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National Repository of Grey Literature 3 records found  Search took 0.00 seconds. 
Immunohistochemical detection of macrophages in atherosclerotic lesions brachiocephalic artery I
 Škodová, Sabina ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Sabina Škodová Immunohistochemical detection of macrophages in atherosclerotic lesions brachiocephalic artery. Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical Laboratory Technician Background: The aim of this bachelor thesis was to set imunohistochemic methods for detection of the macrophages in brachiocephalic artery apoE/LDLR receptor deficient mice. This thesis also deals with quantification of expression macrophages in group of mice which were fed with chow diet and in group of mice which were fed with low carbohydrate high protein diet. Methods: For this study were used apoE/LDLR deficient female mice which were separated into two groups (in each group were 6 mice). The first group was fed with chow diet (control group) and the second one was fed with low carbohydrate high protein diet (LCHP). This diet was administered to the mice for 8 weeks. After that the imunohistochemical analysis was performed on the cuts of brachiocephalic artery which uses Immpress method with diaminobenzidin (DAB) for better visibility of macrophages. Afterwards was performed biochemical and stereological analysis. Results: Biochemical analysis showed increased levels of total cholesterol in mice with LCHP diet. Immunohistochemical analysis showed presence of macrophages in...
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Study of the expression of some markers of endothelial function in mice with high levels of soluble endoglin.
 Škodová, Sabina ; Nachtigal, Petr (advisor) ; Blažíčková, Kateřina (referee)
Bc. Sabina Škodová Study of the expression of some markers of endothelial function in mice with high levels of soluble endoglin. Diploma thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical laboratory technician Background: The aim of this diploma thesis was to describe changes of expression eNOS and ICAM-1 in the wall of aorta of mice with low and high levels of soluble form of endoglin (Sol-Eng+). The attempt was to compare expression of both markers from the position of intensity and also from the position of area of staining. For this purpose we used immunohistochemical methods with detection in fluorescent microscope. Methods: For this study were used transgenic mice with high levels of human soluble endoglin (Sol-Eng+). Female mice were fed from their six months of age with high fat diet, which contained 1.25% of cholesterol and 40% of fat. The mice were fed with this diet for another three months. The same old female mice from the same brood with low level of soluble endoglin in plasma were used as a control group. The expression of eNOS and ICAM- 1 was defined on sections of aorta of the mice with fluorescent immunohistochemistry. Results: Immunohistochemical analysis proved weak expression of eNOS in both groups of mice, and only on vascular endothelium. The...
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Immunohistochemical detection of macrophages in atherosclerotic lesions brachiocephalic artery I
 Škodová, Sabina ; Nachtigal, Petr (advisor) ; Němečková, Ivana (referee)
Sabina Škodová Immunohistochemical detection of macrophages in atherosclerotic lesions brachiocephalic artery. Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical Laboratory Technician Background: The aim of this bachelor thesis was to set imunohistochemic methods for detection of the macrophages in brachiocephalic artery apoE/LDLR receptor deficient mice. This thesis also deals with quantification of expression macrophages in group of mice which were fed with chow diet and in group of mice which were fed with low carbohydrate high protein diet. Methods: For this study were used apoE/LDLR deficient female mice which were separated into two groups (in each group were 6 mice). The first group was fed with chow diet (control group) and the second one was fed with low carbohydrate high protein diet (LCHP). This diet was administered to the mice for 8 weeks. After that the imunohistochemical analysis was performed on the cuts of brachiocephalic artery which uses Immpress method with diaminobenzidin (DAB) for better visibility of macrophages. Afterwards was performed biochemical and stereological analysis. Results: Biochemical analysis showed increased levels of total cholesterol in mice with LCHP diet. Immunohistochemical analysis showed presence of macrophages in...
Detailed record

See also: similar author names
1 Škodová, Svatava
2 Škodová, Šárka
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