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Interactions between plants and soil biota and effect of energetic crops on these interactions
Heděnec, Petr ; Frouz, Jan (advisor) ; Pergl, Jan (referee) ; Mortimer, Simon (referee)
My PhD thesis is focused on study of plant-soil interactions. Chapter 1 is focused on general introduction to the interactions between plants and soil biota in terms of relationships between soil organisms and aboveground biomass as well as root biomass. Chapter 2 is focused on food interaction of Folsomia candida and soil microscopic fungi (Penicillium chrysogenum, Penicillium expansum, Absidia glauca, and Cladosporium herbarum). Fungi were grown separately on Petri dishes or on different litter type (oak, alder and willow) separately as well. Laboratory experiments showed that food preference was more influenced by different litter types than fungal species. In chapter 3 was studied long-term production of hybrid sorrel and its effect on the composition of the soil meso and macrofauna, basal soil respiration, microbial biomass and composition of cultivable fraction of soil microscopic fungi in comparison with oilseed rape and cultural meadow. There was found that long-term production of hybrid sorrel affects the composition of soil fauna, while the microbial activity of the soil was more affected by agriculture practices, especially tillage. In chapter 4 was studied the effect of native (Salix viminialis and Phalaris arudinacea) and introduced (Reynoutria sachalinensis, Silphium perfoliatum and...
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Screening of possibilities of deep subsurface microflora to decompose selected organic compounds
Kuanysheva, Assel ; Frouz, Jan (advisor) ; Kryštůfek, Václav (referee)
Screening of possibilities of deep subsurface microflora to decompose selected organic compounds Abstract The aim of the study is to test the deep microflora bacterial strains for their ability to grow in oily environment, aliphatic hydrocarbons and toluene were taken as examples of aromatic hydrocarbons and where the cultivation of selected strain, were produced for testing its growth and microbial activity of selected strains in conditions simulating soil conditions; assess the usability these strains in practical remediation of contamination by oil. This thesis deals with the evaluation of possible use of selected strains of deep microflora for oil decomposition. It is evident, that some groups of microorganisms living in the Tertiary claystones at depths of 30-450 m below the surface are the biodegradable fossil organic matter type of kerogen. Chemical findings indicate that, this organic matter consists of various lengths of aliphatic chains, and thus the assumption that microorganisms decomposing kerogen might be able to disassemble oil and petroleum products. The findings of our experiment indicate that benzene and toluene, as well as kerogen are highly resistant to organic compounds and evidence of microbial degradation are rare. Utilization of oil as representative aliphatic compounds is better...
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Biotic interaction and their role in organic matter turnover in soil
Radochová, Petra ; Frouz, Jan (advisor) ; Smrž, Jaroslav (referee)
The thesis brings overview of main taxa of soil organisms. It describes in detail their trophic and non trophic interactions that contribute to organic matter decomposition in soil. In particular soil food web and behavior of fungal and bacterial channel of food web is described in relation to organic matter decomposition, soil formation and humus formation. This work contains also experimental work sealing with interaction of soil microscopic fungi and invertebrates. Food preference and suitability for development of three fungal species growing on three different litter types was studied. Litter was more important for food preference and food suitability. There were disproportion between litter suitability and litter preference. In particular oak was not preferred by adults but very suitable for development.
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Th effect of fertilization oan microbial and chemical soil properties in post mining sites.
Hermová, Markéta ; Frouz, Jan (advisor) ; Kryštůfek, Václav (referee)
The effects of fertiliser application, recultivation type and site age on chemical and microbiological soil properties were examined on a short-term (1year) experimental trial situated on spoil heaps in Sokolov mining district, Czech Republic. Treatments at the site included fertilisation by inorganic form of nitrogen, concretely calcic saltpetre (N) and fertilisation by inorganic form of phosphorus, namely superphosphate (P). Plots were either fertilised in the beginning of growing season or unfertilised. Microbial biomass-C increased as a consequence of both N and P addition while comparing with control. There was a significant effect of fertilisation on non-fumigated C representing labile source of carbon. With N,P application non-fumigated C significantly decreased. Specific respiration rate calculated as basal microbial respiration to microbial biomass-C was significantly increased by fertiliser application. In contrary, no significant treatment effect on the other parameters include water extractable carbon, total C, total N, available P, soil pH and basal respiration was recorded. The results indicate that short-term fertilisation is not able maintain soil condition. Therefore, site age or recultivation treatment had significant effect on mostly investigated parameters (WEC, total C) rather...
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Methods for distinguishing fossil and recent organic matter in post mining soils
Vindušková, Olga ; Frouz, Jan (advisor) ; Jehlička, Jan (referee)
Soil organic matter (SOM) has many important effects on soil and ecosystem quality. Soils developing on post-mining sites are characteristic of low recent SOM, but can contain reasonable amounts of fossil SOM originating from coal, kerogen or other fossil component of the overburden. With time, as vegetation develops, SOM accumulates and improves overall quality of post-mining soils. To assess soil quality development and quantify sequestered carbon, a reliable method for distinguishing recent and fossil SOM is needed. The relative amounts of recent and fossil carbon can be successfully quantified by the radiocarbon method, but due to it's high cost, it's common application is unlikely. The aim of this study was to (1) quantify the amount and variability of fossil SOM in post-mining soils near Sokolov, (2) compare different other SOM quantification methods to this reference, (3) test the potential of near infrared spectroscopy for recent and fossil carbon quantification. Fossil carbon accounts for 13 to 99 % of total organic carbon in studied soils, which is equivalent to 2 - 6% carbon content. Subtracting the carbon content of a deeper horizon (50 cm) from the content of A horizon provides a good estimate of recent carbon. This can be applied to carbon contents from dry combustion, wet dichromate...
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Assessing the impact of attendance on the selected caves using GIS
Kukla, Jaroslav ; Matějíček, Luboš (advisor) ; Frouz, Jan (referee)
Caves are valuable sites from geological , palaeontological and biological point of view. They are important biotopes of rare and endangered species. Their ecosystem is quite specific and often very independent on environment. Therefore, the caves since the beginning of science have been the subject of research , and gradually also the subject of protection. Caves can also be very attractive objects of interest of tourism. In the Czech Republic there are 14 open to the public karst caves that have regulated attendance, and their access time is subordinated to the interests of nature conservation. On the other hand, there are also a number of caves that are not secured in any way and may be a target of unregulated tourism. This dissertation discusses the evaluation and the impact of attendance on non-karst caves in the National Nature Reserve Canyon Labe, which are freely accessible. I divided the work into three parts. In the first part I dealt with quantifying the attendance of all caves in the area of interest, On the basis of collected data, I made a selection of caves , which I later focused on. In the second part of my work I focused on obtaining information about the visitors and their motivations using public inquiry survey in selected caves, In the third part I tried to analyze the impact...
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Nutrient budget in reclaimed and non reclaimed heaps
Veselá, Monika ; Frouz, Jan (advisor) ; Svoboda, Miroslav (referee)
Extraction of mineral resources causes landscape degradation and the aim of reclamation efforts is to restore ecosystems. Our research was carried out on spoil heaps after brown coal mining near Sokolov (Czech Republic). We compared twenty years old spontaneously developed sites and reclaimed sites afforested by alders Alnus glutinosa and A. incana. Biomass and nutrient budget were evaluated for herbs and woody plants, separately for species Salix caprea, Populus tremula and Alnus glutionosa. Alder showed significantly higher concentrations of nitrogen and carbon compared to other tree species. The total amount of biomass, carbon and nitrogen was higher on reclaimed sites whereas phosphorus was more represented on spontaneously developed sites. Most of nitrogen was accumulated in the below-ground tree biomass and the largest amount of phosphorus was in the above-ground tree biomass. Woody plants of non-reclaimed sites produced more litter whilst litter of alder stands showed a higher concentration of nitrogen. Higher values of biomass, carbon and nitrogen of reclaimed sites were probably achieved through alder ability to fix atmospheric nitrogen.
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Factors affecting oak spreading on post mining heaps
Janoušová, Ivana ; Frouz, Jan (advisor) ; Svoboda, Miroslav (referee)
The study was conducted on spoil heap Podkrušnohorská (50 ř 14'09 S, 12 ř 39'05 V) near Sokolov. Several factors that influence the establishment of oak were studied: the distance from the source of diaspors, type of habitat (succession vs alder reclamation), position of seedling on the wave and impact of animal grazing. Four alder restoration areas and three spontaneous succession areas have been mapped with GPS equipment, the occurence of mature oaks, which could serve as a source of acornsg was also mapped on the dump and in surroundings. Most seedlings (657) were found in the succession and only 98 in alder reclamation, and the occurence in succession was statistically significantly more frequent. The number of seedlings from sources diaspors decreased, the longest distance was over 1300 meters. For three habitats (spontaneous succession, alder reclamation, open space with grass vegetation (Calamagrostis epigeios)) were planted 12 oak seedlings in fence and 12 seedlings outsider of the fence. Annual growth rate and survival of oak were evaluated using two-way ANOVA. Results showed that the area has a significant effect on the growth of oaks. In alder reclamation and the open area had a high mortality of seedlings and small increase, the fence does not have significant influence on these areas....
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Changes of vegetation composition in hilly country forests depending on the application of historical forms of management
Nechanská, Denisa ; Boublík, Karel (advisor) ; Frouz, Jan (referee)
The aim of my study is to determine the influence of historical management practices on the camposition of vascular species in hilly country forests on the Vysoká stráň hill in the Bohemian Karst Protected Landscape Area in the Czech Republic. My thesis is divided into two parts. The first part of my thesis describes influences of forest management on the herb diversity from different scientific studies. There is some information about the influence of herbivorous grazing, pollarding, litter raking and fire. Secondly provides a case study dealing with the influence of historical management on the vascular species composition in the forest understory. My experimental area was divided into 32 treatments, where three different ways of historical management were used - mowing of the understory vegetation, litter raking and the combination of mowing and litter raking. The fourth area was a control treatment. Change in the herb diversity depending on a time is statistically significant but changes in the herb diversity depending on different ways of historical management are statistically insignificant. We detected a distinct decline in the herb diversity during our experiment, also on the control treatments. The main gradient of vegetation points from mesophilous acidophytes to species characteristic...
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Soil organic matter accumulation and humification during pedogenesis
Inogamova, Malika ; Frouz, Jan (advisor) ; Bartuška, Martin (referee)
Soil is comprised of minerals, SOM , water, and air. Soil development is caused by climate and living matter acting upon parent material (weathered mineral or organic matter from which the soil develops), as conditioned by topography, over time. SOM is a dynamic component of the soils system. Plant productivity is effected by the SOM. SOM provide nutrients and habitat to the organisms living in the soil, including plants, roots and SOM also binds soil particles into aggregates and improves the water holding capacity of soil. SOM is a product of biological decomposition that affects the chemical and physical properties of the soil and its overall fertility. Humus is only partly metabolized by soil organisms but improves the physical and chemical properties of soil. It consists of different humic substances: fulvic acid, humic acid, and humin. Soil is a solid material that results from the interaction of weathering and biological activity on the parent material or underlying hard rock. The fundamental pedogenic processes, used also as a criterion for classifying soils, is associated with SOM accumulation. We discuss the nature and interrelations of the SOM humification and accumulation processes during pedogenesis and the environmental factors on pedogenic processes influencing the rate of SOM...
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