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Analýza sekundárních metabolitů streptomycet Ramanovou spektroskopií in vivo
Mikulová, Adéla ; Petříčková, K. ; Bobek, J. ; Chroňáková, Alica ; Samek, Ota ; Pilát, Zdeněk
Práce se zabývá studiem Ramanových spekter sekundárních metabolitů bakterií rodu Streptomyces, zejména polyenních antifungálních látek. Purifikované izoláty a komerční standardy těchto sloučenin byly využity k pořízení referenčních spekter, ze kterých byly odvozeny spektrální markery charakterizující konkrétní detaily chemické struktury. Verifikace pozorovaných markerů byla provedena pomocí predikcí Ramanových spekter in silico. Následně byly tyto poznatky využity ke spektroskopické charakterizaci složení sekundárních metabolitů in vivo, přímo v bakteriích rostoucích na pevném médiu. Vypracované metodiky pomohou optimalizovat podmínky pro biotechnologickou produkci antibiotik.
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Conductive open-cell silicone foam for modulatable damping and impact sensing applications
Preuer, R. ; Šleichrt, Jan ; Kytýř, Daniel ; Graz, I.
Nature has long served as a source of inspiration for the development of new materials, with foam-like structures in fruits such as oranges and pamelos serving as examples of efficient energy dissipation. In this study, we present the synthesis and characterization of a conductive silicone foam for potential impact sensing applications. By blending Sylgard 184 and Carbon Black, we create a highly porous structure capable of dissipating energy and modulating its resistance. To investigate the properties of the foam, we utilized both micro-computer tomography (μCT) and scanning electron microscopy (SEM) imaging techniques. The μCT imaging revealed the intricate pore network of the foam, reminiscent of the complex structure found in natural sponges. SEM imaging allowed for observation of the uniform distribution of Carbon Black particles within the foam, enabling the conductive properties of the foam. The foam’s mechanical behavior was characterized by a compression test under μCT imaging to measure the deformation behavior and changes in the foam’s resistance. Additionally, a ball drop test was conducted to investigate the foam’s damping behavior while simultaneously measuring the impact location by the local change in resistance. Remarkably, our results demonstrate the exceptional damping capabilities of the conductive silicone foam, with the damping ratio modulated by adjusting the degree of compression-induced deformation. This is attributed to the collapse of the foam’s porous structure, resulting in a significant increase in the foam’s contact area. Overall, our study provides valuable insights into the behavior of conductive silicone foams and their potential as an impact sensing material. The use of both CT and SEM imaging techniques allows for a comprehensive understanding of the foam’s properties, which can be optimized for a variety of applications. The foam’s ability to modulate its damping properties by adjusting the degree of deformation provides a promising avenue for future research in the field of materials science and engineering.
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Effect of the long-term storage methods on the stability of cartilage biomechanical parameters
Žaloudková, Blanka ; Sekorová, Š. ; Kopecká, B. ; Kytýř, Daniel
Long-term stability of the tissue product in terms of mechanical parameters is a key factor for its expiration date. For the investigation of storage effects on the cartilage tissues the experimental mechanical loading test combined with XCT scanning for the irregular shape inspection was performed. The samples were preserved according to three different protocols using the deep-freezing and two types of saline solution preservation. The stability of the biomechanical parameters was tested within annual intervals. All samples were subjected to uni-axial compression loading using the in-house developed compact table top loading device in displacement-driven mode. Based on the measurements, the results are represented in the form of stress-strain curves and quantified as elastic modulus and ultimate compression stress. It can be concluded that no significant difference was found in neither the mechanical properties of the samples nor in the effects of each preservational method.
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