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Study of the role of nucleoside transporters in intestinal absorption with the use of in vitro transport method based on the Caco-2 cell line.
Ďurišová, Markéta ; Červený, Lukáš (advisor) ; Hofman, Jakub (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Mgr. Markéta Ďurišová Supervisor: PharmDr. Lukáš Červený, Ph.D. Title of rigorosum thesis: Study of the role of nucleoside transporters in intestinal absorption with the use of in vitro transport method based on the Caco-2 cell line. Perorally used drugs are primarly absorbed in the small intestine. Enterocytes, forming the intestinal barrier, express a large number of transporters, representing both ATP-binding (ABC) and "solute carrier" (SLC) transporters, which can significantly affect the pharmacokinetics of the drugs transmitted by them. For this reason, FDA and EMA are currently focusing on studying these interactions and their effects on drug permeability. The aim of this thesis was to investigate whether the equilibrative nucleoside transporters (ENTs), belonging to the SLC transporters, are involved in the absorption of nucleosides (adenosine and thymidine) and subsequently abacavir, the nucleoside antiretroviral. The in vitro transport method was performed on polarized, Caco-2 cell monolayer. By the statistical analysis of acquired data was found, that although the expression of ENTs was confirmed on the apical side of the Caco-2 cell membrane, the transport of nucleosides [3H]ADE and...
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Study of the role of nucleoside transporters in intestinal absorption with the use of in vitro transport method based on the Caco-2 cell line.
Ďurišová, Markéta ; Červený, Lukáš (advisor) ; Hofman, Jakub (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmacology & Toxicology Student: Mgr. Markéta Ďurišová Supervisor: PharmDr. Lukáš Červený, Ph.D. Title of rigorosum thesis: Study of the role of nucleoside transporters in intestinal absorption with the use of in vitro transport method based on the Caco-2 cell line. Perorally used drugs are primarly absorbed in the small intestine. Enterocytes, forming the intestinal barrier, express a large number of transporters, representing both ATP-binding (ABC) and "solute carrier" (SLC) transporters, which can significantly affect the pharmacokinetics of the drugs transmitted by them. For this reason, FDA and EMA are currently focusing on studying these interactions and their effects on drug permeability. The aim of this thesis was to investigate whether the equilibrative nucleoside transporters (ENTs), belonging to the SLC transporters, are involved in the absorption of nucleosides (adenosine and thymidine) and subsequently abacavir, the nucleoside antiretroviral. The in vitro transport method was performed on polarized, Caco-2 cell monolayer. By the statistical analysis of acquired data was found, that although the expression of ENTs was confirmed on the apical side of the Caco-2 cell membrane, the transport of nucleosides [3H]ADE and...
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The implementation of the International Health Regulations (2005) in the Czech Republic
ĎURIŠOVÁ, Markéta
This diploma thesis on the theme:"The implementation of the International Health Regulations, 2005 in Czech Republic.", is divided into theoretical and practical part.The theoretical part focuses on the International Health Regulations 2005 capacity required, and the measures proposed in the Czech Republic. It describes the history of the International Health Regulations and the implementation of International Health Regulations 2005, in Czech Republic.I also deal, in this part of thesis, about highly contagious diseases.Data processing research for this study was collected in the district of Český Krumlov. The research was conducted by a qualitative method.The sample consisted of 8 respondents.The aim of the study was to determine whether practitioners know how to proceed in case of a patient with a highly contagious disease in their office.This diploma thesis could serve as a source of information, whether the Czech Republic meet the requirements formulated by the World Health Organization in the International Health Regulations 2005.
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Prevention of influenza in persons aged over 65 years and in persons with high-risk medical conditions in the district of Český Krumlov and their attitude toward vaccination.
ĎURIŠOVÁ, Markéta
The topic of my thesis is the issue of prevention of influenza in persons aged 65 years and persons with high-risk medical conditions in the district of Český Krumlov and their attitude toward vaccination. The study is divided into two parts, theoretical and practical. In the theoretical part a comprehensive knowledge of epidemiology, the cause, treatment and opportunities for the prevention of influenza are mentioned. The practical part is focused on a vaccination coverage survey and opinions on vaccination in people aged over 65 years and persons with high-risk medical conditions in the district of Český Krumlov and analysis of results obtained by the research investigation. The purpose of the study is to analyze the reasons that lead to small vaccination coverage of these groups and to find out how to increase vaccination rates. To obtain the data I used the method of quantitative research by means of anonymous questionnaires. A total of 300 questionnaires were distributed. The final number for data processing was 190 questionnaires. In connection with the study I set out four hypotheses. The hypotheses were confirmed or refuted on the basis of my investigation assessment. The research results have shown that the most frequent reason for non-vaccination is the lack of information the respondents have about influenza disease and influenza vaccination. This implies the fact that the most respondents do not believe in the effects of vaccination, and many people do not perceive influenza as a serious condition. Therefore, through this study I would like to emphasize the importance of information that can help people consider the risk of influenza disease and encourage them to use vaccination.
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