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Molecular basis of interactions between Dishevelled 3 (Dvl3) and Protein Regulator Of Cytokinesis 1 (PRC1)
Kropáčková, Veronika ; Bařinka, Cyril (advisor) ; Macůrková, Marie (referee)
Scaffolding protein Disheveled (Dvl) is a key component of Wnt signaling cascades. Dvl participates in a number of biological processes, such as cell proliferation, differentiation and migration, determination of cell polarity, and also stem cell self-renewal. It is therefore indispensable for the correct embryo development and tissue homeostasis in adulthood. The protein regulator of cytokinesis (PRC1) is a microtubule-associated protein. PRC1 is involved in spindle midzone formation during cell division. Spindle midzone precedes the contractile ring assembly and is essential for normal cell cleavage. In our laboratory, PRC1 was identified as a putative interaction partner of DVL3. This master thesis is focused on delineation of the interaction interface between DVL3 and PRC1 using TIRF microscopy (Total Internal Reflection Fluorescence microscopy). To this end, full-length DVL and PRC1 proteins together with their truncated variants were designed, expressed and purified. It was discovered that PRC1 interacts with all three DVL isoforms and the N-terminal part of PRC1 is required for the interaction between PRC1 and DVL3. Furthermore, the DEP domain of DVL3 is likely involved in PRC1interactions. Key words: Dishevelled 3, DVL3, Protein regulator of cytokinesis 1, PRC1, interaction interface, TIRF...
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Molecular basis of interactions between Dishevelled 3 (Dvl3) and Protein Regulator Of Cytokinesis 1 (PRC1)
Kropáčková, Veronika ; Bařinka, Cyril (advisor) ; Macůrková, Marie (referee)
Scaffolding protein Disheveled (Dvl) is a key component of Wnt signaling cascades. Dvl participates in a number of biological processes, such as cell proliferation, differentiation and migration, determination of cell polarity, and also stem cell self-renewal. It is therefore indispensable for the correct embryo development and tissue homeostasis in adulthood. The protein regulator of cytokinesis (PRC1) is a microtubule-associated protein. PRC1 is involved in spindle midzone formation during cell division. Spindle midzone precedes the contractile ring assembly and is essential for normal cell cleavage. In our laboratory, PRC1 was identified as a putative interaction partner of DVL3. This master thesis is focused on delineation of the interaction interface between DVL3 and PRC1 using TIRF microscopy (Total Internal Reflection Fluorescence microscopy). To this end, full-length DVL and PRC1 proteins together with their truncated variants were designed, expressed and purified. It was discovered that PRC1 interacts with all three DVL isoforms and the N-terminal part of PRC1 is required for the interaction between PRC1 and DVL3. Furthermore, the DEP domain of DVL3 is likely involved in PRC1interactions. Key words: Dishevelled 3, DVL3, Protein regulator of cytokinesis 1, PRC1, interaction interface, TIRF...
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Tubulin post-translational modifications
Kropáčková, Veronika ; Bařinka, Cyril (advisor) ; Dostál, Vojtěch (referee)
Microtubules composed of αβ-tubulin heterodimers are an integral part of the cellular cytoskeleton of eukaryotic organisms. They participate in the cellular transport, determine the distribution of membrane organelles and help defining cellular polarity. Microtubules are part of dynamic structures such as mitotic spindle, but they also form stable structures such as flagellar and ciliar axonemes. Microtubules have many distinct functions in cells and tissues and therefore must differ from each other in some way. Post-translational modificationsof αβ-tubulin in microtubules are the major source of their diversity and collectively define so-called tubulin code. Twelve posttranslational modifications of tubulin/microtubules have been identified so far. Unraveling the mechanisms involved in post-translational modifications of tubulin/microtubules, including the identification of responsible enzymes, is an important source of understanding of the effects of these modifications on biological functions. Key words: tubulin, post-translational modificaton, microtubules, acetylation, tyrosination, polyamination, polyglutamylation
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Možnosti zapojení zemědělského podniku do komoditní vertikály mléka
Kropáčková, Veronika
This thesis focuses on characteristic changes caused by expansion of agribusiness and some shaping specifics of Czech farms and business environment which were caused by the transformation processes and the Czech Republic's accession to the European Union. The thesis also describes the developement of milk commodity chain in the Czech Republic, discusses the farm status in it and also fundamental factors determining the success of primary production. Based on the results, the thesis also deals with the elimination of dependence of one specific farm on followup subjects. The main part of the thesis is a business plan of independent processing of farm milk production. The results show that independent processing of farm production is one of the suitable possibilities nowadays, how to keep the viability of small and medium sized farms.
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Modelování tržeb v kavárenství
Kropáčková, Veronika
The aim of this thesis is based on modelling of revenue streams in a sweatshop Klokánek. At the beginning the reader will get familiar with basic terminology and methods related to marketing analysis and time series forecasting that will be later on applied in the practical part using historical business data. The aim is to create quantitative basis for pricing and estimation of revenues for a selected product, partly based on competing sweatshops pricing and quality of services. The particular forecasting was conducted for several variants of sales levels: Holt-Winters method, Monte Carlo simulation, SARIMA. These inputs were collected for the recommendations that were provided in the end of the thesis.
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Agroturistické využití ekofarmy rodiny Kropáčkových v Nelepči
Kropáčková, Veronika
This bachelor thesis looks into the possibility of the agrotouristic use of the organic family farm in Nepeleč. It provides a general characterization of organic agriculture and informs about controlling and certifitation organizations of organic farming. Based on the current farm-state evaluation, the SWOT analysis is made and some strategies are suggested. The organic farm's independence of milk purchaser - the ability of independent milk processing with the possibility of making use of its own electric energy (photovoltaic power x biogas plant) is described here. Mentioned options of the farm's growth and their asset are compared in the last part.
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