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The influence of the dominant trees and grain substrate the composition of the microbial community studied by PLFA
Stachová, Sandra ; Frouz, Jan (advisor) ; Heděnec, Petr (referee)
The aim of the thesis was to analyze soil microbial communities of three ecologically different locations, about 25 years old, forestry reclaimed dumps in the Republic of Poland, namely dumps of brown coal mine Bełchatów and sand mines Piaseczno and Szczakowa. I evaluated the degree of dependence of structure and composition of these communities on various substrate grain sizes and the influence of the dominant tree species. These were stands of birch (Betula pendula), pine (Pinus silvestris), oak (Quercus robur) and alder (Alnus glutinosa). Analysis of soil microbial communities was made by evaluating specific phospholipid fatty acids (PLFA) of microorganisms. It is the most appropriate way to implement the relatively rapid analysis of large numbers of samples, since PLFA are easily extractable and act as biomarkers indicating the presence of a number of different microorganisms (fungi, G- and G + bacteria, Actinobacteria, etc.) and thus allowing a qualitative and quantitative assessment of whole microbial communities. PLFA analysis enables to detect a total concentration of PLFA only in living soil microbial biomass. I analyzed 66 soil samples, 33 from an Oe layer and 33 from an A layer, every in three replications, i.e. three replications were collected at each location below each tree...
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Interactions between plants and soil biota and effect of energetic crops on these interactions
Heděnec, Petr ; Frouz, Jan (advisor) ; Pergl, Jan (referee) ; Mortimer, Simon (referee)
My PhD thesis is focused on study of plant-soil interactions. Chapter 1 is focused on general introduction to the interactions between plants and soil biota in terms of relationships between soil organisms and aboveground biomass as well as root biomass. Chapter 2 is focused on food interaction of Folsomia candida and soil microscopic fungi (Penicillium chrysogenum, Penicillium expansum, Absidia glauca, and Cladosporium herbarum). Fungi were grown separately on Petri dishes or on different litter type (oak, alder and willow) separately as well. Laboratory experiments showed that food preference was more influenced by different litter types than fungal species. In chapter 3 was studied long-term production of hybrid sorrel and its effect on the composition of the soil meso and macrofauna, basal soil respiration, microbial biomass and composition of cultivable fraction of soil microscopic fungi in comparison with oilseed rape and cultural meadow. There was found that long-term production of hybrid sorrel affects the composition of soil fauna, while the microbial activity of the soil was more affected by agriculture practices, especially tillage. In chapter 4 was studied the effect of native (Salix viminialis and Phalaris arudinacea) and introduced (Reynoutria sachalinensis, Silphium perfoliatum and...
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Final report about research in Sokolov post mining sites in 2020 based on agreement between ENKI ops and Institute of Soil Biology BC CAS
Frouz, Jan ; Bartuška, Martin ; Lišková, K. ; Angst, Gerrit ; Sun, Daquan ; Kučera, J. ; Bednářová, E. ; Spurná, V. ; Jílková, Veronika ; Heděnec, P. ; Ćápová, K. ; Müllerová, V. ; Irshach, S.
The report brings main results of the research about soil and ecosystem recovery in post mining landscape near Sokolov based on agreement between ENKI ops and Institute of soil biology, BC CAS.
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Final report about research in Sokolov post mining sites in 2019 based on agreement between ENKI ops and Institute of Soil Biology BC CAS
Frouz, Jan ; Bartuška, Martin ; Kopecký, T. ; Kučera, J. ; Bednářová, E. ; Krůček, M. ; Král, K. ; Kotápišová, M. ; Moradi, J. ; Lin, Qiang ; Dini-Andreote, F. ; Meador, Travis Blake ; Angel, Roey ; Harantová, L. ; Heděnec, P. ; Li, Lingjuan ; Baldrian, P. ; Angst, Šárka
The report brings main results of the research about soil and ecosystem recovery in post mining landscape near Sokolov based on agreement between ENKI ops and Institute of soil biology, BC CAS.
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The influence of the dominant trees and grain substrate the composition of the microbial community studied by PLFA
Stachová, Sandra ; Frouz, Jan (advisor) ; Heděnec, Petr (referee)
The aim of the thesis was to analyze soil microbial communities of three ecologically different locations, about 25 years old, forestry reclaimed dumps in the Republic of Poland, namely dumps of brown coal mine Bełchatów and sand mines Piaseczno and Szczakowa. I evaluated the degree of dependence of structure and composition of these communities on various substrate grain sizes and the influence of the dominant tree species. These were stands of birch (Betula pendula), pine (Pinus silvestris), oak (Quercus robur) and alder (Alnus glutinosa). Analysis of soil microbial communities was made by evaluating specific phospholipid fatty acids (PLFA) of microorganisms. It is the most appropriate way to implement the relatively rapid analysis of large numbers of samples, since PLFA are easily extractable and act as biomarkers indicating the presence of a number of different microorganisms (fungi, G- and G + bacteria, Actinobacteria, etc.) and thus allowing a qualitative and quantitative assessment of whole microbial communities. PLFA analysis enables to detect a total concentration of PLFA only in living soil microbial biomass. I analyzed 66 soil samples, 33 from an Oe layer and 33 from an A layer, every in three replications, i.e. three replications were collected at each location below each tree...
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Interactions between plants and soil biota and effect of energetic crops on these interactions
Heděnec, Petr ; Frouz, Jan (advisor) ; Pergl, Jan (referee) ; Mortimer, Simon (referee)
My PhD thesis is focused on study of plant-soil interactions. Chapter 1 is focused on general introduction to the interactions between plants and soil biota in terms of relationships between soil organisms and aboveground biomass as well as root biomass. Chapter 2 is focused on food interaction of Folsomia candida and soil microscopic fungi (Penicillium chrysogenum, Penicillium expansum, Absidia glauca, and Cladosporium herbarum). Fungi were grown separately on Petri dishes or on different litter type (oak, alder and willow) separately as well. Laboratory experiments showed that food preference was more influenced by different litter types than fungal species. In chapter 3 was studied long-term production of hybrid sorrel and its effect on the composition of the soil meso and macrofauna, basal soil respiration, microbial biomass and composition of cultivable fraction of soil microscopic fungi in comparison with oilseed rape and cultural meadow. There was found that long-term production of hybrid sorrel affects the composition of soil fauna, while the microbial activity of the soil was more affected by agriculture practices, especially tillage. In chapter 4 was studied the effect of native (Salix viminialis and Phalaris arudinacea) and introduced (Reynoutria sachalinensis, Silphium perfoliatum and...
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