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Use of yeasts of the genus Metschnikowia for the lipid production
Švitková, Bibiána ; Márová, Ivana (oponent) ; Němcová, Andrea (vedoucí práce)
The oleaginous yeasts have an ability to accumulate an increased number of lipids, under certain circumstances. These microbial lipids differentiate in the number of fat acids present, which enables their wide application in biotechnological industry. This master’s thesis is aimed on lipid production, number of the fat acid groups present, and squalene production by Metschnikowia yeasts, based on the cultivating conditions. Biomass and lipid production was observed in separate cultivation media, with the addition of the different waste substrates. Production properties were observed by method of the gas chromatography. For the squalene production observation, a HLPC method was chosen. All production groups were able to accumulate lipids on the waste substrate, although in different values. These values were very individual, especially in the areas of the specific groups and growth on the given substrate. The lipid composition was different, which was caused by differences in the waste substrates. With regards to the squalene production – the yeasts from the Metschnikowia family were not able to produce squalene in the presence of the terbinafine and its increasing concentration. Therefore, the same procedure was chosen, as it was for the Yarrowia lipolytica yeast, with the difference in the sterol synthesis, however squalene was still not produced this way.
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Use of yeasts of the genus Metschnikowia for the lipid production
Švitková, Bibiána ; Márová, Ivana (oponent) ; Němcová, Andrea (vedoucí práce)
The oleaginous yeasts have an ability to accumulate an increased number of lipids, under certain circumstances. These microbial lipids differentiate in the number of fat acids present, which enables their wide application in biotechnological industry. This master’s thesis is aimed on lipid production, number of the fat acid groups present, and squalene production by Metschnikowia yeasts, based on the cultivating conditions. Biomass and lipid production was observed in separate cultivation media, with the addition of the different waste substrates. Production properties were observed by method of the gas chromatography. For the squalene production observation, a HLPC method was chosen. All production groups were able to accumulate lipids on the waste substrate, although in different values. These values were very individual, especially in the areas of the specific groups and growth on the given substrate. The lipid composition was different, which was caused by differences in the waste substrates. With regards to the squalene production – the yeasts from the Metschnikowia family were not able to produce squalene in the presence of the terbinafine and its increasing concentration. Therefore, the same procedure was chosen, as it was for the Yarrowia lipolytica yeast, with the difference in the sterol synthesis, however squalene was still not produced this way.
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Detekce apikulátních a ušlechtilých kvasinek v kvasícím moštu pomocí PCR
Kosek, Filip
V rámci této diplomové práce bylo zkoumáno, jak moc ovlivňuje charakter vína apiku-látní kvasinka Metschnikowia pulcherrima. Za tímto účelem byla vyrobena dvě vína z odrůdy Ryzlink vlašský stejným technologickým postupem s tím rozdílem, že do dvou oddělených moštů byl přidán zákvas s odlišnými kvasinkami. Literární část práce pojednává obecně o kvasinkách, které jsou používány ve vinařství. Popsány jsou jak apikulátní, tak i ušlechtilé kvasinky. V této části je také roz-vedena problematika polymerázové řetězové reakce a jí podobné metody. Metodika se zaobírá možnostmi izolace DNA kvasinek rodu Metschnikowia pulcherrima z kvasícího moštu a následnou kvantifikací kvasinek pomocí metody Real-time PCR. Z hodnocení a porovnání vín, kterého se zúčastnili laici i odborná veřejnost, vyplynulo, že kvasinka ovlivňuje zásadním způsobem charakter vína.
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