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Proteome and Metabolome Changes in Selected Organisms under Stress
Halienová, Andrea ; Kráčmar, Stanislav (referee) ; Čertík, Milan (referee) ; Šucman, Emanuel (referee) ; Márová, Ivana (advisor)
Living conditions of every organism are influenced by various factors at this time. Some of them have positive effect on organism, some negative. Basic condition for surviving is the ability to resist and adapt to changing metabolic and living conditions. Every single stress effect can lead to changes in metabolism but organisms have ability to develope sufficient mechanisms for stress response. Some of them are similar for all living organisms (enzyme production, endogenous primary stress metabolites) some of them are specific for certain organism or stress type. Cell stress response can be observed on different levels (proteomic, genomic, metabolomic). In proper conditions it can be used indrustrially. In this work, influences of various stress factors were studied. These factors were applied on selected organisms – carotenogenic yeast and plant materials. Yeast stress response was induced by osmotic and oxidation stress factors. Changes on proteomic level and in production of selected secondary metabolites were observed. Proteome was analyzed by 1D and 2D electrophoresis with subsequent analysis of proteins by mass spectrometry. Yeast strain Rhodotorula glutinis CCY 20-2-26 showed the best adaptation to stress factors, which was moreover accompanied by overproduction of carotenoids. This finding can be premise for next industrial production of carotenoids. In plant samples predominantly enzymes and metabolites involved in antioxidant response were studied.
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Changes of the content of some active substances in aplle and pear fruits during storage
Matějková, Markéta ; Halienová, Andrea (referee) ; Márová, Ivana (advisor)
In presented bachelor thesis low molecular substances antioxidants found in plant food were described. Antioxidants from apples and pears were studied in detail, particularly their changes during storage in different conditions. Fruit species were described more closely in this study. The theoretical part is focused on ripening and storage of the fruits. The most common diseases of apples and pears are introduced too. In the practical part changes of some antioxidant levels in apples Idared (red-yellow) and Golden Delicious (green-yellow) were analyzed. Apples were stored for 2 months in three various storage conditions – at standard ambient temperature in the lab, in cellar and in refrigerator. In regular intervals samples were taken and levels of low molecular antioxidants (vitamin C, total phenolics, total flavonoids) and total antioxidant activity in apple fruits were analysed and compared. A surface microflora of the apples was analysed during the whole storage time. The most suitable storage conditions exhibited cellar (9 °C, 38,4 %). Phenolic changes in apples were substantially lower than flavonoid changes, while ascorbate content decreased dramatically with the storage time. Antioxidant activity was related mainly to ascorbate levels, influence of flavonoid changes was observed too. Antioxidant parameters were analyzed also in pear fruits, whose were stored only for very short time.
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Analysis of phenolics in different kinds of coffee
Jašková, Martina ; Halienová, Andrea (referee) ; Márová, Ivana (advisor)
The aim of this bachelor thesis was examining the quantity of biologically significant substances in selected types of roasted ground coffee, instant coffee and coffee extract. The particular kinds of coffee differed in technological processing, degree of roasting and composition. In the practical part total phenolics and flavonoids by using spectrophotometry methods. The total antioxidant activity in the individual coffee extracts was determined by ABTS method. Qualitative and quantitative analysis of some individual antioxidants and caffeine was performed using RP-HLPC with spectrophotometric detection. As a part of the work sensory analysis of all coffee samples was realized and consumer questionnaires were completed.
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Proteome and Metabolome Changes in Selected Organisms under Stress
Halienová, Andrea ; Kráčmar, Stanislav (referee) ; Čertík, Milan (referee) ; Šucman, Emanuel (referee) ; Márová, Ivana (advisor)
Living conditions of every organism are influenced by various factors at this time. Some of them have positive effect on organism, some negative. Basic condition for surviving is the ability to resist and adapt to changing metabolic and living conditions. Every single stress effect can lead to changes in metabolism but organisms have ability to develope sufficient mechanisms for stress response. Some of them are similar for all living organisms (enzyme production, endogenous primary stress metabolites) some of them are specific for certain organism or stress type. Cell stress response can be observed on different levels (proteomic, genomic, metabolomic). In proper conditions it can be used indrustrially. In this work, influences of various stress factors were studied. These factors were applied on selected organisms – carotenogenic yeast and plant materials. Yeast stress response was induced by osmotic and oxidation stress factors. Changes on proteomic level and in production of selected secondary metabolites were observed. Proteome was analyzed by 1D and 2D electrophoresis with subsequent analysis of proteins by mass spectrometry. Yeast strain Rhodotorula glutinis CCY 20-2-26 showed the best adaptation to stress factors, which was moreover accompanied by overproduction of carotenoids. This finding can be premise for next industrial production of carotenoids. In plant samples predominantly enzymes and metabolites involved in antioxidant response were studied.
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Analysis of phenolics in different kinds of coffee
Jašková, Martina ; Halienová, Andrea (referee) ; Márová, Ivana (advisor)
The aim of this bachelor thesis was examining the quantity of biologically significant substances in selected types of roasted ground coffee, instant coffee and coffee extract. The particular kinds of coffee differed in technological processing, degree of roasting and composition. In the practical part total phenolics and flavonoids by using spectrophotometry methods. The total antioxidant activity in the individual coffee extracts was determined by ABTS method. Qualitative and quantitative analysis of some individual antioxidants and caffeine was performed using RP-HLPC with spectrophotometric detection. As a part of the work sensory analysis of all coffee samples was realized and consumer questionnaires were completed.
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Changes of the content of some active substances in aplle and pear fruits during storage
Matějková, Markéta ; Halienová, Andrea (referee) ; Márová, Ivana (advisor)
In presented bachelor thesis low molecular substances antioxidants found in plant food were described. Antioxidants from apples and pears were studied in detail, particularly their changes during storage in different conditions. Fruit species were described more closely in this study. The theoretical part is focused on ripening and storage of the fruits. The most common diseases of apples and pears are introduced too. In the practical part changes of some antioxidant levels in apples Idared (red-yellow) and Golden Delicious (green-yellow) were analyzed. Apples were stored for 2 months in three various storage conditions – at standard ambient temperature in the lab, in cellar and in refrigerator. In regular intervals samples were taken and levels of low molecular antioxidants (vitamin C, total phenolics, total flavonoids) and total antioxidant activity in apple fruits were analysed and compared. A surface microflora of the apples was analysed during the whole storage time. The most suitable storage conditions exhibited cellar (9 °C, 38,4 %). Phenolic changes in apples were substantially lower than flavonoid changes, while ascorbate content decreased dramatically with the storage time. Antioxidant activity was related mainly to ascorbate levels, influence of flavonoid changes was observed too. Antioxidant parameters were analyzed also in pear fruits, whose were stored only for very short time.
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Biologically significant substances in cereal products
Vondráčková, Hedvika ; Halienová, Andrea (referee) ; Márová, Ivana (advisor)
Presented work was focused on study of biologically significant substances in cereal products. In experimental part selected active substances in cereal bars were analyzed. The highest content of total phenolics, total flavonoids, total antioxidant capacity, procyanidin B2 and chlorogenic acid was measured in plums and blueberry bars. The highest level of reduced saccharides was evaluated in raspberry and cereal bars. Presence of main simple sugars - maltose, glucose and fructose was detected in all bar samples.
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