|
|
Influence of glutamate-induced obesity on detoxification enzymes contains glutathione
Duchačová, Kateřina ; Boušová, Iva (advisor) ; Szotáková, Barbora (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Kateřina Duchačová Supervisor: doc. PharmDr. Iva Boušová, Ph.D. Title of rigorous thesis: Influence of glutamate-induced obesity on detoxification enzymes contains glutathione Obesity and related comorbidities occur more frequently in the population and belong to the most studied contemporary health topics. Aging of the organism is much discussed topic as well. Various animal models are used to observe changes in the organism at the organ, tissue, cellular and molecular levels. Comparison of specific activities and protein expressions of glutathione peroxidase (GPx) and glutathione reductase (GR), and specific activity of glutathione-S-transferase (GST) in the liver of obese mice and healthy controls according to the pathological state, aging and sex was the aim of this study. Mice with monosodium glutamate (MSG)-induced obesity, osmolal controls (NaCl) and mice without treatment of both sexes and at 8 and 20 weeks of age were used. Specific enzyme activities were determined spectrophotometrically. Protein expressions were determined using polyacrylamide gel electrophoresis followed by immunoblotting. The biggest obesity-related changes in enzyme activity were observed in GST, which activity...
|
|
|
Influence of glutamate-induced obezity on antioxidant enzymes in mice
Duchačová, Kateřina ; Boušová, Iva (advisor) ; Matoušková, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kateřina Duchačová Supervisor: doc. PharmDr. Iva Boušová, Ph.D. Title of diploma thesis: Influence of glutamate-induced obezity on antioxidant enzymes in mice. Obesity is currently one of the most widespread chronic diseases in the world. Many scientific teams are trying to clarify the causes of this disease and also examine changes in the body that may be related to this disease. The aim of this study was to determine the activity and expression of the enzymes superoxide dismutase (SOD) and catalase (CAT) and to assess activity of peroxidase (Px) in liver cytosol of experimental NMRI mice with obesity induced by monosodium glutamate (MSG) and healthy controls. Activities and expressions of selected enzymes were compared with respect to the pathological conditions (MSG mice, osmolality controls and unaffected mice), age (8 weeks x 20 weeks) and sex of the experimental mice. Activities were determined using spectrophotometric methods and polyacrylamide gel electrophoresis followed by immunoblotting was employed to monitor protein expressions. Differences in specific activities of SOD and CAT among the groups were only small. The most significant changes related to the induction of obesity...
|
|
|
Influence of glutamate-induced obesity on detoxification enzymes contains glutathione
Duchačová, Kateřina ; Boušová, Iva (advisor) ; Szotáková, Barbora (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Kateřina Duchačová Supervisor: doc. PharmDr. Iva Boušová, Ph.D. Title of rigorous thesis: Influence of glutamate-induced obesity on detoxification enzymes contains glutathione Obesity and related comorbidities occur more frequently in the population and belong to the most studied contemporary health topics. Aging of the organism is much discussed topic as well. Various animal models are used to observe changes in the organism at the organ, tissue, cellular and molecular levels. Comparison of specific activities and protein expressions of glutathione peroxidase (GPx) and glutathione reductase (GR), and specific activity of glutathione-S-transferase (GST) in the liver of obese mice and healthy controls according to the pathological state, aging and sex was the aim of this study. Mice with monosodium glutamate (MSG)-induced obesity, osmolal controls (NaCl) and mice without treatment of both sexes and at 8 and 20 weeks of age were used. Specific enzyme activities were determined spectrophotometrically. Protein expressions were determined using polyacrylamide gel electrophoresis followed by immunoblotting. The biggest obesity-related changes in enzyme activity were observed in GST, which activity...
|
|
|
Influence of glutamate-induced obezity on antioxidant enzymes in mice
Duchačová, Kateřina ; Boušová, Iva (advisor) ; Matoušková, Petra (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kateřina Duchačová Supervisor: doc. PharmDr. Iva Boušová, Ph.D. Title of diploma thesis: Influence of glutamate-induced obezity on antioxidant enzymes in mice. Obesity is currently one of the most widespread chronic diseases in the world. Many scientific teams are trying to clarify the causes of this disease and also examine changes in the body that may be related to this disease. The aim of this study was to determine the activity and expression of the enzymes superoxide dismutase (SOD) and catalase (CAT) and to assess activity of peroxidase (Px) in liver cytosol of experimental NMRI mice with obesity induced by monosodium glutamate (MSG) and healthy controls. Activities and expressions of selected enzymes were compared with respect to the pathological conditions (MSG mice, osmolality controls and unaffected mice), age (8 weeks x 20 weeks) and sex of the experimental mice. Activities were determined using spectrophotometric methods and polyacrylamide gel electrophoresis followed by immunoblotting was employed to monitor protein expressions. Differences in specific activities of SOD and CAT among the groups were only small. The most significant changes related to the induction of obesity...
|
guest ::
RSS feed.