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Study of plasma activated water influence on soil microorganisms
Poláková, Marianna ; Dvořáčková,, Helena (oponent) ; Krčma, František (vedoucí práce)
Bachelor thesis is focused on the impact of plasma activated water on soil microorganisms. Reactive spieces of oxygen and nitrogen are present in plasma activated water, which are responsible for its biological and chemical properties. Thanks to its unique features, plasma activated water is used for therapeutic purpose or in agriculture. The theoretical part is concentrated on general information about soil, its properties and microorganisms found in it. Furthermore, it deals with basic information about plasma and sources used for its production. The last part presents the plasma activated water and its specific characteristics. Experimental part deals with the application of distilled water, plasma activated water and a mixture of distilled and plasma activated water on soil. In the first set of experiments, it was an application directly on the soil, the application to the soil extract was in the second set. Bacteria were inoculated on culture medium in concentrations 10-2, 10-4 and 10-6. The number of formed colonies was observed and then some microorganisms has been identified. Based on the results, it can be stated that plasma activated water had a positive effect on the microorganism colonies growth. However, the subject of further work will be to determine whether it is positive for pathogenic microorganisms or not.
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Study of plasma activated water application on the total soil microbial biomass content
Poláková, Marianna ; Zlámalová Gargošová, Helena (oponent) ; Krčma, František (vedoucí práce)
The aim of the diploma thesis was to choose the optimal method for evaluating the content of total microbial biomass in the soil, using plasma-activated water as irrigation, based on literature research. For this purpose, the fumigation-extraction method was chosen, which was evaluated using TOC/TN. The work further focused on the characterization of the physicalchemical properties of plasma-activated water and soil. The rate of decomposition of organic matter in the soil was monitored using the tea bag method, where green and roiboos tea are used to study the rate of decomposition. The content of total water-extractable polyphenolic substances was also determined in green tea. Based on cultivation tests, the presence of soil microorganisms was determined. From the measured data, it was found that the plasmaactivated water does not have a negative effect on the pH of the soil. However, probably as a result of its application, the values of the total microbial biomass in plasma-activated water samples for 10 minutes decreased to the value of (8063±1900) mgkg-1 for TOC and (98±74) mgkg-1 for TN compared to the distilled infusion water, where TOC values were (18068±4186) mgkg-1 and TN (123±10) mgkg-1. .
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Study of plasma activated water influence on soil microorganisms
Poláková, Marianna ; Dvořáčková,, Helena (oponent) ; Krčma, František (vedoucí práce)
Bachelor thesis is focused on the impact of plasma activated water on soil microorganisms. Reactive spieces of oxygen and nitrogen are present in plasma activated water, which are responsible for its biological and chemical properties. Thanks to its unique features, plasma activated water is used for therapeutic purpose or in agriculture. The theoretical part is concentrated on general information about soil, its properties and microorganisms found in it. Furthermore, it deals with basic information about plasma and sources used for its production. The last part presents the plasma activated water and its specific characteristics. Experimental part deals with the application of distilled water, plasma activated water and a mixture of distilled and plasma activated water on soil. In the first set of experiments, it was an application directly on the soil, the application to the soil extract was in the second set. Bacteria were inoculated on culture medium in concentrations 10-2, 10-4 and 10-6. The number of formed colonies was observed and then some microorganisms has been identified. Based on the results, it can be stated that plasma activated water had a positive effect on the microorganism colonies growth. However, the subject of further work will be to determine whether it is positive for pathogenic microorganisms or not.
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