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The first experiencies with new exposure system for the whole body inhalation experiments with small animals
Večeřa, Zbyněk ; Mikuška, Pavel ; Moravec, Pavel ; Smolík, Jiří
The first experiences with an inhalation chamber constructed for long-term exposure studies of small animals to nanoparticles under strictly controlled experimental conditions. The inhalation chamber was build from a glass and stainless steel and allows possible long-term inhalation procedures with up to four discrete groups of mice or of rats population under controlled illumination, temperature, relative humidity and pressure. Doses up to 1 x 10(6) particles/cm(3) and size (10-60 nm) of nano-sized particles were synthesized via aerosol route in a hot wall tube flow reactor using thermal decomposition of metal organic precursors. The function of exposure system for whole body inhalation experiments was studied under real conditions in period from May to September 2012. No animals were used within this trial experiment.
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Unique exposure system for the whole body inhalation experiments with small animals
Večeřa, Zbyněk ; Mikuška, Pavel ; Moravec, Pavel ; Smolík, Jiří
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of inhalated nano-sized particles (Pb, Cd) and their allocation in organs“. The inhalation chamber builds from a glass and stainless steel and makes possible long-term inhalation procedures (up to 60 days, 24 hours/day) with up to four discrete groups of mice population (up to 60 individuals each) or of rats population (up to 20 individuals each) under controlled illumination, temperature, relative humidity, doses (0.5 x 106 - 5 x 106 particles/cm3) and size (10-60 nm) of nano-sized particles which are synthesized via aerosol route in a hot wall tube flow reactor using metal organic precursors.
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První zkušenosti s inhalací nanočástic malými zvířaty
Mikuška, Pavel ; Večeřa, Zbyněk
Příspěvek popisuje inhalační komoru konstruovanou pro dlouhodobé inhalační studie malých zvířat nanočásticemi za přísně kontrolovaných podmínek (tj., teplota, relativní vlhkost, světlo, tlak). Nanočástice byly syntetizovány on-line v trubkovém průtokovém reaktoru z kovových organických prekursorů. Experimenty potvrdily, že inhalační komora je vhodná pro „whole body“ experimenty s malými zvířaty.
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Unique exposure system for the whole body inhalation experiments with small animals
Večeřa, Zbyněk ; Mikuška, Pavel ; Dočekal, Bohumil ; Vojta, Šimon ; Míšek, Ivan ; Moravec, Pavel ; Smolík, Jiří
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of inhalated nano-sized particles (Pb, Cd) and their allocation in organs“. The inhalation chamber builds from a glass and stainless steel and makes possible long-term inhalation procedures (up to 60 days, 24 hours/day) with up to four discrete groups of mice population (up to 60 individuals each) or of rats population (up to 20 individuals each) under controlled illumination, temperature, relative humidity, doses (0.5 x 106 - 5 x 106 particles/cm3) and size (10-60 nm) of nano-sized particles which are synthesized via aerosol route in a hot wall tube flow reactor using metal organic precursors.
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