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National Repository of Grey Literature

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	Caspase 3 chemiluminiscence activity determination in apoptotic cells and design of Caspase 3 sensor Lišková, Marcela ; Klepárník, Karel ; Pazdera, P. ; Foret, František Apoptosis, or programmed cell death, is an essential physiological process that plays a critical role in development of tumor or autoimmune disorders. In some instances knowledges of this pathway can be used for inhibit or prevent cancer development and other diseases. One of the earliest and most consistent observed features of apoptosis is the induction of a series of cytosolic proteases – caspases. Detailed record
	Sensor based on Förster resonance energy transfer with quantum dot Lišková, Marcela ; Datinská, Vladimíra ; Klepárník, Karel ; Foret, František Förster resonance energy transfers (FRET) are based on the nonradiative transfer of energy between a donor and acceptor dye in the distance 1-10 nm. With respect to this, quantum dots (QDs) with a high absorbance can serve as suitable donors of energy in FRET. Here, we present architectures of two sensors based on QD conjugates useful in imaging and diagnostics of cancer. One sensor, based on an oligonucleotide conjugate, can be used for the detection of complementary oligonucletide chains. The other was designed for the detection of enzymes inside cells. Detailed record
	CECE 2011. 8th International Interdisciplinary Meeting on Bioanalysis Foret, František Sborník obsahuje program, abstrakta 17 zvaných přednášek s krátkou informací o profesionální dráze řečníků a abstrakta posterů. Detailed record
	Preparation of highly luminescent CdTe quantum dots and its probes Voráčová, Ivona ; Lišková, Marcela ; Patrmanová, M. ; Klepárník, Karel ; Foret, František Luminescence probes consisting of quantum dots conjugated with macrocyclic ligand complex were characterized by CE-LIF. Detailed record
	Luminescence of nanoparticles - quantum dots Voráčová, Ivona Compilation of materials, physical-chemical properties, synthesis and applications of quantum dots. Detailed record
	Immunofluorescent labeling of tissues and free cells via QD-protein conjugates Lišková, Marcela ; Voráčová, Ivona ; Hezinová, Věra ; Klepárník, Karel ; Foret, František Preparation of quantum dots and using their QD-protein conjugates in immunofluorescent labeling of cells and tissues. For conjugation was used method viaEDC/sulfo-NHS zero-length cross-linkers. We linked QD with annexin V, anti-CD3 and anti-PCNA. We labeled T-lymphocytes, apoptotic lymphocyte, mouse duodenum tissue and tumor tissue. Detailed record
	Preparation, physical-chemical properties and utilization of nanoparticles in bioanalysis Svobodová, Ivona ; Hezinová, Věra ; Lišková, Marcela ; Přikryl, Jan ; Maděránková, D. ; Klepárník, Karel ; Foret, František The possibilities of application of nanoparticles in bioanalysis were studied. Applications of quantum dots as a fluorescent markers, silver nanoparticles in SERS and conjucation of gold nanoparticles with antibodies were shown. Detailed record
	Application of nanoparticles in fluorescence microscopy and raman spectrometry Přikryl, Jan ; Svobodová, Ivona ; Hezinová, Věra ; Lišková, Marcela ; Maděránková, D. ; Klepárník, Karel ; Foret, František This contribution is devoted to application of quantum dots as perspective luminescence labels in fluorescence microscopy (epifocal and TIR fluorescence microscopy) and application of silver nanoparticles in surface-enhanced Raman spectroscopy. Detailed record
	CdTe kvantové tečky - příprava, fyzikálně-chemické vlastnosti a aplikace jako fluorescenční značky Hezinová, Věra ; Svobodová, Ivona ; Lišková, Marcela ; Přikryl, Jan ; Klepárník, Karel ; Foret, František The set of different sizes of CdTe nanoparticles coated with MPA was synthetized and characterized by fluorescence spectra and lifetimes. Their application as a cell fluorescent markers was tested. Detailed record
	Surface enhanced Raman spectroscopy Maděránková, D. ; Provazník, I. ; Klepárník, Karel The signal of Raman scattering in carotene solutions, enhanced on silver colloid particles was studied. The dependence of the enhancement factor on the concentration of silver particles demonstrates the potential of the method for biological applications. Detailed record

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