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Biotechnological conversion of spent brewery grains into microbial polymers
 Bystrická, Klaudia ; Nováčková, Ivana (oponent) ; Obruča, Stanislav (vedoucí práce)
The presented bachelor thesis deals with the study of biotechnological production of polyhydroxyalkoanates (PHA), using hydrolysates from brewer’s spelt grain (BSG) and selected bacterial strains Schlegelella, Halomonas, Tepidimonas and Burkholderia. The thesis aims to determine whether it is possible to use BSG hydrolysates as a substrate for PHA production, with the emphasis on different pretreatment methods of the lignocellulosic material. BSG was submitted to quantitative analysis and the optimum temperature and pH of the commercial enzymatic cocktail used for the hydrolysation was determined. The most effective transformation of sugars to PHA was accomplished by bacteria H. organivorans with the use of a hydrolysate from plasma pretreated BSG. Hydrolysates which were prepared with moderate methods, contained less bacterial inhibitors and sufficient amounts of sugars. These hydrolysates served therefore as preferable substrates for the microorganism growth.
Úplný záznam
Biotechnological conversion of spent brewery grains into microbial polymers
 Bystrická, Klaudia ; Nováčková, Ivana (oponent) ; Obruča, Stanislav (vedoucí práce)
The presented bachelor thesis deals with the study of biotechnological production of polyhydroxyalkoanates (PHA), using hydrolysates from brewer’s spelt grain (BSG) and selected bacterial strains Schlegelella, Halomonas, Tepidimonas and Burkholderia. The thesis aims to determine whether it is possible to use BSG hydrolysates as a substrate for PHA production, with the emphasis on different pretreatment methods of the lignocellulosic material. BSG was submitted to quantitative analysis and the optimum temperature and pH of the commercial enzymatic cocktail used for the hydrolysation was determined. The most effective transformation of sugars to PHA was accomplished by bacteria H. organivorans with the use of a hydrolysate from plasma pretreated BSG. Hydrolysates which were prepared with moderate methods, contained less bacterial inhibitors and sufficient amounts of sugars. These hydrolysates served therefore as preferable substrates for the microorganism growth.
Úplný záznam

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