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Function of the AtKUP5 transporter in Arabidopsis thaliana
Štočková, Hana ; Tylová, Edita (advisor) ; Hála, Michal (referee)
Potassium is one of the essential elements necessary for plant growth. It is involved in many plant processes, such as osmoregulation, enzymes activaton, etc. These functions are very often closely related to its transport in the cell and the whole plant. Although potassium is abundant in earth's crust, the mount of plant-available form is often insufficient. Potassium deficiency manifests itself on many levels and also contributes to the reduction of yield and quality of agricultural crops. There are many of potassium-transporting proteins in the plant. One of the important families of potassium transporters is the KT/HAK/KUP family. This family includes, among others, the high-affinity transporter HAK5, which is key for the uptake of potassium from the environment with low-potassium availability. One of the not very characterized transporters from the KT/HAK/KUP family is the KUP5 transporter, which I deal with in my diploma thesis. The aim of this work is to analyze the phenotypic manifestations of kup5 T-DNA insertion mutants and to characterize the functions of the transporter KUP5 in Arabidopsis thaliana plants. I analyzed the growth of kup5 insertion mutants in various environmental conditions and performed plant transformation to determine the localization of the KUP5 transporter in the...
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Function of the AtKUP5 transporter in Arabidopsis thaliana
Štočková, Hana ; Tylová, Edita (advisor) ; Hála, Michal (referee)
Potassium is one of the essential elements necessary for plant growth. It is involved in many plant processes, such as osmoregulation, enzymes activaton, etc. These functions are very often closely related to its transport in the cell and the whole plant. Although potassium is abundant in earth's crust, the mount of plant-available form is often insufficient. Potassium deficiency manifests itself on many levels and also contributes to the reduction of yield and quality of agricultural crops. There are many of potassium-transporting proteins in the plant. One of the important families of potassium transporters is the KT/HAK/KUP family. This family includes, among others, the high-affinity transporter HAK5, which is key for the uptake of potassium from the environment with low-potassium availability. One of the not very characterized transporters from the KT/HAK/KUP family is the KUP5 transporter, which I deal with in my diploma thesis. The aim of this work is to analyze the phenotypic manifestations of kup5 T-DNA insertion mutants and to characterize the functions of the transporter KUP5 in Arabidopsis thaliana plants. I analyzed the growth of kup5 insertion mutants in various environmental conditions and performed plant transformation to determine the localization of the KUP5 transporter in the...
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Potassium deficiency in plants - signs and responses
Štočková, Hana ; Tylová, Edita (advisor) ; Konrádová, Hana (referee)
Plants receive K+ mainly from the soil through the root system. In soil, K+ occurs primarily in minerals such as mica and potassium feldspar. The availability of K+ for plant uptake depends on the form in which K+ occurs in soil. There are forms directly available, slowly available, and unavailable for plant and the transition of K+ between these pools may occur. In plant, K+ is very mobile and it occurs in high amount in cells. It is the most prominent cytoplasmic cation. It affects high number of metabolic processes, including photosynthesis, osmoregulation, and activation of enzymes. K+ shortage can cause changes in plant morphology, anatomy and metabolism. K+ deficiency can be manifested by leaf deformation, decreased leaf area, necrosis, short internodes, reduced rate of photosynthesis, etc. K+ deficient plant is also more vulnerable to pathogens and its resistance to abiotic stress factors such as drought, low temperatures, or salinity is decreased. Deficiency of K+ reduces the biomass and overall yield in agricultural crops, so K+ fertilizers, both soil and foliar, are needed. The thesis focuses on the manifestations of K+ deficiency in plants and summarizes the recent findings on mechanisms of K+ deficiency perception and signal pathways leading to the response to this deficiency. Key...
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