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National Repository of Grey Literature 6 records found  Search took 0.01 seconds. 
Analysis of low abundant lipids in vernix caseosa using chromatographic methods and mass spectrometry
 Háková, Eva ; Míková, R. ; Vrkoslav, Vladimír ; Plavka, R. ; Cvačka, Josef
The aim of this work is to develop a separation method for characterization of unknown lipids in vernix caseosa. We focused on very low abundant lipids which have not been previously described in vernix caseosa. The total lipid extract was fractionated using low pressure normal phase liquid chromatography to obtain sub-fractions. The sub-fractions were further separated in high performance liquid chromatography mode to isolate classes of lipids. This procedure enabled us to characterise lipid classes by mass spectrometry and identify one of the class as acyl-ceramides with non-hydroxylated fatty acids.
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Separation of nonpolar lipids from vernix caseosa
 Háková, Eva ; Míková, Radka ; Vrkoslav, Vladimír ; Doležal, A. ; Plavka, R. ; Cvačka, Josef
The aim of this work is to develop a separation method for characterization of unknown nonpolar lipids in vernix caseosa. Here we optimized adsorption chromatography coupled to mass spectrometry detection with exact mass measurement. A long silica gel column (250 + 250 x 4.6 mm, particle size: 5 mu m) provided the best separation of nonpolar lipids present in vernix caseosa.
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Development and applications of ionization techniques in ambient mass spectrometry
 Rejšek, Jan ; Vrkoslav, Vladimír ; Cvačka, Josef
Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization techniques in the ambient mass spectometry. Herein, they were investigated in two research areas: (1) TLC analysis. (2) Localization of insect defense glands.
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Vernix caseosa and its newly discovered nonpolar lipids
 Háková, Eva ; Míková, Radka ; Vrkoslav, Vladimír ; Doležal, A. ; Plavka, R. ; Cvačka, Josef
The aim of this work is a description of newly, so far undescribed nonpolar lipids, which are components of vernix caseosa.
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Development and applications of ionization techniques in ambient mass spectrometry
 Rejšek, Jan ; Vrkoslav, Vladimír ; Cvačka, Josef
Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization techniques in the ambient mass spectometry. Herein, they were investigated in two research areas: (1) TLC analysis. (2) Localization of insect defense glands.
Detailed record
Mass spectrometry in lipidomics
 Cvačka, Josef
Current approaches of analytical chemistry in lipidomics based on mass spectrometry and chromatography are discussed in detail. Examples of qualitative, quantitative and imaging approaches are given.
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