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Národní úložiště šedé literatury Nalezeno 2 záznamů.  Hledání trvalo 0.00 vteřin. 
Nanoporous Enzyme-Based Impedimetric Biosensor
 Košelová, Z.
The ordered nanopores surfaces has been studied in the field of biosensors for the detection of affinity interactions such as antigen-antibody or nucleic acid hybridization. Nanopores are frequently fabricated by anodic oxidation of aluminium. This method is practically simple method of highly ordered pore formation, but is not suitable for massive production. The principle of detection is usually based on the degree of pore blockage by biomolecular interaction that allows a sensitive electrochemical response. Here we show the development and characterization of the nanopore-based enzymatic biosensor for the detection of analytes. We performed and tested the concept of the simple and versatile method of nanopores formation on the electrode using non-conductive polystyrene nanoparticles. The variability in pore size was modulated by nanoparticles diameter (40 and 80 nm). We measured response of our designed biosensor using electrochemical impedance spectroscopy in presence of redox mediator at low potential. The smaller diameter of nanopores increases charge transfer resistance. Biosensors with 80 nm nanoparticles had behaviour similar to reference without nanoparticles, therefore the pore was too wide for this design. The limit of detection from Rct showed to be at most 0.01 mM for every sample type. This proposed biosensor contributes to the basic knowledge about nanopores sensors, and it could have a potential, for example, in the development of ―point of care‖ devices.
Úplný záznam
Effect of Adsorbable Foreign Gas on Evaporation of water from Porous Aerosol Particles
 Levdansky, V.V. ; Smolík, Jiří ; Ždímal, Vladimír
Adsorption of molecules of a foreign gas on the surface of nanoscale objects can influence phase transitions in the heterogeneous systems with nanoobjects. Some problems related to adsorption of foreign molecules on the surface of nanoscale particles (clusters) were considered in (Luijten et al., 1997; Levdansky et al., 2006). Here we discuss the effect of the adsorbable foreign gas on water evaporation from porous aerosol particles with nanoscale pores.
Plný tet: SKMBT_C22013101814593 - Stáhnout plný textPDF
Plný text: content.csg - Stáhnout plný textPDF
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