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Vliv chovu na kvalitu tuku kapra obecného (Cyprinus carpio)
ZAJÍC, Tomáš
It is the sad fact, that in the Czech Republic, consumption of fish is very low compared with the European Union average, but, at the same time, it belongs to the countries with the highest incidence of cardiovascular diseases. Sufficient intake of n-3 poly-unsaturated and highly-unsaturated fatty acids (n-3 PUFA and HUFA) is important in preventing and treating cardiovascular disease. Fish is the main source of these compounds in the human diet. This thesis examined factors influencing n-3 PUFA and HUFA levels in fish lipids and ways of increasing the n-3 fatty acid (FA) content in freshwater farmed fish.First, production of common carp (Cyprinus carpio L.) with defined flesh quality using a finishing feeding strategy and prediction of fillet FA by a dilution model were investigated, the effects of purging on lipid content and fillet quality of common carp in Central Europe were examined in fish kept for up to 70 days in clear water without feeding; and changes caused by frying on fat uptake, fatty acid composition and oxidation in the fillet of carp with increased n-3 fatty acid content were examined using four different fats to fry either plain or battered fillet.
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Comparison of biometrical and slaughtering indicators of crossbreeds of common carp with using two different breeds of Amur mirror carp
PRCHAL, Martin
The objective of this thesis was to compare biometrical and slaughtering indicators of common carp crossbreeds using two different breeds of Amur mirror carp in performance testing. We applied top-crossing for the test establishment, using the Hungarian mirror carp (M2) on the maternal position. Males of Hungarian mirror carp (for the production of purebreed), Northern mirror carp (M72) and two breeds of Amur mirror carp (ALP ? bred in Pohořelice, ALV ? bred in Vodňany) were crossed on this breed and hybrids of scaly phenotype between Ropsha (ROP) and Tata carp (TAT) were used as a control group. The performance testing started in ponds (in the total of five localities) in the semi ? intensive way of management by releasing yolk sac fry (K0) and its subsequent rearing to the market size (K3). The performance test was completed in ponds after the third year of testing and the tested groups of fish were evaluated for biometrical and slaughtering indicators. In the overall evaluation of the edible parts of the body (processed body and fillets) by the ANCOVA method was not found a statistical difference among the mirror tested groups of fish. The only exception was the proportion of fillets without skin, which was significantly higher in the hybrid M2 x ALP than in breed M2, therefore I would not recommend this breed (M2) to commercial breeding. When comparing my results with other publications, the hybrid M2 x M72 could be used for commercial breeding together with the two hybrids of Amur mirror carp, who not only achieve high yield of edible parts of the body, but also the earlier tests confirmed higher growth, better survival and resistance to the KHV virus.
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Impact of long-term storage on fish welfare
PECHER, Petr
The literature review is completely described by the issue of fish welfare and whether fish feel pain. Then there are describes the basic procedures for measuring fish welfare. Among which belongs for example a cortisol and glucose in fish blood capillary. The aim of the study was to evaluate the effect of different stress factors during storage on fish welfare and to changes in production, exterior and length and weight indicators. For the experiment was selected carp (Cyprinus carpio) divided into two groups by weight 2.5 kg to 2.5 kg and above. The results are the values of visceral fat, capillary blood lactate and changes in production, exterior and length-weight indicators during the application and the end of the experiment.
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Influence of antiparasitic baths of fish on haematological and biochemical indicators
MECKO, Jozef
The aim of the diploma thesis was to evaluate the influence of antiparasitic baths on selected biochemical and haematological parameters in the blood samples of fish. According to the results the application schema will be specified or amended such alterations will be substantiated. A test was performed on the fry of carp (naked and scaly form) to evaluate the influence of the peractetic acid with the help of biochemical and haematological parameters and with the histopathological examination. The common carp was exposed to the concetration 1 mg?l-1 and 2 mg?l-1 of PAA. Three days after application was taken the blood samples from fish and the biochemical and haematological parameters were determined. Than was taken a sample of skin with the musculature from fish to be sent for the histopathological examination in University of Veterinary and Pharmaceutical Sciences in Brno. Based on the results the naked form of the carp has been declared to be more sensitive to the application of PAA. All the observed changes in haematological and biochemical parameters were within physiological range, so it can be stated, that the application schema of PAA has not a signifiant negative influence on the health of the tested fish.
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Practical verification of rearing of common carp with high levels of omega 3 fatty acids
ZROSTLÍK, Jakub
The aim of my thesis was to verify the technology of rearing common carp (Cyprinius carpio) with increased levels of omega 3 fatty acids. The technology was tested in 2010 and 2011. A mixture of KP Len and cereal was used for the experiment in 2010. In 2011, the fish were divided into three groups The first group was fed a mixture of KP Len, the second a type of cereal, and the third was kept on natural feed. The technology was carried out under the conditions of the Czech fishery industry. During the entire growing period the course of growth was monitored as part of the framework of regular checks and the parameters of the quality of water was measured. The main descriptive characteristics was the fat content and composition of fatty acids. The combination of KP Len achieved a significantly higher content of PUFA than the group of fish receiving cereals in 2010 and in 2011. When comparing groups of fish fed a mixture of KP Len and those fed natural feed in 2011, no statistically significant differences in PUFA were detected. In 2010 the fish fed a mixture of KP Len reached the highest content of EPA and DHA. On the contrary, in 2011 the significantly highest content was detected in the muscle of fish kept on natural feed.
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The influence of carp nutrition (Cyprinus carpio) on changes of meat quality
JANOUŠEK, Petr
The aim of this study was based on laboratory analysis of each parameter, an assessment of livestock interventions, current methods and techniques used in fishing enterprises. Experiments carried out on the market carp in ponds in the system Naděj and the hatcheries under the pond Svět where it was used mechanically and cooked cereals. After processing the fish fillets was done by measuring muscle textural profile. The highest average value of the hardness of the fish ponds was measured in control samples (25,008 ? 3,507 kg). Followed by samples of fish that were unprepared food whatsoever triticale (19,530 ? 2,842 kg). Fish feeding whole triticale heat-treated at 100 ° C (9,990 ? 1,506 kg) and heat-treated triticale mashed at 100 ° C (10,031 ? 1,696 kg) showed similar values of stiffness. The highest average value of the hardness of fish hatcheries was measured in samples of fish that were food whatsoever (20,420 ? 1,456 kg). In addition, followed in descending order of fish samples, which posed a triticale cooked at 100 ° C (11,198 ? 3,334 kg) of wheat at 100 ° C (10,286 ? 1,234 kg), barley at 100 ° C (8,564 ? 1,532 kg), and rye at 100 ° C (7,519 ? 1,993 kg).
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Cryoconservation of fish sperm in model species - common carp (Cyprinus carpio): the influence of different temperature regimes of cryopreservation on the viability of thawed spermatozoa.
SOCHOROVÁ, Denisa
Influence of temperature and freezing rate on sperm survival after thawing were objective of this study. Motility (percentage of moving sperm), velocity and duration of sperm movement before and after process of freezing were observed in common carp (Cyprinus carpio L.) spermatozoa. Solutions recommended by Kopeika (1986) and Kurokura (1984) were used as a cryoprotective media. Sperm freezing was performed in 0.5 ml straws layed in styrofoam box 3, 6 and 9 cm above the level of liquid nitrogen for 20 min. Temperature changes during process of freezing were recorded inside and outside straws using thermocouple thermometer with miniature probes T type (cuprum ? constantan). At a fi rst level (corresponding to height 3 cm above level of liquid nitrogen) we recorded lowest temperature -170 °C, on a second (6 cm) -110 °C and on a third level (9 cm) -70 °C. Best results of sperm motility after freezing ? thawing were achieved using Kopeika solution and freezing at first level (3 cm above liquid nitrogen) where we reported 27 % of motile spermatozoa and velocity of movement 118 ?m.s-1. Freezing by Kurokura solution resulted in motility 14 % and velocity 76 ?m.s-1 whereas motility of native sperm was 88 % and velocity 136 ?m.s-1.
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