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Evolution of the genetic code and classification of oxymonads
Šrámová, Eliška ; Hampl, Vladimír (advisor) ; Čepička, Ivan (referee)
Oxymonads are a group of heterotrophic flagellates living in low oxygen environment. These protists inhabit mainly the gut of xylophagous insects (cockroaches, termites), with an exception of the genus Monocercomonoides, which was described from the intestinal contents of many vertebrates. On the basis of molecular data, Oxymonadida are classified into the supergroup Excavata (Cavalier-Smith, 2002; Simpson et al., 2006, Hampl et al. 2009). This thesis was focused on the diversity of genus Monocecomonoides from the morphologically simplest family Polymastigidae. The main goal of our work was to gather sequence data from strains isolated from a wide spectrum of hosts. We have obtained 26 partial sequences of the gene for the SSU rDNA in total, of which two belonged to another oxymonad, apparently genus Oxymonas. Our phylogenetic analysis indicated that the representatives of the genus Monocercomonoides form one group, however with a low bootstrap support. On the basis of published data about the presence of non-canonical genetic code in some oxymonads (Keeling and Leander, 2003; de Koning et al., 2008), we decided to explore this rare phenomenon in representatives of the genus Monocercomonoides. For this part of the study we gathered 9 partial sequences of α-tubulin gene. In these sequences we have not...
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Study of the effect of cholesterol concentration on monolayer models
Šrámová, Eliška ; Paraskevopoulos, Georgios (advisor) ; Svačinová, Petra (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Author: Eliška Šrámová Supervisor: Dr. Georgios Paraskevopoulos, Ph. D Consultant: Mgr. Anna Nováčková Title of Thesis: Study of the effect of cholesterol concentration on monolayer models Skin is composed of 3 major layers: hypodermis, dermis, and epidermis. The uppermost layer, which is called stratum corneum (SC), has a unique structure resembling a well-built wall. Corneocytes represent bricks and the lipid matrix works like a mortar. Ceramides, free fatty acids, and cholesterol (Chol) are the main lipids creating the human SC matrix. In a healthy SC, the ratio of these lipids is equimolar. This ratio is crucial, not only to maintain the barrier function of the skin, but also for the organization of lipids in SC. Chol appears to be required for the correct lamellar organization, and the ordering of lipids inside of the lamellar formation (lateral organization) in SC. Abnormalities in the ratio of the lipids and depleted amount of Chol can lead to a disruption of the skin barrier function resulting in skin disease or multisystemic diseases (e.g. X-linked ichthyosis, Conradi-Hünermann-Happle syndrome, and CHILD syndrome.) The present work deals with a Chol deficiency study on lipid organization on...
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Study of the effect of cholesterol concentration on monolayer models
Šrámová, Eliška ; Paraskevopoulos, Georgios (advisor) ; Svačinová, Petra (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Author: Eliška Šrámová Supervisor: Dr. Georgios Paraskevopoulos, Ph. D Consultant: Mgr. Anna Nováčková Title of Thesis: Study of the effect of cholesterol concentration on monolayer models Skin is composed of 3 major layers: hypodermis, dermis, and epidermis. The uppermost layer, which is called stratum corneum (SC), has a unique structure resembling a well-built wall. Corneocytes represent bricks and the lipid matrix works like a mortar. Ceramides, free fatty acids, and cholesterol (Chol) are the main lipids creating the human SC matrix. In a healthy SC, the ratio of these lipids is equimolar. This ratio is crucial, not only to maintain the barrier function of the skin, but also for the organization of lipids in SC. Chol appears to be required for the correct lamellar organization, and the ordering of lipids inside of the lamellar formation (lateral organization) in SC. Abnormalities in the ratio of the lipids and depleted amount of Chol can lead to a disruption of the skin barrier function resulting in skin disease or multisystemic diseases (e.g. X-linked ichthyosis, Conradi-Hünermann-Happle syndrome, and CHILD syndrome.) The present work deals with a Chol deficiency study on lipid organization on...
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Study of the effect of cholesterol concentration on monolayer models
Šrámová, Eliška ; Paraskevopoulos, Georgios (advisor) ; Svačinová, Petra (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Author: Eliška Šrámová Supervisor: Dr. Georgios Paraskevopoulos, Ph. D Consultant: Mgr. Anna Nováčková Title of Thesis: Study of the effect of cholesterol concentration on monolayer models Skin is composed of 3 major layers: hypodermis, dermis, and epidermis. The uppermost layer, which is called stratum corneum (SC), has a unique structure resembling a well-built wall. Corneocytes represent bricks and the lipid matrix works like a mortar. Ceramides, free fatty acids, and cholesterol (Chol) are the main lipids creating the human SC matrix. In a healthy SC, the ratio of these lipids is equimolar. This ratio is crucial, not only to maintain the barrier function of the skin, but also for the organization of lipids in SC. Chol appears to be required for the correct lamellar organization, and the ordering of lipids inside of the lamellar formation (lateral organization) in SC. Abnormalities in the ratio of the lipids and depleted amount of Chol can lead to a disruption of the skin barrier function resulting in skin disease or multisystemic diseases (e.g. X-linked ichthyosis, Conradi-Hünermann-Happle syndrome, and CHILD syndrome.) The present work deals with a Chol deficiency study on lipid organization on...
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Evolution of the genetic code and classification of oxymonads
Šrámová, Eliška ; Hampl, Vladimír (advisor) ; Čepička, Ivan (referee)
Oxymonads are a group of heterotrophic flagellates living in low oxygen environment. These protists inhabit mainly the gut of xylophagous insects (cockroaches, termites), with an exception of the genus Monocercomonoides, which was described from the intestinal contents of many vertebrates. On the basis of molecular data, Oxymonadida are classified into the supergroup Excavata (Cavalier-Smith, 2002; Simpson et al., 2006, Hampl et al. 2009). This thesis was focused on the diversity of genus Monocecomonoides from the morphologically simplest family Polymastigidae. The main goal of our work was to gather sequence data from strains isolated from a wide spectrum of hosts. We have obtained 26 partial sequences of the gene for the SSU rDNA in total, of which two belonged to another oxymonad, apparently genus Oxymonas. Our phylogenetic analysis indicated that the representatives of the genus Monocercomonoides form one group, however with a low bootstrap support. On the basis of published data about the presence of non-canonical genetic code in some oxymonads (Keeling and Leander, 2003; de Koning et al., 2008), we decided to explore this rare phenomenon in representatives of the genus Monocercomonoides. For this part of the study we gathered 9 partial sequences of α-tubulin gene. In these sequences we have not...
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Genotyping of \kur{Giardia intestinalis} isolates
ŠRÁMOVÁ, Eliška
The aim of this work was assemble isolates of Giardia intestinalis from humans and other mammals. Stools samples were examined for presence of cysts by concentration settling method. Consequently sequencing of 532 bp parts of the TPI gene after previous amplification by the nested PCR was performed. In vitro cultures of selected isolates were established using experimental model hosts, gerbils.
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