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Simulation of adhesion and transmigration imune-cells through capillary wall
Morgaenko, Katsiarina ; Čmiel, Vratislav (referee) ; Chmelíková, Larisa (advisor)
Teoretická část této práce obsahuje biofyzikální popis endoteliální vrstvy a transmigrace buněk přes tuto vrstvu. Dále popisuje charakteristiky všech důležitých komponent pro vytvoření modelu cévy in vitro (endoteliální buňky, kmenové buňky, leukocyty, Calcein AM, PKH26). Praktická část práce je věnována sestavení in-vitro modelů dvojrozměrných a trojrozměrných endoteliálních vrstev, jejich zobrazení pomoci mikroskopu a testování interakce leukocytů a kmenových buněk s těmito endoteliálními vrstvami. V závěru je navrhnut nejlepší tvar a velikost mikrofluidních systémů simulujících kapiláry.
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Foreknowledge of women in childbed about the possibility of navel cord blood taking
BLÁHOVÁ, Martina
The objective of the thesis was to determine the level of expectant mothers' awareness of the possibility of umbilical cord blood collection. Umbilical cord blood can be collected only at one moment {--} after the child has been delivered. Umbilical cord blood comprises stem cells that cannot be found in the body any other time in human life. Stem cells have been used to treat blood diseases for several years. Umbilical cord blood is contained in umbilical cord and placenta. Umbilical cord blood collection is not dangerous under any circumstances and donation is entirely optional. The midwife is a member of the team that participates in umbilical cord blood collection. She is familiar with the correct blood collection technique. She educates pregnant women during pregnancy and also during childbirth about the possibility of umbilical cord blood collection and use. In spite of the fact that pregnant women have enough information about the possibility of umbilical cord blood collection, it is necessary to improve awareness of the importance of the collected umbilical cord blood - e.g. in prenatal courses or by creating a complex educative material. Nowadays umbilical cord blood is used mainly in the treatment of children's haemoplastic diseases, but in the future usage of stem cells could exceed the imagination. The advantage is that umbilical cord blood can be collected safely and painlessly after the childbirth and it can be stored in the long term in the umbilical cord blood banks. Umbilical cord blood preservation may become a meaningful health and life policy.
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Cell fusion and carcinogenesis
MARŠÍKOVÁ, Hana
The origin of cancer remains enigmatic. The object of this study was to find out the impact of BMDCs fusion without any carcinogens on cancer progression. The arising instability of genome could give rise to malignancy.
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