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Determination of macroelements, organic acids and other parameters in ketchups
Popelová, Nikola ; Divišová, Radka (referee) ; Diviš, Pavel (advisor)
The aim of this master’s thesis is perform basic analyses of samples ketchups. The theoretical part describes the basic characteristics of tomato, tomatoes processing and production of puree and ketchup. Then there are described analytical methods which were used for analysing the selected parameters in ketchups. The experimental part deals with the determination of pH, total solids, organic acids, saccharides, macroelements and lycopene in ketchups. Citrate and acetate were analysed by IC. The amount of acetate in ketchups was about 5 times greater than the amount of citrate. Glucose, fructose and sucrose were analysed by HPLC. Glucose and fructose were determined in all samples but sucrose not. The ICP-OES method was used to determine four elements. Magnesium was the least represented element and sodium was the most represented element in the analysis. The amount of sodium was recalculated on the amount of salt. Lycopene was analysed by UV-VIS spectrometry. Its amount in ketchups is influenced by the variety of used tomatoes and plenty of other factors.
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Determination of content substances for selected species and varieties of roses.
Slavíková, Eva ; Sus, Josef (advisor) ; Súkeníková, Tereza (referee)
This bachelor's work is focused on comparing the most significant content substance, vitamin C, in fruits of chosen rose kinds species and varieties. Herbal products are often seen by people as products of "second choice", when failure of allopathic treatment occurs, which is focused especially on suppressing symptoms of illness, but it ignores health of the human body as whole. That's why are herbal products often alternative for therapy of chronic diseases. The base of this work is extensive literary research and results of laboratory measurings. Literary part of this work includes description of botanical classification of rose plants. There is stated basic characteristic of individual rose species and their morphological features. In following chapters is composed overview of effective substances in rose fruits and their effect on human health. In practical part of this work are included results of laboratory tests for determining ascorbic acid content for chosen samples of rose species and it also includes results of weighing and measuring of fruit samples of chosen rose species. There are also stated chosen rose species and shortly their origin and description, in the beginning of practical part. For practical part were chosen samples of species. Results of practical part are compiled into statistic tables and graphs and it confirms high content of ascorbic acid in rose fruits. This content is in range of cca 300 - 900 mg.100 g-1. Due to growing interest in alternative treatment options and "clean" natural product, it can be assumed, that many plants of Rosaceae family will keep its place in treatment practice. Those plants will be used not only for treatment of acute and chronic diseases, but also for cosmetic products production, thanks to its large effects and process options.
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Formation of yield and dry matter with genetic resources of opium poppy (Papaver somniferum L.)
Slowiaczek, Lukáš ; Hnilička, František (advisor) ; Jan, Jan (referee)
Diploma thesis with the topic: Formation of yield and dry matter with genetic resources of opium poppy (Papaver somniferum L.) was worked out at the Department of botanics and plant physiology, Faculty of agrobiology, food and natural resources ČZU in Prague. The purpose of this thesis was to determine genotype and ontogenetic differences in amount of yield, production of dry mass, and value of growth-analytic characteristics in certain species of opium poppy. Diploma thesis was based on the fact that most of the studies about opium poppy (Papaver somniferum L.) are focused on the content of alkaloids and other substances present in poppy seeds. Due to this facts, it is necessary to describe the genotypes of poppy plants and their genetic resources from the physiological point of view.
Following species of opium poppy were chosen for the experiment: Akvarel, Albín, Buddha, Florian, Korneuburger Weisser, Lazur, Major, Marianne, Opál, Postomi, Sokol, Tatranský. These species were chosen according to their content of alkaloids, especially morfine very low content, low content, medium content and high content of morfine. All of the tested genotypes are tested as object of study of genetic poppy resources. Seeds were supplied by GB Oseva Pro s.r.o., o.z. VÚO Opava and company Český mák.
Field experiments were established at the research laboratory FAPPZ ČZU in Prague, in Červený Újezd. This location is in Prague-West division, in area of Middle Bohemia. The altitude of this location is 401 metres above the sea level. The size of the field for testing was 2 m2. The experiment was based in 4 repetitions by the method of Latin square. Winter wheat was used as the preceding crop. Opium poppy was planted according to agrotechnical principles of planting.
Content of dry mass in individual plant organs was measured in certain ontogenetic stages of opium poppy (35, 45, 49, 52, 54, 62, 81 BBCH). Size of leaf area was assessed by the image analysis WinDias. Yield of seeds was assessed as well. Growth-analytic characteristics were calculated from the values of wieght of dry mass and size of leaf area.
The results confirmed that the size of plants is influenced by its ontogeny and also by its species. The results also confirm, that the height of plants is not influenced by the content of morfine. Connection between the colour of seeds and height of plant was not confirmed either. Increase in weight of above-ground biomass depending on ontogenetic stage and influence of plant species was confirmed. There are differences in production of yield in species of poppy with different contents of morfine because the average weight of yield in species with very low content of morfine was 112,36 g, in species with low content of morfine 110,41 g, in species with middle content of morfine 12167 g and in species with high content of morfine 123,16 g. It was approved that ontogeny of plants influences the leaf area index (LAI) as it increases until blooming and then it decreases as a result of senescence and falling off the old leaves. Differences in leaf area index were detected among the species of opium poppy, depending on the content of morfine. It was detected that crop growth rate (CGR), relative crop growth rate (Rw) and relative growth rate of assimilation area (RA) are influenced by the ontogeny of plants. It was detected that species with high content of morfine in poppy seeds (Buddha, Postomi, Lazur) and species with white seeds Sokol and Albín have lower yield than species with middle content of morfine.
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Determination of content substances for selected species and varieties of roses.
Slavíková, Eva ; Sus, Josef (advisor) ; Súkeníková, Tereza (referee)
This bachelor's work is focused on comparing the most significant content substance, vitamin C, in fruits of chosen rose kinds species and varieties. Herbal products are often seen by people as products of "second choice", when failure of allopathic treatment occurs, which is focused especially on suppressing symptoms of illness, but it ignores health of the human body as whole. That's why are herbal products often alternative for therapy of chronic diseases. The base of this work is extensive literary research and results of laboratory measurings. Literary part of this work includes description of botanical classification of rose plants. There is stated basic characteristic of individual rose species and their morphological features. In following chapters is composed overview of effective substances in rose fruits and their effect on human health. In practical part of this work are included results of laboratory tests for determining ascorbic acid content for chosen samples of rose species and it also includes results of weighing and measuring of fruit samples of chosen rose species. There are also stated chosen rose species and shortly their origin and description, in the beginning of practical part. For practical part were chosen samples of species. Results of practical part are compiled into statistic tables and graphs and it confirms high content of ascorbic acid in rose fruits. This content is in range of cca 300 - 900 mg.100 g-1. Due to growing interest in alternative treatment options and "clean" natural product, it can be assumed, that many plants of Rosaceae family will keep its place in treatment practice. Those plants will be used not only for treatment of acute and chronic diseases, but also for cosmetic products production, thanks to its large effects and process options.
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Evaluation of selected qualitative parameters of apples produced under different growing methods
Dresslerová, Ivona ; Kouřimská, Lenka (advisor) ; Jan, Jan (referee)
The most spread system of agricultural production is conventional system, which reaches the best profits by its excessive inputs into production such as fertilization, chemical spraying, heavy engineering. However the importance of ecological (BIO) and integrated (I.P.) production has been increasing among the consumers recently. Mainly because of environmental sustainability and protection of their own health. The aim of this study is to compare qualitative characteristics of 10 different apple cultivars (Melodie, Šampion, Gloster, Idared, Angold, Topaz, Goldstar, Ontario, Florina, and Rubín) with reference to the type of production system. The dry matter content, amount, concentration of carbohydrates, ascorbic acid, sensory evaluation and overall profile of volatile compounds were analysed in apples coming from both ecological and integrated agriculture.
The dry matter content of all apple cultivars was measured by using two different methods. At the same time drying in an infra-red scale and refract metrical measuring by a portable refractometer were carried out. Concentrations of different carbohydrates (sucrose, glucose and fructose) were analysed by HPLC method with RI detection. The content of ascorbic acid (AA) was determined by HPLC with UV/VIS detection. During the sensory evaluations a protocol for a trained sensory panel was created including profile, pair and pair preference test. With an aim of detection of volatile aromatic compounds´ profile there was a sorption prepared on SPME extraction with following analysis on GC-GC-MS.
In the concentration of C vitamin was the cultivar found statistically significant (p = 0,0004) and not in the supposed type of production. The final concentration of ascorbic acid was 13-130 mg/kg in BIO apples and 10-103 mg/kg in apples from integrated production. Overall 7 BIO cultivars had higher content of AA in comparison to I.P. The highest concentration of AA was in cultivar Ontario. In total 7 BIO cultivars from 10 had bigger content of AA in addition to integrated samples. The most present carbohydrate in apples is fructose in which was found a statistically significant difference between values of BIO and I.P. (p = 0,0272). The highest figure of fructose and also of sum of sugars was Angold BIO (422,3 g/kg). The highest presence of dry matter was found in cultivar Florina BIO (18,62 %). Refract metrical matter was in the range from 11 to 17 Brix.
There was a statistically significant importance proved between the figures of BIO and I.P between both dry matters. At the same time it was proved that there is present a dependence between them based on correlation coefficient (r = 0,9622). It was assumed that the overall sum would correlate with refract metrical dry matter, but this was not confirmed. There was also proved a statistical difference (p = 0,0002) between the BIO and I.P apple weights. In all cases of samples the weights of I.P apples was higher. In sensory analysis the cultivar Šampion BIO was evaluated the best from the overall intensity and pleasure of the taste.
Topaz I.P was evaluated as the sample with the most pleasant taste and Florina I.P with the highest taste intensity. By the analysis of pair preference test results it was confirmed that there is a statistically significant difference on the selected level of probability 99%. But preference in any of production systems was not confirmed. During the analysis of overall profile of volatile aromatic substances it was found out that the same cultivar of different production has the same composition, but different relative concentration of the present substances.
This problematics needs further and more detailed analysis, which would prove or disprove influence of the cultivar on all analysed aspects.
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Effect of different width of rows on biomass yield and dry matter content at growing sorghum
KUBEŠ, Pavel
The aim of this thesis was to determine the effect of row spacing on sorghum yields and dry matter. The trial was established on 29 May 2015 on land belonging to the Agricultural Cooperative Milevsko, which is situated in the corn-growing area. The trial compared four varieties of sorghum, namely Aristos, Goliath, KWS Freya and KWS Sole. The varieties were sown in different row spacings, namely 75 cm, 60 cm, 45 cm a 30 cm. The samples taken on three dates were analysed for dry matter content. Each variety showed a gradual increase in the dry matter content. During the harvest on 5 October 2015 the varieties KWS Freya and KWS Sole reached an optimal value of the dry matter when planted in all spacings. All varieties produced the highest average yield of green matter when planted in rows spaced 60 cm apart. The variety Aristos produced the highest average yield of green matter. The KWS Sole variety reached the lowest average yield of green matter when planted in rows 76 cm apart. All varieties with 60 cm row spacings, except for KWS Sole, produced the highest average yield of dry matter. The highest average yield of dry matter was reached by Aristos and the lowest by KWS Sole. The results of the one-year trial did not prove a direct connection of the effect of row spacing on dry matter. The effect of row spacing on green and dry matter yield was proved. The optimum row spacing seems to be 60 cm.
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The posibility of applying corn hybrids with FAO number 300 in less favored areas
SLANEC, Roman
The aim of this thesis was to evaluate and compare production of biomass and dry matter of silage corn hybrids with different time of earliness - Ronaldinio (Z 240/S 250), Agro Vitallo (Z 270/S 280) and Balasco (Z 370/S 380). This experiment was realized on a field near České Budějovice ( 400 m.a.s.l.) and the resuls were compared to results of a pilot plant experiment realised by KWS OSIVA s.r.o. in ZOD Podhradí Choustník ( 562 m.a.s.l.). All startings of phonological phases were studied according to BBCH-scale in the course of vegetation period. Samples were taken before the harvest to test for contents of dry matter in biomass. They were used to determine the optimal harvest period. These parametres were observed during the harvest : plant height, biomass yield, dry matter of biomass, corncobs yield, share of corncobs, dry matter of corncobs, dry matter yield. The resuls showed succesful use of corn hybrids with higher FAO number (Balasco) in areas of less favourable conditions. Corn is one of the plants very dependent on the course of the year.
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Vliv mléčné užitkovosti a ročního období na kvalitu mleziva dojnic českého strakatého skotu ve vybraném podniku
Provazník, Jan
The purpose of diploma thesis was evaluate the effect of milk production an the season on the quality colostrum Czech fleckvieh cattle in a selected breeding. Between monitored parameters were included solids, active acidity, density, imunoglobulins content and refraction. Literary review was processed to the given problem. Viewed samples came from the farm Agrodružstvo Tištín. Samples were taken during the whole year (from 17. 4. 2014 to 18. 1. 2015). In this period has been taken 39 samples of colostrum, from selected cows. These cows were all on the second lactation. The measured values were statistically processed. The results indicate, that cows with high milk production have better quality of colostrum. Seasons affected the quality of colostrum. The cows, who gave birth in the autumn and winter, had colostrum quality. We found, that healthy cows had in colostrum more immunoglobulins and solids, during the assessing the health condition.
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Stravitelnost živin z praktické krmné dávky pro koně
Pressová, Petra
The aim of the study was to determinate digestibility of nutrients from diets for horses. The experiment was conducted on 9 Czech Warmblood horse breed on University school farm at Žabčice. For selected horses we found weight, age and workload. Horses were divided into 3 groups and in three balance periods were have gradually fed them the same amount of feed mixtures and meadow hay. Altogether an attempt fed 3 compound feed (mixtures), in which we analyzed components that were used in the experiment. Feed mixtures consisting from barley, oats, flaxseed meal, bean, dried whey, soybean meal, mineral premix and meadow hay. Diets mutually differed amounts of soybean meal. The experiment was arranged factor - so that no group of horses did not receive the same mixtures of 2x. The results were found to amount spent nutrients and calculated their digestibility. Digestibility of dry matter was 74.07 %+-4,45, crude fat 30.95 %+-23,62, energy 74.57 %+-3,89, nitrogen-free substances 79.79 %+-4,12, crude ash 66.31 %+-14,89, crude protein 80.17 %+-4,59 and crude fiber 69.91 %+-6,64. Digestibility of calcium, we estimated at 75.08 %+-8,35 and phosphorus 51.65 %+-15,93. Due fed mixture resulting difference in digestibility of crude protein at 1.02 % and the group fed mixtures, soybean meal and the group fed mixtures with the highest content of soybean meal (S10). The digestibility coefficients are most affected by the individual characteristics of horses and will need to make further balance for us to objectively determine the digestibility of crude protein in rations.
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Provozní diagnostika bioplynových stanic
Dokulilová, Tereza
Tato diplomová práce stručně popisuje proces anaerobní fermentace, rozdělení bioplynových stanic a vybrané parametry, které byly sledovány v praktické části. V praktické části byly při laboratorním testování anaerobní fermentace v Celorepublikové referenční laboratoři bioplynových transformací na Mendelově univerzitě v Brně sledovány následující parametry: složení a množství vznikajícího bioplynu, konduktivita, oxidačně redukční potenciál, pH, obsah sušiny, celkového organického uhlíku, celkový obsah dusíku v sušině a poměr C:N ve zpracovávaném materiálu. Výsledky analyzovaných parametrů byly vyhodnoceny, interpretovány a na jejich podkladě byly navrženy možnosti jejich použití pro screeningovou diagnostiku provozních stavů bioplynových stanic.
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