nanoparticles containing Mn and Zn, which can replace commercially available iron oxide-based contrast agents with their magnetic properties and can be used to label cells for in vivo magnetic resonance imaging. Both in vitro and in vivo tests of the new nanoparticles have revealed that they can be used as a suitable and effective tool for cell tracking using magnetic resonance imaging even where the use of current contrast agents is contraindicated.
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The effect of trans-nerolidol and valencene in ovarian cancer cells
Zajdlová, Martina ; Skálová, Lenka (advisor) ; Králová, Věra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Martina Zajdlová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of trans-nerolidol and valencene in ovarian cancer cells Trans-nerolidol and valencene belong to sesquiterpenes, which present a large group of secondary plant metabolites. In practice, we can encounter them in the food or perfume industry. However, they are also used in traditional Chinese medicine. Recently, the biological activities of sesquiterpenes, which include e.g. anti-inflammatory or antineoplastic activities, were studied. Doxorubicin is one of the most important cytostatic drugs and various ways to improve its effectiveness and reduce side effects are being studied. One such usage is in combination therapy with natural substances, e.g. with sesquiterpenes. The aim of this study was to test antiproliferative effects of doxorubicin, trans-nerolidol and valencene on ovarian cancer cell lines SK-OV-3 and A2780. We also assayed the possible pro-oxidant potential of doxorubicin and terpenes by measuring the production of ROS. In addition, we evaluated the interaction between doxorubicin and terpenes by quantification of combination indexes. We also studied the possible effect of...
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The effect of humulene and caryophyllene oxide on the efficacy of 5-fluorouracil in colon cancer cells
Šadibolová, Michaela ; Skálová, Lenka (advisor) ; Králová, Věra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Michaela Šadibolová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of humulene and caryophyllene oxide on the efficacy of 5-fluorouracil in colon cancer cells 5-Fluorouracil (5-FU) represents a standard chemotherapeutic agent used in the treatment of several types of cancer diseases including the treatment of colorectal cancer. However, the clinical use of 5-FU is limited due to frequent chemoresistance of tumour cells. Sesquiterpenes are plant secondary metabolites that form a major part of plant essential oils. They have been found to possess a considerable antitumour activity and the ability to enhance the efficacy of some traditional chemotherapeutic agents. The aim of this study was to find out whether sesquiterpenes α-humulene (HUM) and caryophyllene oxide (CAO) influence the antiproliferative effect of 5-FU in human colorectal adenocarcinoma cell lines Caco-2 and SW-620 and to test the effect of 5-FU, CAO and their combination on the activity of initiator caspases 8 and 9 and effector caspase 3/7 in Caco-2 cell line. CAO showed strong antiproliferative effect in both cancer cell lines whereas HUM showed minimal effect on cell proliferation. In...
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Recruitment in a Firm
Maredová, Barbora ; Lochmanová, Veronika (advisor) ; Králová, Věra (referee)
The bachelor thesis deals with human resources in the company, especially with personnel activities and recruitment. The aim is to describe the processes of recruiting and selecting of workers in the global organization and after that to propose provision for improvement. The theoretical part is about the characteristics of the concept of human resources management, mainly focused on the professional acquisition and selection of workers. The practical part deals with information about these process, which are compared with theoretical knowledge. At the end of the thesis are proposed provision for the selected company.
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The effect of trans-nerolidol and valencene in ovarian cancer cells
Zajdlová, Martina ; Skálová, Lenka (advisor) ; Králová, Věra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Martina Zajdlová Supervisor: Prof. RNDr. Lenka Skálová, Ph.D. Title of diploma thesis: The effect of trans-nerolidol and valencene in ovarian cancer cells Trans-nerolidol and valencene belong to sesquiterpenes, which present a large group of secondary plant metabolites. In practice, we can encounter them in the food or perfume industry. However, they are also used in traditional Chinese medicine. Recently, the biological activities of sesquiterpenes, which include e.g. anti-inflammatory or antineoplastic activities, were studied. Doxorubicin is one of the most important cytostatic drugs and various ways to improve its effectiveness and reduce side effects are being studied. One such usage is in combination therapy with natural substances, e.g. with sesquiterpenes. The aim of this study was to test antiproliferative effects of doxorubicin, trans-nerolidol and valencene on ovarian cancer cell lines SK-OV-3 and A2780. We also assayed the possible pro-oxidant potential of doxorubicin and terpenes by measuring the production of ROS. In addition, we evaluated the interaction between doxorubicin and terpenes by quantification of combination indexes. We also studied the possible effect of...
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Účinky seleničitanu sodného na buňky lidského kolorektálního karcinomu s odlišným p53 genotypem
Králová, Věra ; Rudolf, Emil (advisor) ; Skálová, Lenka (referee) ; Hofmanová, Jiřina (referee)
Effects of sodium selenite in human colon cancer cells with different p53 status Colorectal carcinoma (CRC) is one of the leading causes of cancer - associated mortality. Great effort is spent on the investigation of prevention possibilities by various types of environmental factors. Several epidemiological studies and clinical studies have shown an inverse association between selenium intake and the risk of different types cancer including CRC. Also in animal models and cell lines in vitro selenium compounds have shown ability to inhibit proliferation and induce cell death. The aim of this study was to assess the antiproliferative effects of sodium selenite in colorectal cancer cells in vitro, particularly in two model colorectal cancer cell lines HCT 116 differing in their p53 status. We have shown that sodium selenite in concentration range of 2.5 µM-10 µM inhibited proliferation and induced cell death in HCT 116 cells in a time- and concentration- dependent manner. Sodium selenite blocked the progression through the cell cycle in the S/G2-M phases, induced superoxide production in mitochondria, DNA damage and expression of p53 protein and activated cell death with involvement of caspases. Sodium selenite also increased the expression of autophagy markers. The HCT 116-p53KO cells, lacking...
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Motivational potential of the work and its use in the creation of incentive program
Filipová, Simona ; Hiršová, Miloslava (advisor) ; Králová, Věra (referee)
Thesis deals with motivation potential of work in a selected organisation, identification of presence of job characteristics according to Hackman and Oldham Job Characteristics Model, their perception of intensity and importace for employee. Furthermore proposing improvements of job characteristics and enrichment of employees' job. Another goal is to find satisfaction with current motivation program and in case of deficiencies to propose changes. Data were obtained by using questionary, this data were processing by descriptive statistics. In conclusion, were proposed possibles changes in case of job characteristics or in case of motivation program if enrichment of job characteristics was not suffice.
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Draft Standardization Project to re-mold Processes and Procedures in Oresi, Ltd
Broukalová, Martina ; Králová, Věra (advisor) ; Pirožek, Petr (referee)
The subject of this work was the now completed project pertaining to the reconstruction of kitchen work tops DOLTI made by Oresi s.r.o.. The aim here was to determine whether this project is standardizable or completely unique. Based on thorough research of the term project, i have created a concise list of characteristics of the term Research into the term standardization yelded the final choice of the PMI standard. Results of research into the term project allowed me to then identify individual sources of information. The focus was on qualitative data and where higher degree of understanding is required, an opinion from an expert of the relevant field is avaliable. Based on the analysis of the data gathered, it is then determined whether the reconstruction of DOLTI is a unique project and a form of standardization of repeated ations/tasks in accordance to the PMI standard is proposed to ensure potentional simplification and organisation of planning pertaining to future projects undertaken by Oresi s.ro.
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