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Expression of cholinergic gene locus in a mouse model of Alzheimer's disease
Zimčík, Pavel
(anglický jazyk) The most common senile dementia, Alzheimer disease (AD), is characterized by a decline of memory and high cognitive functions. Typical post-mortem brain lesions are extracellular amyloid deposits, intracellular neurofibrilary tangles and ruined cholinergic and other neurotransmitters systems. Connection between damaged central cholinergic system and beta-amyloid accumulation remains obscure. We examined parietal cortex of young adult (7- month-old) female APPswe/PS1dE9 double transgenic mice which develope beta-amyloid fragments at high rate. Cholinergic synapses of these mice demonstrate functional presynaptic (stimulated acetylcholine release) as well as postsynaptic (muscarinic receptor-induced Gprotein activation) deficits and reduction of cholinergic markers. The mRNA levels of choline acetyltransferase, vesicular acetylcholine transporter and M1 to M4 subtypes of muscarinic receptors were determined in transgenic and littermate controls using qPCR. Obtained experimental data does not show any changes in measured mRNA levels. These observations indicate that reduction of cholinergic synaptic markers and function is due to posttranscriptional events.
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Statistical analysis of social services level for the elderly in capital city Prague
Brezováková, Petra ; Prášilová, Marie (advisor) ; Irena, Irena (referee)
The diploma thesis focuses on analyzing the development and level of social services for the elderly in Prague. Examined facilities of social services for the elderly were daycare centers and day centers for the elderly, retirement homes and special regime homes for the elderly with Alzheimer's disease. For the processing and analysis of selected indicators, the number of social services, distribution of homes by type, number of capacities in social institutions and social services funding through subsidies and grants for the monitored period 2007 - 2015, elemental characteristics of time series and future economic developments for the following years forecasted by suitable function are used. The thesis includes its own survey among providers of special homes for the elderly with Alzheimer's disease. Based on the analysis there are formulated recommendations that could contribute to the further development of social services and maintain quality of care for seniors in Prague.
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