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Bacterial adhesion to surfaces
Budil, Jakub ; Beranová, Jana (advisor) ; Kuthan, Martin (referee)
Bacterial adhesion is a process of attachment of a planktonic cell to the surface and the necessary step for further biofilm formation. Most bacterial species are capable of biofilm formation. Bacteria within biofilm exhibit increased resistance to antibiotics and other external factors, which makes the eradication of adhered bacteria rather difficult. Bacterial adhesion is very complex process affected by many physical and chemical factors as well as by the adhesive molecules present on the substrate and cell surface. Accurate description of these factors enables to design appropriate modifications of both the substrate and the environment with the aim to fabricate the antiadhesive and antibacterial surfaces or to increase the efficiency in bacteria-based biotechnological processes. Whereas in medical applications the main goal is to decrease bacterial adhesion, the increased adhesion is desirable in certain processes employing bacteria, as is for example the wastewater treatment. This thesis reviews main theories used for prediction of bacterial adhesion, describes the process of adhesion and physical, chemical and biological factors that influence it. It also contains an overview of antiadhesive and antibacterial surfaces. Key words: Bacteria, adhesion, pili, flagellum, antiadhesive and...
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Antibacterial and antiadhesive properties of carbon nanomaterials
Budil, Jakub ; Lišková, Petra (advisor) ; Zikánová, Blanka (referee)
Increasing interest in industrial and medical applications of carbon nanomaterial leads to the need to examine its interactions with living systems. Nanocrystalline diamond (NCD) films possess high mechanical and chemical stability which, together with its biocompatibility with human cells, enables applications in human body. Some of carbon nanoparticles possess strong antibacterial activity. In this work the effects of NCD with hydrogen, oxygen and fluorine termination deposited on glass and silicone on adhesion of gram-negative bacteria Escherichia coli K-12 in mineral medium is described and the impact of cultivation medium on effects of NCD films is compared. Prior the growth of the E. coli biofilm on NCD films, the method for quantification of biofilm using crystal violet staining and the method for biofilm cultivation in mineral medium were optimised. The properties of NCD film are independent on the base substrate. Hydrogen and fluorine terminated NCD films show antiadhesive properties only in mineral medium but not in complex medium. This is explained by formation of a conditioning film on the surface of the NCD film during cultivation in complex medium. On the other hand, O-NCD film supports bacterial adhesion in both cultivation media. Second part of this thesis is dedicated to carbon...
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Bacterial adhesion to surfaces
Budil, Jakub ; Beranová, Jana (advisor) ; Kuthan, Martin (referee)
Bacterial adhesion is a process of attachment of a planktonic cell to the surface and the necessary step for further biofilm formation. Most bacterial species are capable of biofilm formation. Bacteria within biofilm exhibit increased resistance to antibiotics and other external factors, which makes the eradication of adhered bacteria rather difficult. Bacterial adhesion is very complex process affected by many physical and chemical factors as well as by the adhesive molecules present on the substrate and cell surface. Accurate description of these factors enables to design appropriate modifications of both the substrate and the environment with the aim to fabricate the antiadhesive and antibacterial surfaces or to increase the efficiency in bacteria-based biotechnological processes. Whereas in medical applications the main goal is to decrease bacterial adhesion, the increased adhesion is desirable in certain processes employing bacteria, as is for example the wastewater treatment. This thesis reviews main theories used for prediction of bacterial adhesion, describes the process of adhesion and physical, chemical and biological factors that influence it. It also contains an overview of antiadhesive and antibacterial surfaces. Key words: Bacteria, adhesion, pili, flagellum, antiadhesive and...
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