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The Youth of selected infectious alimentary disease preventiv in the region Milevsko
VINKLEROVÁ, Lucie
Foodborne diseases form a large group of infectious diseases. These substantial diseases are occurring more in developing countries but they do not avoid developed countries. The originators of the disease are bacteria and their toxins, viruses, parasites and prions, whose source is an infected person or animal. The disease is passed on by contaminated soil, contaminated water and food, sometimes contaminated items. Greatest importance in the prevention of foodborne disease are nonspecific measures, protection of drinking water, safe food production, waste disposal, functional sewer system, rodenticides and insecticides measures and of course the principles of personal hygiene, certain standards of hygiene and health education of the population. Intestinal infections are preventable. The consumer can effectively protect by following the fundamental principles established by the World Health Organization. The aim of this study was to explore knowledge of foodborne disease prevention measures and compliance with these principles by adolescents in the Milevsko region. The theoretical part of the study summarizes the basic information about intestinal diseases and describes their effect on health from a variety of perspectives. The preventive measures which everyone should be aware of and apply them in everyday life to protect health are described in great detail. The paper summarizes the information about the process of spreading foodborne diseases and epidemiological measures focused on supply, routes of transmission and susceptible individuals. The theoretical part includes basic information about specific intestinal infections which are most significant for the population in the Czech Republic. Briefly described are treatment of these diseases and the most important legislative measures. In the research part quantitative research was applied by questionnaire. The research groups were students of higher secondary vocational schools and vocational schools in the region Milevsko. Three hypotheses were formulated. Statistical hypothesis testing was performed using the t test. The values of the achieved significance level are compared with a predetermined level of significance. The first hypothesis assumed that knowledge about prevention of intestinal diseases is higher among girls than boys. This statement has not been confirmed. Knowledge of the principles of prevention does not differ according to the criteria of gender. Two further hypotheses assumed that the observance of hygienic measures in the prevention of foodborne disease is higher among girls than among boys and higher for high-school graduates than apprentices. These hypotheses are valid. Precautionary principles are indeed respected more by girls than boys and high-school graduates than apprentices. The carried out research shows that adolescents in the region Milevsko have a good knowledge of foodborne infections and do known also essential preventive measures. However, knowledge of these rules does not mean they are applied in practice. The results showed that some rules, such as washing fruits and vegetables before eating, adequate boiling or roasting of food, especially hand hygiene before eating food or after contact with an animal are observed by a smaller percentage of respondents than that reported knowledge of these principles. A positive finding is that respondents comply with certain rules, without being aware of them, such as the principle of proper preservation of precooked food. Foodborne diseases are diseases which can be prevented in compliance with precautionary principles. Especially in the Czech Republic, where nonspecific principles are at a high level. It is up to each user/consumer how consistent these principles will be. Respecting preventive measures is a prerequisite for reducing the incidence of foodborne disease in the population.


Monitoring of natural occurence of mycoparasitic and antagonistic fungi in soil in the region of South Bohemia
BÍLKOVÁ, Jana
The thesis is focused on monitoring of natural occurrence of mycoparasitic fungi in arable soils. Study was aimed to isolate those fungi from selected plots with conventional and organic farming systems of the region of South Bohemia. Four isolated method were used for isolation of mycoparasitic fungi from soil.Among all, species of genus of Trichoderma, Trichoderma virens, Clonostachys rosea f. catenulata and Lecanicillium muscarium were the most frequently present and isolated species.The radial growth of strains and their production characteristic was evaluated. The efficacy of Trichoderma strains against phytopathogenic fungi (S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea a Fusarium solani) was evaluated.


Importance of denitrifying microorganisms in terrestrial ecosystems - focus on soil micromycetes
ROTTNER, Lukáš
This thesis summarizes the aspects of ecological importance regarding denitrification of soil and discusses its origin in fungal denitrifiers. Also it describes the biochemical mechanism of fungal denitrification together with the options for denitrification measurement and micromycelial denitrifiers cultivation.

The application of Brdička reaction for determination of phytochelatins in real samples of barley and thermophilic cyanobacterium grown in the presence of cadmium ions
Čížková, Petra ; Fedjuk, K. ; Šestáková, Ivana
Heavy metals represent important group of environmental contaminants. Ions of heavy metals (Cd, Pd, Cu, Hg, Zn and Ni) are released into soil solutions as a consequence of soils acidification, among others. Their presence has imminent influence on physiological procedures in plants, which have developed different mechanisms of adaptation on such conditions. One of mechanisms for plants tolerance towards heavy metal ions is their inactivation by binding on polypeptides or small proteins with high cysteine content, named phytochelatins (PC) or metallothioneins. Their function has been studied in particular at man and animals.

Bioavailability of PAH and its effects on biodegradation during composting
Cajthaml, Tomáš ; Hubálek, Tomáš ; Šašek, Václav
The publication deals with application of composting process on remediation of soils contaminated with PAHs. Bioavailability of PAHs was tested with sequential SFE and the data were compared with the results of remediation. SFE is suitable to predict results of bioremediation.

The use of transgenic plants for the remediation of soils contaminated with cadmium
Čásová, K. ; Pavlíková, D. ; Macek, Tomáš ; Najmanová, J.
Study investigates if genetically modified tobaccos have higher ability to accumulate and tolerate cadmium, and to compare their phytoextraction characteristics with other plant species.