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National Repository of Grey Literature

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Metabolism and transport of 6-prenylnaringenin in vitro Horní, Martin ; Boušová, Iva (advisor) ; Raisová Stuchlíková, Lucie (referee) Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Martin Horni Supervisor: doc. PharmDr. Boušová Iva, Ph.D. Consultant: Mgr. Michaela Šadibolová Title of diploma thesis: Metabolism and transport of 6-prenylnaringenin in vitro Prenylflavonoids constitute a group of plant secondary metabolites with various positive effects on human health. Hops (Humulus lupulus L.) represent a rich source of prenylflavonoids including 6-prenylnaringenin (6-PN). Recently, 6-PN has been found to possess various biological effects including analgesic, antiproliferative, and estrogen-modulating activity. Despite its potential to be used as a nutraceutical, the pharmacokinetic properties of 6-PN are still unknown. This thesis had therefore two objectives. The first objective was to determine the hepatic and intestinal metabolism of 6-PN in humans as well as its hepatic metabolism in rats and mice in vitro using precision-cut tissue slices (PCTS). Samples were analyzed using UHPLC-MS/MS. 6-PN was efficiently metabolized predominantly into phase II metabolites with four glucuronides and four sulfates identified in the studied PCTS. For the first time, a novel metabolite of 6-PN formed as a result of concurrent glucuronidation and sulfation has been identified, which... Detailed record

Fluid mechanics and flow of body fluids Horní, Martin ; Kuchařová, Monika (advisor) ; Kubíček, Vladimír (referee) Detailed record

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