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Attention bias toward copulatory movement: An eyetracking study
Čihařová, Barbora ; Bártová, Klára (advisor) ; Hejtmánek, Lukáš (referee)
Pozornostní zkreslení vůči kopulačnímu pohybu: Eyetrackingová studie Abstract The diploma thesis is a part of the project Investigation of male and female sexual reactivity: psychophysiological and subjective responses to videostimuli of the Faculty of Humanities, UK (GA20-03604S). The eyetracking study represents one of three parts of the research and focuses on the level of attention given to non/copulatory sexual movement. Studies examining sexual arousal indicate that both men and women react differently when exposed to various erotic stimuli. For example, men exhibit higher arousal to preferred stimuli directly related to their sexual orientation, while women respond even to subjectively non-preferred stimuli. Researchers explain this phenomenon as a possible sexual response to copulatory movement, where non/preferred stimuli play a less significant role in female arousal than in male arousal. The aim of this research was to test the level of attention towards non/copulatory sexual movement using an eyetracking device. We analyzed eye gazes in both sexes, with the goal of determining whether attention to different videos (copulatory, non-copulatory) differs between heterosexual men and women. The analysis of the resulting measurements did not reveal any significant effects in terms of attentional...
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Effect of polyhistidine modification of viral particles on their intracellular localization and gene delivery to the nucleus
Číhařová, Barbora ; Španielová, Hana (advisor) ; Grantz Šašková, Klára (referee)
Viral vectors derived from mouse polyomavirus are a convenient tool for studying the targeted delivery of therapeutical agents into the cells and cellular organelles. Vectors derived from mouse polyomavirus face difficulties similar to other nanoparticles, as they often end up trapped inside an endosome where they are subsequently degraded. This diploma explored the potential of vector modifications, which have the potential to make the transport to the nucleus or cytosol more effective. This work had particularly focused on increasing the transduction efficiency by modifying particle's internally localized VP3 capsid protein with covalently bound membrane-penetrating peptides. Primary covalent genetic modification to the VP3 protein was the polyhistidine peptide KH27K. Its potential of improving the transduction effectivity was compared with two other peptide modifications - LAH4 and R8. The results of the transduction test showed that covalently bound R8 peptide had many-fold improved the transport to the nucleus when compared to the unmodified particles. The modification with LAH4 peptide had been regarded more effective only when was associated with the particles non-covalently. In such scenario the transduction efficiency rose 40-times when compared with unmodified particles. Polyhistidine...
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Influence of size and geometry of nanoparticles on cellular internalization pathways
Číhařová, Barbora ; Španielová, Hana (advisor) ; Lišková, Petra (referee)
Nanoparticles can be used in biomedical disciplines as carriers for transport of diagnostic as well as therapeutic substances into cells. Variety of different shapes, sizes and different compositions are used experimentally. Despite the discoveries already made in this area, the exact nature of the interaction between a nanoparticle and a cell has not been fully understood yet. The objective of this thesis is to provide the knowledge about possibilities of utilisation and aspects influencing the interaction between the cell membrane and several types of nanoparticles: liposomes, gold nanoparticles and virus-like nanoparticles. The comparison shows that generalisation of the mechanism of nanoparticle entry into the cell is problematic, although it seems that the spherical nanoparticles with the diameter of 50 nm provide the most efficient entry.
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