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The influence of some factors on renal impairment in AA amyloidosis
Potyšová, Zuzana ; Ryšavá, Romana (advisor) ; Monhart, Václav (referee) ; Horák, Pavel (referee)
Introduction: Available data suggest an association between presence of secondary (AA) amyloidosis and MCP-1 (monocyte chemoatracttant protein-1) and MIP-1alpha (macrophage inflammatory protein-1 alpha) genes polymorphisms. Some studies have also shown an impact of polymorphisms in exon 3 of SAA 1 (serum amyloid A 1) gene on the incidence of AA amyloidosis in different populations. Methods: The incidence of single genotypes MCP-1, MIP-1alpha and SAA 1 genes was investigated. Serum levels of SAA, MCP-1 and MIP-1alpha were measured and potential relation between serum levels and genotypes were analyzed. All examinations were performed in patients with AA amyloidosis (43), rheumatoid arthritis (RA) without amyloidosis and healthy control group (100). Results: Significantly more frequent occurrence of 1.1/1.1 genotype in SAA 1 was recorded in AA amyloidosis group compared to RA group as well as in control group (p<0,001). No statistically significant differences in distribution of another genotypes were found. Distribution of neither 1.1/1.1 genotype nor another ones did not vary among RA group and control group. No significant difference in distribution of another examined genotypes was recorded among all three groups. Serum concentrations of SAA were statistically significantly higher in AA amyloidosis group...
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Protein in the diet of athletes
Zemanová, Kristýna ; Hučko, Boris (advisor) ; Kodeš, Alois (referee)
Protein plays an important role in an athlete's regeneration, recovery and muscle growth. Unlike fat and carbohydrates, proteins are irreplaceable in our diet. The muscle tissue is composed primarily of proteins, so most athletes believe that higher protein intake will result in faster muscle growth. However, an excessive amount of protein is linked to fatigue, higher strain on kidneys and liver, loss of large amounts of water and potassium ions. Furthermore, high level of protein intake causes excretion of certain vitamins and minerals (e. g. calcium, zinc, B vitamins).
The human body is unable to effectively process more then 2 to 2.5 g of protein per kilogram of body weight. Recommended daily protein dietary allowance is 0.8 to 1.2 g/kg for a sedentary person, 1.2 to 2 g/kg for athletes, approximately 1.2 to 1.4 g/kg for endurance athletes, and for heavy resistance training athletes increased protein intake of 1.6 to 1.7 g/kg is recommended.
Meat and dairy products contain complete proteins that have an ideal ratio of essential amino acids well suited for human dietary needs. In contrast, proteins derived from plant food contain only incomplete proteins. One must combine multiple plant sources to achieve sufficient amounts of all essential amino acids.
There is no need for protein supplements as long as we eat a well-balanced diet. Most athletes, however, use whey proteins to boost regeneration and muscle growth. It is particularly valued for its easy digestibility. Therefore, it is recommended to be taken right after training (at doses of 0.3 g/kg body weight) when the digestive tract is not capable of processing complex food. Increased levels of amino acids in blood soon after consumption are required for effective muscle protein synthesis.
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Antioxidative effects of the analogues of nucleic acid components.
Procházková, Eliška ; Mertlíková Kaiserová, Helena (advisor) ; Zusková, Iva (referee)
Reactive oxygen species (ROS) naturally occur in the organisms where they play e.g. signaling and antimicrobial roles. The imbalance between ROS production and removal results in the oxidative stress. This condition is largely considered as unfavourable leading to the oxidative damage of the biomolecules (lipids, proteins, nucleic acids). The oxidative stress accompanies a number of civilization diseases and may also arise as a consequence of pharmacotherapy (side effects of some drugs). Modulation of the oxidative stress with the use of antioxidants remains a subject of intense research. The analogs of nucleic acid components represent a structurally diverse class of compounds with important pharmacological potential. While their antiviral and cytostatic activities have been described in detail, information on their potential antioxidant activity is missing. The aim of this study was to identify the antioxidant activity in a series of 126 analogs of nucleotides, nucleosides and nucleobases and to describe structural motives responsible for this activity. Several methodological approaches have been used to reach the goal: 1) determination of the ability of the compounds to scavenge ABTS radical in vitro (TEAC), 2) the influence of the compounds on lipid peroxidation and 3) their ability to...
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Problem of the undernourishment and the food water resources
Polzová, Tereza ; Kuna, Zbyněk (advisor) ; Petr, Petr (referee)
The main focus of the final thesis are the problems related to the problem of undernourishment in affected
regions of the world, sea fishing and aquaculture.
In the beginning, the definition of the food problem in the world is introduced. The potential contribution of proteins from the sea production to human dietary is emphasized. Further, the exponential population growth is mentioned, influencing the future development and the food problem itself.
The second part of thesis is dedicated to fisheries and aquaculture. The importance and benefits of sea water resources as well as aquaculture is mentioned from the nutritional and livelihood point of view. Sea management and international regulations are playing
crucial role in the sustainability of the fisheries stocks. These are needed to eliminate the problems of fisheries and aquaculture and the degradation of sea ecosystems due to unsuitable fishing techniques.
At the end of the thesis a summary of all important information is listed. Followed
by possible solutions of these global problems.
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Modelling of the interaction of proteins and peptides with metal ions
Gutten, Ondrej ; Konvalinka, Jan (advisor) ; Obšil, Tomáš (referee)
Modelling of interactions of proteins and peptides with metal ions Ondrej Gutten - Diploma thesis Keywords: Metalloproteins, metal ion selectivity, in silico prediction Abstract: An approach for in silico prediction and estimation of selectivity properties of metal-binding peptides is suggested. An in-depth analysis is performed to disclose the justifiability and limitations of this approach. The study is divided into three parts. First part investigates the soundness of two quantum chemical methods (MP2 and DFT) for their use in the set-up quest. The testing includes comparison with CCSD(T), effect of basis selection, performance of the two methods in geometry optimizations and effect of implicit solvent model. Second part foreshadows the approach of searching for a metal selective peptide by thoroughly investigating the ability of simple representative systems, derived from their metalloprotein templates, to retain the property of interest. Final part describes the initial step of extensive combinatorial approach towards examination of vast number of simple systems that represent metal-binding sites, and which are to be used for prediction of metal-selectivity through exploitation of the described approach and, ultimately, to the de novo design of metalloproteins with desired properties.
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Physiotherapy at high amputations as a complication of diabetes mellitus
HAJNÁ, Štěpánka
A topic of this bachelor thesis is "Physiotherapy at high amputations as a complication of diabetes mellitus." Diabetes mellitus is a chronic, etiopathogenically heterogeneous disease. It arises from the lack of an insulin action at its absolute or relative absence and it is accompanied by a complex metabolic disorder of sugars, fats and proteins. Long-term vascular complications, which can lead to an amputation, gradually develop on the basis of this disease. An introductory part of the current state is focused on the characteristic of diabetes mellitus, its classification, risk factors, pathogenesis, course and its complications. The other part is focused on the problem of amputations and the last part of the current state is focused on used examination methods and physiotherapeutic procedures. A goal of the research part was to evaluate a usage of physiotherapeutic methods at patients with the high amputation as a complication of diabetes mellitus and a possibility to integrate these patients into a life in the highest possible quality. I used, for the practical part of the bachelor thesis, methods of the qualitative research, the case study and an analysis of personal documents. The tested sample consisted of two patients with the high amputation, which was indicated because of complications of diabetes mellitus. The patients were monitored during their hospitalization in the rehabilitation ward or in the successive care ward of the hospital in České Budějovice. The results have been worked up in the form of case reports. The health status of all patients was at least partially improved and it came about their better integration into the society. It is therefore possible to say that rehabilitation goals were met. The bachelor thesis can be used in the actual work of physiotherapists or as a basis for their further scientific research. Then it can be used by patients themselves or by their close people in dealing with this handicap.
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Impact of temperature and drought on gliadins and glutenins contents in four varieties of wheat
Tomasz, Teresa ; Vítová, Eva (referee) ; Hrstka, Miroslav (advisor)
This diploma thesis deals with an influence of high temperature and water shortage on the protein content of gliadin and glutenin fractions in four varieties of winter wheat: Bohemia, Tobak, Pannonia and var. Syria with designation S46 (IG142780). The crop was grown at 26, 29, 32, 35, 38 and 41 °C during anthesis under control irrigation treatment (with soil moisture higher than 70 %) or under drought stress (with soil moisture lower than 30 %). To separate gliadins, the A-PAGE method was used, and glutenins were separated by SDS-PAGE method. Proteins were quantified by computer densitometry. Significant influence of genotype on the gluten proteins was found. Variety Pannonia has high content of -, 5-gliadins, LMW and HMW glutenins, but low content of other gliadin fractions. It was the opposite in the other varieties. Due to temperature, as well as drought, there was an increase in the content of all gluten fractions, especially of HMW glutenins, 1,2-gliadins and total gliadins. The largest increase in the gluten fractions due to drought was observed in Syria variety. In other varieties simultaneous exposure to drought and heat caused decrease in gliadin content, but increase in glutenin content. Drought at high temperatures reduced gliadin-to-glutenin ratio, mostly in Bohemia variety. This ratio has increased due to the temperature, especially in Tobak variety. For Syria variety, no effect of stress conditions was found on gliadin-to-glutenin ratio.
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Vliv výživy na poruchy metabolismu krav
Matějíček, Martin
The aim of PhD thesis was to check effects of balanced mixed feeding rations of dairy cows at the different stages of reproduction cycle, to ensure minimal occurrence of metabolic diseases, and improve the health of animals. Selected indicators for the metabolic profile of blood plasma in 10 cows in each established group (calving preparation, early lactation and peak of lactation) were measured. In terms of protein metabolism we found in two cases significant differences in plasma total protein concentration of cows in groups calving preparation and early lactation. The concentration of bilirubin in plasma increased in the group of early cows. But there were no found significant differences. On the contrary, in the case of AST, significant differences between cows in group calving preparation and early lactation were detected. Parameters of lipids metabolism did not significantly change as well as parameters of mineral metabolism. The blood plasma calcium concentrations show a significant difference in the groups calving preparation and early lactation. The highest values of calcium achieved cows in the group of calving preparation.
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Catalytic and Electronic Properties of Redox-Active Metalloenzymes and Transition-Metal Complexes: Insights from the Computational Chemistry.
Srnec, Martin ; Rulíšek, Lubomír (advisor) ; Himo, Fahmi (referee) ; Papai, Imre (referee)
Charles University in Prague Faculty of Science Department of Modeling of Chemical Properties of Bio- and Nanostructures Catalytic and Electronic Properties of Redox-Active Metalloenzymes and Transition- Metal Complexes: Insights from the Computational Chemistry Dissertation Thesis Abstract RNDr. Martin Srnec Supervisor: Mgr. Lubomír Rulíšek, CSc. Institute of Organic Chemistry and Biochemistry AS CR Center for Biomolecules and Complex Molecular Systems Praha 2010 Introduction Metals and their ions play a key role in maintaining life. They frequently promote protein folding, the stabilization of protein scaffolds, enzymatic activity, energy conversion, intra- and intercellular signals etc. Several decades ago, metalloproteins were considered as a rather small group of proteins. Nowadays, one-third of enzymes are estimated to contain one or more metal ions, whose presence is crucial for their enzymatic functionality. Metalloenzymes participate most often in the catalysis of difficult chemical reactions (e.g. the hydroxylation of methane, decomposition of H2 into protons and electrons, N2 and O2 bond cleavage and many other examples), in oxidation-reduction and electron-transfer reactions, and in the catalysis of spin-forbidden reactions, where relativistic effects (i.e. spin-orbit coupling) are necessary....
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Bezlepková dieta
Malaníková, Šárka
Gluten-free diet is very important in the celiac disease treatment. If we eliminate the food containing the gluten, the cause of the disease could be removed. Gluten is a part of the prolamine fraction of proteins which can be found in the in the endosperm of seeds of some cereals such as wheat, rye, barley and oats. Gluten substitution in the food meant for gluten free diet is a substantial technological problem, especially when making fine tasty gluten free bakery products. Therefore it is important to search for new ingredients for making gluten free food. Gluten free diet influences people's life greatly. Managing of the gluten free diet demands a big effort and motivation especially for the fact that it is a lifelong change. It is thus necessary to search, analyse and deal with the new possibilities for life quality improvement of the patients with the celiac disease.
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