Šalplachta, Jiří - Výsledky hledání - Digitální repozitář

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	Analysis of saccharides in grapes and wine using HPLC with various stationary phases Flodrová, Dana ; Čmelík, Richard ; Šalplachta, Jiří ; Bobálová, Janette The quality of wine is influenced by its saccharides content, especially glucose and fructose. Monitoring of wine oligosaccharides is of great interest, because of their physiological, medical and food importance. The aim of study was to develop method for fast analysis of saccharide composition of wine, grape juice and grapes. Úplný záznam
	IEF and HPLC-based methods for efficient bacterial characterization Kubesová, Anna ; Šalplachta, Jiří ; Moravcová, Dana ; Horká, Marie Pectobacterium and Dickeya species are responsible for blackleg and tuber soft rot diseases on crop and ornamental plants. Recently, a more virulent and aggressive Dickeya type of E. chrysanthemi was described and it becomes serious problem for potato production in Europe. In this study, we suggest procedures using two commonly available laboratory techniques, gel isoelectric focusing and liquid chromatography, for differentiation and characterization of Pectobacterium and Dickeya species. We have confirmed that fingerprinting approach can be used for bacterial differentiation, which can be further used in rapid diagnosis of plant disease. Úplný záznam
	Separation of clinically significant microorganisms from CSF by CIEF with UV detection and MALDI-TOF MS Horká, Marie ; Šalplachta, Jiří ; Kubesová, Anna ; Růžička, F. ; Šlais, Karel The identification of most of the clinically significant species by the conventional laboratory methods is still time-consuming and often insufficient for ensuring early targeted therapy. This is especially important in the case of the nosocomial infections where the number of microorganisms in the cerebrospinal fluid is often ranging from units up to hundreds of cells per milliliter during bacterial infections of the central nervous system. The electrophoretic techniques with UV-detection reach the detection limit of intact cells approximately from 107 cells mL-1. Úplný záznam
	Filtration microcartridge and capillary isoelectric focusing for the analysis low number of microorganisms in real samples Kubesová, Anna ; Horká, Marie ; Šalplachta, Jiří ; Horký, J. At present, detection and identification of microorganisms in real biological samples needs very sensitive methods for correct diagnosis and therapy in medical practice, biotechnology, agriculture, food safety and quality etc. Accordingly, it is essential to develop new cheap techniques for pathogen identification, particularly for pathogen in complex biological samples. Pre-concentration, separation, and sensitive detection of whole cells in one step are great potential and advantage for detection of pathogens in low concentration. Combination of filtration microcartridge and capillary isoelectric focusing was developed for pre-concentration and pre-separation of microorganisms from real suspensions and the possibility its application to real samples was verified. Úplný záznam
	Differences in fingerprints of biofilm-positive and biofilm-negative Candida strains exploitable for clinical practice Vykydalová, Marie ; Horká, Marie ; Šalplachta, Jiří ; Růžička, E. ; Kahle, Vladislav In this study, we have presented differences in fingerprints of Candida parapsilosis and Candida metapsilosis as well as their biofilm-positive and biofilm-negative forms. Fingerprints of yeasts were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis, capillary isoelectric focusing, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Samples for analyses were prepared by treatment of yeasts by boiling water or ethanol. Fingerprints of tested yeasts cells have differed in all cases. This knowledge can be used for obtaining rapid and simple method which distinguishes above mentioned yeasts in standard biochemical laboratories. Úplný záznam
	MALDI-TOF MS of Dickeya and Pectobacterium species - Characterization of plant pathogenic bacteria Šalplachta, Jiří ; Kubesová, Anna ; Horký, J. ; Horká, Marie MALDI-TOF MS was applied to reliable characterization of Dickeya and Pectobacterium species in this study. Our results confirm suitability of suggested analytical approach for efficient bacterial differentiation of species based on species specific mass fingerprints. MALDI-TOF MS of intact bacterial cells require minimum sample handling and is less time-consuming compared to conventional methods. Úplný záznam
	Proteomic analysis of cereal proteins Šalplachta, Jiří Úplný záznam
	Nový přístup v proteomice glutenu pomocí chymotrypsinu a hmotnostního spektrometru MALDI hybrid multistage Šalplachta, Jiří ; Chmelík, Josef ; Allmaier, G. Proteomická identifikace glutenových proteinů v potravinách Úplný záznam

Viz též: podobná jména autorů
1	Šalplachta, J.
6	Šalplachta, Jakub

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