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Preparation of silicalite-1 composite membranes
Hrabánek, Pavel ; Bernauer, B. ; Zikánová, Arlette ; Krystl, V. ; Kočiřík, Milan
Silicalite-1 crystal layers were synthesised on stainless steel and ceramic supports. The stainless steel supports manufactured by Trumem Int. (USA) and ceramic supports manufactured by Carborundum Electrite (Czech Republic) were chosen to prepare silicalite-1 composite membranes. In the case of stainless steel silicalite-1 composite membrane, the applied synthesis process has been recently developed by Gora et.al [1]. It was observed that the presence of silicalite-1 seeds influenced the membrane quality. A number of crystallization steps also played an important role. Ceramic silicalite-1 composite membranes were prepared on ceramic supports with the layers of randomly oriented silicalite-1 crystals. The disadvantage of the ceramic supports was their large pore size distribution and roughness of the surface. For this reason, several coating steps using ceramic glaze had to be used to reduce the pore size distribution and non-uniformity of the surface.
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Fenomén tůně: úvod do morfologie, hydrologie a limnologie
Pithart, David ; Pechar, Libor ; Hrbáček, Jaroslav
Small size of pools result in large interaction with the surrounding terrestrial ecosystem. 1.Wind protection caused by banks and vegetation results in stable water column. 2. Shading of vegetation restrict the amount of light, reaching the water surface. 3. Inflows of underground water may influence the water chemistry substantially. 4. Bottom processes influence the whole water volume in larger extent compared to large lakes.
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Rozdílná jaderná lokalizace aberantních a normálních homologů chromosomu 8 v intaktních nebo NaBt diferencovaných buňkách HT-29
Harničarová, Andrea ; Bártová, Eva ; Kozubek, Stanislav
An accumulation of numerous chromosomal structural aberrations is a typical feature of the human colon adenocarcinoma cell line HT-29. In these cells the chromosome 8 territories were visualized using fluorescence in situ hybridisation (FISH), which enabled to distinguish the aberrant chromosome 8 homologue from the normal one. Analyses of nuclear parameters revealed differences in radial positioning between chromosome homologues analysed. In comparison with an normal chromosome 8, the aberrant chromosome 8 territory was positioned closer to the nuclear periphery. Under standard conditions HT-29 cells displayed relatively undifferentiated phenotype but incubation of these cells in the presence of sodium butyrate (5mM) induced biochemical and morphological changes that are typical of well-differentiated phenotype of enterocytes.
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Analýza genové exprese kolorektálního karcinomu detekované cDNA mikročipy pomocí kombinace různých vyhodnocovacích přístupů
Jansová, E. ; Krontorád, P. ; Svoboda, Z. ; Koutná, I. ; Pavlík, T. ; Kozubek, Michal ; Jarošová, M. ; Žaloudík, J. ; Kozubek, Stanislav
We have compared colorectal carcinoma tissues with parallel samples of epithelial tissue and identified, by means of Human 1.7K cDNA microarrays, a set of genes with significantly altered expression in 12 patients. We used a combination of several approaches of microarray data analysis to be sure that the results were reliable. Although we have found differences between the results obtained by various image analysis software packages and using different statistical tools, we have identified the group of 22 genes with significantly altered expression in colorectal carcinoma tissue as compared with epithelial tissue using all evaluation approaches. Hierarchical clustering showed the possibility of dividing the patients into two groups according to the presence of metastases in regional lymph nodes. We have proposed 6 genes as potential markers for the detection of the presence of regional metastases in patients.
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Dynamika pohybu a umlčování transgenních lokusů HP1 v živých buňkách
Ondřej, Vladan ; Kozubek, Stanislav ; Lukášová, Emilie ; Matula, Pa. ; Matula, Pe. ; Kozubek, Michal
Intranuclear localization and mobility of Cy3 labelled transgene loci were studied in living cells. Observations showed occurrence of several transgene copies in each cell nucleus. The majority of copies were localized in the centromeric heterochromatin defined by HPlbeta-GFP fusion protein, the minority of copies in euchromatin. The tracking of loci showed restricted diffusive motion of these in the short-time range. During long-time observation of HPlbeta domains and transgene loci, we have found that in most cases the proximity of these objects decreased within time. The changes of positions of HPl domains were very small but transgene loci displayed directional movement towards the relevant HPl domain. Our data records support the idea that the cell nucleus consists of several separated higher-order compartments. The genes relocate within these compartments, which is finally connected with changes of their expression status.
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Methylace histonu H3K9 v lidských krevních buňkách a v granulocytech pacientů s myeloidní leukemií
Lukášová, Emilie ; Falk, Martin ; Kořistek, Z. ; Kozubek, Stanislav ; Grigoryev, S. ; Kozubek, Michal ; Ondřej, Vladan ; Kroupová, I.
Common heterochromatin antigenic protein markers while present in human blood progenitor CD34+ cells, differentiated lymphocytes and monocytes are absent in neutrophile granulocytes and, to large extent, in eosinophiles. In acute CML and AML, strong methylation of H3K9 and all isoformes of HP1 are detected. In chronic forms of CML, no strong correlations among the level of histone methylation, disease progression and modality of treatment were observed. Reprogramming of leukemia HL60 cells to terminal differentiation by retinoic acid does not eliminate histone H3K9 methylation and the presence of HP1 isoformes from differentiated granulocytes. Our study shows for the firs time that histone H3 methylation may be dramatically changed during normal cell differentiation.
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Ocenění společnosti Česká spořitelna, a.s.
Voglová, Marcela ; Pavlová, Eva (advisor) ; Březnová, Iva (referee)
Práce se zabývá zjištěním hodnoty společnosti Česká spořitelna, a.s. metodou účetní hodnoty, tržního srovnání a metodou výnosovou a porovnává výsledky těchto metod. Popisuje specifika finančních institucí, zpracovává finanční analýzu, strategickou analýzu a finanční plán České spořitelny. Zmiňuje pojem "hodnota" a "rating" a přibližuje rizika v bankovním sektoru, účetní pravidla v popisované společnosti a strategii společnosti.
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