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National Repository of Grey Literature 2 records found  Search took 0.02 seconds. 
Role of protein NtRGS1 in cell signaling and regulation of growth of tobacco BY-2 cell line.
 Šonka, Josef ; Srba, Miroslav (advisor) ; Martinec, Jan (referee)
5 Abstract The thesis is focused on the role of regulator of G-protein signaling NtRGS1 in control of growth and cell proliferation of tobacco cell line BY-2. The protein NtRGS1 is an important candidate for being plant G-protein coupled receptor. Heterotrimeric G-proteins are involved in key signaling mechanisms in eukaryotic cells. Basic principles of this type of signaling are well conserved between plants and animals and related higher taxa. Outstanding difference of plant G-protein system is altered enzymatic activity of Gα subunit of the G-protein heterotrimer. These alterations correlate with chimeric structure and function of investigated NtRGS1 protein. The interaction of Gα and NtRGS1 is absolutely essential for running of heterotrimeric G-protein signaling in plants. Truncated versions of NtRGS1 fused to GFP were crated in the aim of protein characterization. The truncated proteins were investigated in respect of analysis of the role of NtRGS1 domains in protein targeting. Dynamic changes in NtRGS1 and selected truncated versions induced by experimental application of nutrition, especially sugars were described. Expression if Gα and NtRGS1 were investigated simultaneously. Influence of modulation of Gα and NtRGS1 expression on growth parameters of tobacco cell line BY-2 were described. Key words:...
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Heterotrimeric G-protein signaling in plants
 Šonka, Josef ; Srba, Miroslav (advisor) ; Růžička, Kamil (referee)
G-protein signalling is a key mechanism of cells interaction with the environment. It has a great significance for the coordination of cell division and differentiation in animals, Fungi and Mycetozoa. The research of this type of signalling has also a large consequence in clinical practise. This work summarizes the findings of the importance and principles of heterotrimeric G-proteins signalling in plants which have been published since the first records up to 2012.
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See also: similar author names
2 ŠONKA, Jan
2 Šonka, Jiří
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