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National Repository of Grey Literature 3 records found  Search took 0.01 seconds. 
The utility of Toll-like receptor 2 in defining the progenitors of definitive embryonic hematopoiesis
 Šplíchalová, Iva ; Filipp, Dominik (advisor) ; Nečas, Emanuel (referee) ; Kalina, Tomáš (referee)
Hematopoiesis is a vital process in which red blood cells and cells of the immune system are formed. It is initiated during early embryonic development when we find hematopoietic progenitors in separate anatomical sites. Embryonic hematopoiesis comprises three successive and partly overlapping waves of progenitors with a different hematopoietic potential. The primary anatomical place where hematopoiesis takes place shortly before the birth is the bone marrow (BM). Since at this time point of development BM is already populated by hematopoietic stem cell (HSCs) progenitors, it becomes also the site of hematopoiesis in adulthood. However, the bone marrow is not the only place where hematopoietic progenitors emerge and develop. The Yolk sac (YS) and the Aorta-Gonad-Mesonephros (AGM) region are the initial sites of the appearance of the three waves of progenitors in the early embryogenesis. These progenitors and their descendants play an indispensable role during the development of an individual. Because there are no specific markers that would unambiguously characterize progenitors of these individual waves, their physical separation and hence also functional characterization is still incomplete. Recent studies have shown that Toll-like receptors (TLRs) are expressed on adult HSCs. The stimulation of...
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Optimization of transfection of eukaryotic cell lines
 Šplíchalová, Iva ; Zemanová, Lucie (advisor) ; Štambergová, Hana (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Iva Šplíchalová Supervisor: RNDr. Lucie Škarydová, Ph.D. External supervisor: Mgr. Vojtěch Vejvoda, Ph.D. Title of Master thesis: Optimization of Transfection of Eukaryotic Cells An optimization of a transfection is a process of searching for the best conditions for the transfection. Expression systems corresponding to concrete requirements are chosen first of all. A speed, an economy and a functionality of prepared recombinant protein that can be influenced by posttranslational modifications must be considered. Expression vectors are used to insert genes of the interest into expression systems. The goal of this diploma thesis was to optimize conditions for transfection of eukaryotic cell lines CHO PF a HEK293 using of three commercially available kits. It was followed by a comparison of these transfection efficiency with standard procedures that are used in the company Generi Botech s.r.o. A plasmid pMaxGFP was used for the optimization. A cell counting in Neubaurer's chamber and a quantification of GFP in cell lysate were used to evaluate the efficiency of the transfection. Commercially available kits ...
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Definition, epidemiology and diagnosis of sepsis.
 Šplíchalová, Iva ; Jílek, Petr (advisor) ; Andrýs, Ctirad (referee)
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See also: similar author names
1 Šplíchalová, Ivana
1 Šplíchalová, Iveta
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