National Repository of Grey Literature 21 records found  1 - 10nextend  jump to record: Search took 0.01 seconds. 
The evaluation of the microbial diversity in anthropogenic subtrates using a method analyzing the polymorphism of the terminal restriction fragments of DNA
Gryndler, Milan ; Bukovská, Petra ; Sýkorová, Zuzana ; Havránková, Marie
In this methodology we describe a fast, easy and non-expensive method for evaluation of microbial diversity in various types of substrates: T-RFLP. We explain principles and describe in detail the workflow of DNA extraction, amplification of a particular piece of DNA in PCR, choice of restriction enzymes and the restriction digest, separation of fragments using the capillary sequencer as well as discuss the evaluation and interpretation of the results.
Appropriate technology for recycling of phosphorus from surface water and wastewater
Holba, Marek ; Maršálek, Blahoslav ; Maršálková, Eliška ; Plotěný, Miroslav
There were identified the sources of phosphorus, which should be used to maximize the recycling procedures. To achieve these improvements new recycling technologies were introduced. Currently, there are quite a wide range of technology solutions, where every solution has its pros and cons. And no technology shows itself as clearly the best solution. However in the modern world are technologies focused on recycling procedures of phosphorus in the form of struvite and processing of sewage sludge. While for developing countries appears to be the most appropriate technology based on the separation of urine and manure from livestock.
Example use of electrocoagulation in outflow from wastewater treatment plant
Plotěný, Miroslav ; Maršálková, Eliška ; Maršálek, Blahoslav ; Holba, Marek
Based on the results of this study was showed that the electrocoagulation process has some advantages in comparison with other methods of phosphorus removal. Such as simple equipment, simple handling, short time delay, reduced chemical dosing or relatively rapid sedimentation flakes. The study demonstrated that the real potential of this technology is applicable mainly in small villages, where are not installed or operating other technologies for phosphorus removal.
Manipulation with flow rates in water bodies - the possibilities for inhibition of cyanobacterial blooms development
Maršálek, Blahoslav ; Šejnohová, Lenka ; Vinklárková, Darina ; Plotěný, Miroslav ; Jančula, Daniel
If we compare the sediment capping by active barriers to protect the phosphorus release from sediments with other technologies of manipulation with methods of water discharge, it is clear, that by well-considered manipulations with water discharge, it is possible to reach the same (comparable) results with lower costs and with lower ecotoxicological and limnological risks. By the manipulation of water level, it is possible to reduce cyanobacterial bloom development as supplemental or supporting measure, but the effects may differ in different water bodies and thus it must be consulted always with specialists.
The influence of urban water for the proliferation of cyanobacterial water bloom
Maršálek, Blahoslav ; Maršálková, Eliška ; Holba, Marek
Emergence spillways are very important for phosphorus catchment balance. Our measurememnts showed that one storm rainfall brings to the recipient so much phosphorus like two months of general inlet. Therefore, it is necessary to change sampling methods and plans.
On-line quantification of cyanobacteria in a raw water
Novotná, Zlatica ; Maršálek, Blahoslav ; Trtílek, M. ; Rataj, T.
A new fluorometer AOM (Algae Online Monitor) for the quantification of phytoplankton in raw water was tested on different localities. The AOM was serviceable in the variable field conditons. Because of low limit of detection and due the work in continuous mode with remote GSM data transfer to laboratory, the device is suitable for early warning system.
The effect of phosphorus deficiency on growth and primary processes of photosynthesis in algae and cyanobacteria
Zezulka, Štěpán ; Maršálek, Blahoslav
The management of cyanobacterial blooms is connected with the necessity to limit the input of nutrients into surface water reservoirs, especially phosphorus. Higher content of phosphorus or change in N/P ratio towards phosphorus give advance for cyanobacteria. Laboratory cultures of cyanobacteria Synechococcus and green alga Chlorella were cultivated in standard medium (0.18 mM P) and then transferred into medium without phosphorus or with 0.045 mM, 0.09 mM, 0.18 mM and 0.36 mM P. Growth rate (optical density) and parameters of induced chlorophyll fluorescence were measured repeatedly during 7 days of cultivation. Significant inhibitive effect on growth and fluorescence parameters was found in cyanobacteria cultivated in medium without P. On the other hand, green alga did not exhibit any significant sensitivity to the P concentration in the medium.
Detection and localization of cyanobacterial inoculum in the sediments - use knowledge of life strategies to minimize health risks
Šejnohová, Lenka ; Maršálek, Blahoslav ; Novotná, Zlatica ; Maršálková, Eliška
Water bloom (WB) is a global problem that is particularly closely linked with increased inputs of phosphorus to surface waters.. The subsequent accumulation of nutrients in the tank for the development not only of cyanobacteria in the water column, but also their survival in resting stages in the sediment. The quantity (amount) of inoculum (source) of cyanobacteria in the sediments is the primary factor, along with nutrient and climatic conditions, correlates with the resulting amount of algae in the water column during the summer season. Cyanobacterial resting stages in sediments require specific conditions for survival, which moreover genus from the genus (or species), cyanobacteria differ. Knowledge of the life cycle of cyanobacteria is therefore an essential prerequisite for starting a guide to environmentally friendly, with the current low cost of funds, limit the development of WB.
Elimination toxins of cyanobacteria
Jančula, Daniel ; Blahová, L. ; Maršálek, Blahoslav
Phthalocyanine molecules which are able to degrade microcystins - toxins, have been discovered.
Using of flow cytometry for the quantification and physiological state detection of aquatic microorganisms
Mikula, Přemysl ; Maršálek, Blahoslav
A paper informs about trends in flow-cytometric analysis of microbial populations and communities in aquatic environment. It describes methods of flow-cytometric analysis, advantages and disadvantages of flow-cytometric measurements as well as basic principles of flow cytometry.

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