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Synthesis and structual assignment of novel 5´-epimeric 3´deoxy-3´,4´-didehydronucleoside-5´-C-phosphonates
Petrová, Magdalena ; Buděšínský, Miloš ; Klepetářová, Blanka ; Rosenberg, Ivan
Epimeric 5´-(RS) dialkyl 3-deoxy-3´,4´-didehydro-5´-C-phophonates were prepared by nucleophilic addition of various dialkyl phosphites to 3´-deoxy-3´,4´-didehydronucleoside-5´-aldehydes. Successful separation,crytstallisation and X-ray analysis of a pair of epimeric 5´-C-phosphonates, followed by correlation with a series of NMR parameters, led to efficacious configuration assignment of individual epimers in the mixtures.
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Modeling of nucleolar self-assembly
Blažíková, Michaela ; Heřman, P. ; Malínský, Jan
Mammalian cell nucleoli disassemble at the beginning of mitosis and reassemble again during telophase and the early G1. Dynamics of this process was studied using a model based on entropy driven self-assembly of pre-ribosomal particles generated in a single biosynthetic source. Monte Carlo simulations revealed that such model can explain formation of a large aggregate, a nucleolus, in the vicinity of the source. We examined influence of nucleoplasm properties on dynamics of the aggregate formation.
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