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HMGB1 in bacterial infections.
Chmelařová, Petra ; Jílek, Petr (advisor) ; Celer, Vladimír (referee)
Bc. Petra Chmelařová Title of diploma thesis: HMGB1 in bacterial infections Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Background: Detection of HMGB1 mRNA expression and HMGB1 at the protein level in blood and ileum during bacterial infection in gnotobiotic piglet as infection model. Evaluation of the course of infection with expression IL-8, IL10, TNF-α and TLR4 mRNA in ileum and IL- 8, IL10 a TNF-α levels in blood and intestinal washes. Final assessment of HMGB1 as potential late marker of sepsis in pig. Methods: 1) The groups of gnotobiotic piglets - germfree (control), infected (extracellular parasite E.coli O55 or intracellular parasite Salmonella enterica serogroup Typhimurium) and associated piglets (probiotic E.coli Nissle 1917), 2) determination of bacterial count as CFU in blood and intestine with cultivation on bacteriological media, 3) column-based total RNA isolation from intestine, 4) spectrophotometric RNA quantification and estimation of purity, 5) cDNA synthesis with combined primers (oligo d(T) and random n-mers), 6) real-time polymerase chain reaction with LNA probes, 7) real-time PCR data normalization to β-actin and relativization to mean value of gene transcription, 8) ELISA detection of HMGB1 and selected...
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Economic Conditions for Sport Centre Foundation
Vojáčková, Jana ; Janák, Vladimír (advisor) ; Chmelařová, Petra (referee)
Title: Economic Conditions for Sport Centre Foundation Objectives: Objectives of this thesis is to set up establishment model of sport facility in border town of České Velenice. To achieve the goal it is necessary to define individual steps and to describe the activities related to foundation of new business entity. The intention is to create a sport offer that is missing in the town. This offer would fill the gap in the market and it would also reflect requirements of current and potential customers. The point is to determinate an entrepreneurial vision. Methods: In this thesis methods of descriptive analysis, observation and inquiry were used. Methods - descriptive analysis and observation - were applied to characteristics of the town of České Velenice with the objective to evaluate preconditions for the new business entity. Crucial tool to find out wishes and needs of residents within considered sport facility was implementation of written questionnaire. SWOT analysis was afterwards used for final assessment of risks and anticipated successes. Results: Based on the characteristics of the town of České Velenice and written inquiry was found that in the town and its surroundings there is no device that offers access to excellent sports facilities. There are enough people interested in sport as...
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Egypt as a destination of tourism with a focus on sport activities offers
Chmelařová, Petra ; Navrátilová, Miroslava (advisor) ; Šíma, Jan (referee)
Title: Egypt as a destination of tourism with a focus on sport activities offers. Project goals: To create the offer of the tours with view to the sport activities in Egypt destination. Method: Descriptive analysis, SWOT analysis, comparative analysis, method of observation Results: The results give the comprehensive survey of the Egypt destination and offer of the sports tours, design new possibilities of active holiday. Key words: Tourism, Egypt, sports, tours
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The development of legal, political and social aspects of obscenity in the USA
Chmelařová, Petra ; Calda, Miloš (advisor) ; Korytová Magstadt, Štěpánka (referee)
Diploma thesis "The development of legal, political and social aspects of obscenity in the USA" focuses on the historical development of the basic definition of obscenity, highlights the long and difficult process of its formulation and presents crucial decisions of the Supreme court. It also briefly summarizes chief arguments supporting or rejecting censorship on the grounds of obscenity. This work also provides with a general overview of legislature concerning obscenity in the United States and concisely presents some interest groups, which are actively fighting against obscenity/ indecency or for the protection of the freedom of speech. Furthermore, the work also focuses on the work of the Federal Communication commission and gives an overview of its rulings regulating obscenity and indecency in broadcasting media. Finally, this work also introduces the Nipplegate scandal and concentrates on the developments after it.
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Economic Conditions for Sport Centre Foundation
Vojáčková, Jana ; Janák, Vladimír (advisor) ; Chmelařová, Petra (referee)
Title: Economic Conditions for Sport Centre Foundation Objectives: Objectives of this thesis is to set up establishment model of sport facility in border town of České Velenice. To achieve the goal it is necessary to define individual steps and to describe the activities related to foundation of new business entity. The intention is to create a sport offer that is missing in the town. This offer would fill the gap in the market and it would also reflect requirements of current and potential customers. The point is to determinate an entrepreneurial vision. Methods: In this thesis methods of descriptive analysis, observation and inquiry were used. Methods - descriptive analysis and observation - were applied to characteristics of the town of České Velenice with the objective to evaluate preconditions for the new business entity. Crucial tool to find out wishes and needs of residents within considered sport facility was implementation of written questionnaire. SWOT analysis was afterwards used for final assessment of risks and anticipated successes. Results: Based on the characteristics of the town of České Velenice and written inquiry was found that in the town and its surroundings there is no device that offers access to excellent sports facilities. There are enough people interested in sport as...
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HMGB1 in bacterial infections.
Chmelařová, Petra ; Celer, Vladimír (referee) ; Jílek, Petr (advisor)
Bc. Petra Chmelařová Title of diploma thesis: HMGB1 in bacterial infections Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Background: Detection of HMGB1 mRNA expression and HMGB1 at the protein level in blood and ileum during bacterial infection in gnotobiotic piglet as infection model. Evaluation of the course of infection with expression IL-8, IL10, TNF-α and TLR4 mRNA in ileum and IL- 8, IL10 a TNF-α levels in blood and intestinal washes. Final assessment of HMGB1 as potential late marker of sepsis in pig. Methods: 1) The groups of gnotobiotic piglets - germfree (control), infected (extracellular parasite E.coli O55 or intracellular parasite Salmonella enterica serogroup Typhimurium) and associated piglets (probiotic E.coli Nissle 1917), 2) determination of bacterial count as CFU in blood and intestine with cultivation on bacteriological media, 3) column-based total RNA isolation from intestine, 4) spectrophotometric RNA quantification and estimation of purity, 5) cDNA synthesis with combined primers (oligo d(T) and random n-mers), 6) real-time polymerase chain reaction with LNA probes, 7) real-time PCR data normalization to β-actin and relativization to mean value of gene transcription, 8) ELISA detection of HMGB1 and selected...
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Egypt as a destination of tourism with a focus on sport activities offers
Chmelařová, Petra ; Šíma, Jan (referee) ; Navrátilová, Miroslava (advisor)
Title: Egypt as a destination of tourism with a focus on sport activities offers. Project goals: To create the offer of the tours with view to the sport activities in Egypt destination. Method: Descriptive analysis, SWOT analysis, comparative analysis, method of observation Results: The results give the comprehensive survey of the Egypt destination and offer of the sports tours, design new possibilities of active holiday. Key words: Tourism, Egypt, sports, tours
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