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The study of VCAM-1 expression in experimental atherogenesis
Mertlíková, Veronika ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate: Veronika Mertlíková Supervisor: Doc. PharmDr. Petr Nachtigal Ph.D. Title of diploma thesis: The study of VCAM-1 expression in experimental atherogenesis. The aim of the thesis is to describe the expression of VCAM-1 in the vessel wall of normocholesterolemic C57BL/6J mice, which were fed standard laboratory diet, and hypercholesterolemic apoE-deficient mice. As an experimental model normocholesterolemic C57BL/6J male mice and apoE-deficient mice at the age of 6 weeks were used. The C57BL/6J male mice were fed standard laboratory diet and apoE-deficient mice were fed cholesterol diet for 8 weeks. After this procedure a biochemical analysis of blood and immunohistochemical analysis of segments taken from aortic sinus and aortal arch were made. In comparison with the control C57BL/6J mice a large level of cholesterol was found in the blood of the apoE-deficient mice. Atherosclerotic lesions are thus generated only by the hypercholesterolemic mice. We were monitoring the expression of VCAM-1 in aortas of the normocholesterolemic C57BL/6J mice without atherosclerotic lesions by immunohistochemical methods, and the same methods were used for monitoring of the areas with and without...
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Immunohistochemical Analysis of Expression of Phosphorylated form Smad2 after Administration of Atorvastatin in Experimental Atherogenesis
Vybulková, Lucie ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Candidate Mgr.Lucie Vybulková Consultant Doc.PharmDr.Petr Nachtigal, PhD Title of Thesis Immunohistochemical analysis of expression of phosphorylated form Smad2 after administration of atorvastatin in experimental atherogenesis Background: The aim of this thesis was to describe the effects of atorvastatin treatment on the expression of phosphorylated form of Smad2 in atherosclerotic plaques in ApoE/LDLr-deficient mice by immunohistochemical methods. Furthermore, the influence of atorvastatin on lipid spectrum and size of atherosclerotic plaques was observed. Methods: In our experiment there were used C57BL/6J female mice with double deficit of apolipoprotein E and LDL receptor. There was performed the biochemical analysis of blood samples, histological staining with oil red for detection of tissue lipids, quantitative analysis of size of lesions and immunohistochemical analysis of samples containing semilunar valves and aorta for the detection of expression of Smad2. It was done using the method Avidin-Biotin and detection with DAB. Results: Using biochemical analysis we confirmed increased levels of total cholesterol and VLDL after the administration of atorvastatin. Smaller size of...
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The studying of some markers of transforming growth factor pathway in mouse model of atherosclerosis.
Pagurko, Lukáš ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Abstracts Lukáš Pagurko The studying of some markers of transforming growth factor pathway in mouse model of atherosclerosis. Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Pharmacy Background: We observed the expression of endothelial nitric oxide synthase (eNOS) in mice atherosclerotic plaque by using imunohistochemical methods. The aim was to prove nonlipid-lowering effects of atorvastatin to affect the expression of eNOS. Methods: Female apoE and LDLr deficient mice, aged 16 weeks were used in the study. Biochemical analysis of blood samples and imunohistochemical analysis of the tissue segments were performed. For identification of eNOS expression was used EnVision method with DAB visualization. Results: We found out in biochemical analysis that 8-weeks doses of atorvastatin significantly increases levels of overall cholesterol. Presence of atherosclerotic lesions was observed at all incisions in control group even in atorvastatin group, whereas aterom necrotic fibres was founded at a number of them. The administration of atorvastatin were lead to significant decrease of atherosclerotic lesions compared to untreated group. There were also rapid increase of eNOS expression especially on the surface of atherosclerotic lesions. Conclusion: The positive influence of...
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Spirulina Platensis effects on atherogenesis in mouse model of atherosclerosis
Žáková, Marie ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Marie Žáková Spirulina platensis effects on atherogenesis in mouse model of atherosclerosis Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Pharmacy Background: The aim of this diploma thesis was to evaluate possible antiatherogenic and antiinflammatory effects of Spirulina platensis in apoE-deficient mice. Methods: We used C57BL/6J male mice with deficiency of apolipoprotein E (apoE-/-) at the age of 3 months. The animals were divided into 2 groups (control and examined mice). Both groups were fed with cholesterol diet for 8 weeks. In Spirulina group, Spirulina platensis was added at dose of 20 mg/kg/day. The biochemical analysis was performed in order to discover the lipid spectrum of blood. There was also performed the histological staining with oil red in order to visualise the size of atherosklerotic leisons and to detect the lipids. The aim of the immunohistochemical analysis was to approve the amount of VCAM-1 expression. Results: Biochemical analysis did not demonstrate any significant decrease of total cholesterol expect from decreasing of LDL cholesterol. The presence of atherosclerotic lesions with lipids cumulation was illustrated by histological staining with oil red in both mice groups, the results of each group did not differ. In comparison with...
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The study of transforming growth factor receptor during atherogenesis in mouse model of atherosclerosis.
Vybulková, Lucie ; Nachtigal, Petr (advisor) ; Doleželová, Eva (referee)
Lucie Vybulková The study of transforming growth factor receptor during atherogenesis in mouse model of atherosclerosis Diploma thesis Charles University in Prague, Fakulty of Pharmacy in Hradec Králové Pharmacy Background: The aim of this diploma thesis was the establishment of methodology for detection of TGF-βRI, subtype ALK-5, and studying its expression in the aortic root in apoE/LDLr- deficient mice. Methods: Using female mice C57BL/6J strain with a double deficit of apolipoprotein E and LDL-receptor was performed biochemical analysis of blood specimens, histologic staining with oil red for detection of lipids in tissues and immunohistochemical analysis of segments of tissue from the aorta and the top half of the heart. To detect expression of ALK-5 was used EnVision method with DAB visualization. Results: Using biochemical analysis, we confirmed increased levels of cholesterol in mice fed cholesterol diet as compared with mice on standard diet. At histologic staining with oil red we detected increased formation of atherosclerotic lesions in mice with cholesterol diet. Immunohistochemical analysis of expression of ALK-5 we demonstrated high expression of ALK-5 in the tunica media and in atherosclerotic plaques with no significant difference in the two groups of mice. Conclusion: The results...
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Evaluace www stránek vybraných municipalit
Doleželová, Eva
This paper establishes ground for the redesign of Brno-střed city district web page. For the purpose of this study web pages of several municipalities were evaluated: Brno-střed city district, Nové Město na Moravě, Praha 1 and Plzeň. Study shows how the selected municipalities fulfill the legal regulations of the Act no. 106/1999 on free access to information and the Act no. 81/2006 Coll. which states that municipalities are obligated to provide the information on web pages also to handicapped or disabled. Furthermore the usability analysis and quantitative survey were applied to municipality web page in Brno-střed region to determine the public needs and requirements. Presented findings indicate both significant failure in fulfilling the law and public dissatisfaction with Brno-střed web page content and structure. This dissertation provides sample solutions for web page usability.
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Evaluation of the Financial Situation of a Company and Proposals For Its Improvement
Doleželová, Eva ; Kristián, Petr (referee) ; Sághy Estélyi, Kristína (advisor)
The goal of the submitted thesis "Evaluation of the financial situation and proposals for its improvement" is based on economic analysis, assessment of the performance of the selected enterprises Montáže inženýrských sítí spol. s r.o. and identify the weaknesses of the company and then propose solutions to eliminate them. The first part is defined to theoretical and methodological bases of economic analysis. In the second part are then selected methods applied to a specific company. In this work are used to evaluate the economic performance of enterprises methods of financial analysis, PEST analysis, Porter’s five forces analysis and SWOT analysis. The results of the calculations is the synthetic part of the work assessed and management measures designed to improve the financial situation of the company.
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Preparing high school students for the university studies in the area of natural science
DOLEŽELOVÁ, Eva
This bachelor thesis focuses on issues and aspects of science education in secondary schools. The theoretical section describes available options for selecting appropriate teaching methods, including additional student activities such as Olympiads and student research projects. The language skills of high school students are also analyzed. The practical section describes a conducted exploratory probe focused on finding information about the knowledge of high school students on student research and professional activities, Olympiads, and their opinion on the importance of these activities for the University entrance examination. Finally, the listed requirements of selected universities to study applicants are discussed.
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