Horká, Marie - Výsledky hledání - Digitální repozitář

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	Zeleň kolem silničních komunikací Horká, Marie Úplný záznam
	Metodický postup čištění virů pomocí polyethylenglykolu (PEG) a sacharózového gradientu Kubíček, O. ; Kubíčková, Z. ; Rosenbergová, K. ; Horká, Marie Prvním krokem je odstranění větších nečistot, baktérií a buněk první centrifugací. poté je k supernatantu obsahujícímu virus přidán PEG a NaCl. Působením PEG a iontové síly NaCl dojde k vysrážení viru, který následně sedimentuje při centrifugaci již při zrychlení okolo 10000g. Na sacharózovém gradientu dojde k vyčištění viru od nečistot odlišné hustoty. Úplný záznam
	Separation of clinically significant microorganisms from CSF by CIEF with UV detection and MALDI-TOF MS Horká, Marie ; Šalplachta, Jiří ; Kubesová, Anna ; Růžička, F. ; Šlais, Karel The identification of most of the clinically significant species by the conventional laboratory methods is still time-consuming and often insufficient for ensuring early targeted therapy. This is especially important in the case of the nosocomial infections where the number of microorganisms in the cerebrospinal fluid is often ranging from units up to hundreds of cells per milliliter during bacterial infections of the central nervous system. The electrophoretic techniques with UV-detection reach the detection limit of intact cells approximately from 107 cells mL-1. Úplný záznam
	Silica-based monolithic capillary columns modified to zwitterionic stationary phase for hydrophilic interaction liquid chromatography Moravcová, Dana ; Planeta, Josef ; Kahle, Vladislav ; Horká, Marie ; Roth, Michal Zwitterionic monolithic capillary columns intended for isocratic gradient hydrophilic interaction chromatography (HILIC) separations are introduced. Silica-based capillary columns (150 mm x 0.1 mm) were prepared by acidic hydrolysis of tetramethoxysilane in the presence of polyethylene glycol and urea. The modification by a 3-(trimethoxysilyl)propyl methacrylate and then by a zwitterionic [2-(methacryloyloxy)ethyl]-dimethyl-(3-sulfopropyl)-ammonium hydroxide to HILIC stationary phase bearing sulfoalkylbetaine groups on its surface followed. Prepared columns were characterized in HILIC separation mode employing mobile phase containing 10% (v/v) of 5 mM ammonium acetate pH = 4.5 in acetonitrile. Comparison with the commercially available ZIC-HILIC® column (Merck SeQuant®) under the same separation conditions using a mixture of aromatic carboxylic acids as a sample was done on the basis of separation efficiency of tested columns as well as retention factors and peak asymmetry of individual solutes. Úplný záznam
	Filtration microcartridge and capillary isoelectric focusing for the analysis low number of microorganisms in real samples Kubesová, Anna ; Horká, Marie ; Šalplachta, Jiří ; Horký, J. At present, detection and identification of microorganisms in real biological samples needs very sensitive methods for correct diagnosis and therapy in medical practice, biotechnology, agriculture, food safety and quality etc. Accordingly, it is essential to develop new cheap techniques for pathogen identification, particularly for pathogen in complex biological samples. Pre-concentration, separation, and sensitive detection of whole cells in one step are great potential and advantage for detection of pathogens in low concentration. Combination of filtration microcartridge and capillary isoelectric focusing was developed for pre-concentration and pre-separation of microorganisms from real suspensions and the possibility its application to real samples was verified. Úplný záznam
	Differences in fingerprints of biofilm-positive and biofilm-negative Candida strains exploitable for clinical practice Vykydalová, Marie ; Horká, Marie ; Šalplachta, Jiří ; Růžička, E. ; Kahle, Vladislav In this study, we have presented differences in fingerprints of Candida parapsilosis and Candida metapsilosis as well as their biofilm-positive and biofilm-negative forms. Fingerprints of yeasts were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis, capillary isoelectric focusing, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Samples for analyses were prepared by treatment of yeasts by boiling water or ethanol. Fingerprints of tested yeasts cells have differed in all cases. This knowledge can be used for obtaining rapid and simple method which distinguishes above mentioned yeasts in standard biochemical laboratories. Úplný záznam
	MALDI-TOF MS of Dickeya and Pectobacterium species - Characterization of plant pathogenic bacteria Šalplachta, Jiří ; Kubesová, Anna ; Horký, J. ; Horká, Marie MALDI-TOF MS was applied to reliable characterization of Dickeya and Pectobacterium species in this study. Our results confirm suitability of suggested analytical approach for efficient bacterial differentiation of species based on species specific mass fingerprints. MALDI-TOF MS of intact bacterial cells require minimum sample handling and is less time-consuming compared to conventional methods. Úplný záznam
	Sub-, near- and supercritical water in analytical separations and instrumentation development Karásek, Pavel ; Šťavíková, Lenka ; Hohnová, Barbora ; Planeta, Josef ; Moravcová, Dana ; Horká, Marie ; Šlais, Karel ; Roth, Michal Applications and prospects of subcritical and supercritical water in analytical separations and separation technologies are discussed and illustrated with several examples. Úplný záznam
	Rozlišení kmenů rodu Candida pomocí kapilární HPLC Vykydalová, Marie ; Moravcová, Dana ; Horká, Marie ; Růžička, F. ; Kahle, Vladislav Cílem této práce bylo rozlišení kvasinkových kmenů porovnáním jejich chromatografických profilů lyzátů. Úplný záznam
	Dynamic modification of microorganisms by pyrene-butanoate for fluorometric detection in capillary electrokinetic techniques Horká, Marie ; Růžička, F. ; Šlais, Karel Pyrenebutanoate and non-ionogenic tenside on the basis of pyrenebutanoate as the fluorescent compounds were used as buffer additives in capillary zone electrophoresis or capillary isoelectric focusing, respectively, for a dynamic modification of the microbial samples. Using the deuterium lamp UV excitation for the on column fluorometric detection, the minimum detectable amount of the microorganisms in order of ones to tens of cells sampled on the separation capillary was achieved. Úplný záznam

Viz též: podobná jména autorů
8	HORKÁ, Michaela
1	Horka, Marian
3	Horká, Markéta
2	Horká, Marta
3	Horká, Martina
8	Horká, Michaela
2	Horká, Monika

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