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Spectral Properties of Benzimidazoles
Lukešová, Marie ; Jezberová, Michaela (referee) ; Kubíček, Vladimír (advisor)
Spectral Properties of Benzimidazoles Marie Lukešová Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Abstract: This diploma thesis deals with the spectral properties of benzimidazoles. Most of benzimidazoles are anthelmintic active drug, used primarily in veterinary medicine. Some substances are used as fungicides. The main aim of this study was to clarify the influence of solvents on the absorption spectra and especially on the fluorescence of benzimidazoles. The influence of aprotic and protic solvent, the influence of polarity of solvent and the influence of pH of solvents were studied. The thesis includes two parts: theoretical and practical. In the theoretical part physicochemical properties of benzimidazoles, benzimidazoles possibility of detection, UV-VIS and fluorescence spectrophotometry are summarized. In the practical part results of the measurement are processed into tables and graphs. It was found that the influence of solvent depends on the structure of the substance. Therefore it is necessary to check each substance separately.
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Stability of phytocanabinoids during storage of Cannabis sativa
Myšíková, Jitka ; Jezberová, Michaela (advisor) ; Mokrý, Milan (referee)
(English version) Title: Stability of phytocanabinoids during storage of Cannabis sativa Author: Mgr. Jitka Myšíková Supervisor: RNDr. Michaela Hamerníková, Ph.D. The rigorous work deals with the problems of determination of ∆9 -THC, CBD and CBN and their precursors. It assesses the influence of storage time and way on marijuana and on n-hexan extract of marijuana. A validation of the method of ∆9 -THC, CBD and CBN determination was carried out through HPLC with UV detection at wave length of 220 nm, mobile phase methanol : water ( 85 : 15), colomn SUPELCOSILTM LC-18-DB. The main aim was to detect the influence of various storage ways and storage time on principal content items of marijuana. There were 2 samples of marijuana available, each of them was divided in 12 parts, whereas 6 parts were placed in a plastic bag and the other 6 in a linen bag. After that one part of the sample in the plastic bag and one in the linen bag were located in 6 places, respectively from each sample. The places of storage were choosen with respect to possible location by marijuana users or to their possible experiments with this drug. The observation went on for 8,5 months. The samples were put in a refrigerator, an ice-box, a room with daylight, on an exterior window sill, under a UV lamp or a sample was soaked and...
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Determination of 6-sulfatoxymelatonin by capillary electrophoresis
Klimánková, Karla ; Jezberová, Michaela (advisor) ; Polášek, Miroslav (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biophysics and Physical Chemistry Candidate Mgr. Karla Klimánková Consultant RNDr. Michaela Hamerníková, Ph.D. Title of Thesis Determination of 6-sulfatoxymelatonine by Capillary Electrophoresis The submited thesis deals with a development of a method for determination of 6-sulfatoxymelatonine for purposes of the gerontological research. Parameters of the electrophoretic determination were optimized, i. e.: composition of the base electrolyte, its pH, voltage, time of injection. Then folloving validation parameters were determined: linearity, repeatability, reproducibility of migration times and limit of detection. The obtained results confirm the applicability of the method for the given purpose.
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