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National Repository of Grey Literature

166 records found  41 - 50  jump to record:

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	Nízkoteplotní emisní spektroskopie v ekofyziologii fytoplanktonu Prášil, Ondřej Low temperature (77K) emission spectroscopy is standard techniques of photosynthesis research. Emission spectroscopy provides wealth of information about both photosystems, their pigment composition, relative activity and stoichiometry and has been successfully applied in stress physiology. In this talk I will discuss examples of the use of portable versions of spectrofluorometer. Specifically, I will show that 77K emission spectra of phytoplankton from oligotrophic HNLC areas of subtropical gyres contain information that can be used as marker of iron limitation and provide clues about mechanisms of observed high intrinsic fluorescence yield in these areas Detailed record
	Různé strategie pro rekombinantní produkci Leitner, C. ; Ertl, S. ; Volc, Jindřich ; Haltrich, D. ; Ludwig, R. The fungal enzyme pyranose 2-oxidase (EC 1.1.3.10) is the key catalyst for production of 2-ketoglucose, a glucose derivative of industrial interest. To prevent formation of inclusion bodies during its heterologous expression in E. coli, several fermentation protocols were developed to ensure proper folding and recovery of the enzyme activity at high levels Detailed record
	Expanzi aktivovaných CD8+ t-lymfocytů lze výrazně zvýšit podáváním komplexů IL-2 a anit-IL-2protilátky Tomala, Jakub ; Kovář, Marek ; Strohalm, Jiří ; Etrych, Tomáš ; Ulbrich, Karel ; Říhová, Blanka By using the model of adoptively transferred transgenic CD8+ cells (OT-I) labeled by CFSE dye, we have measured clonal expansion, proliferation and expression of surface markers after various stimulation.We have studies prophylactic effect of complexes, alone or in combination with other cytokines, on various cancer cell lines in vivo. Our results indicate such approach is very promising and could be potent therapeutical tool in tratment of cancer Detailed record
	Ustanovení buněčné nádorové linie, která stabilně exprimuje zelený fluorescenční protein Větvička, David ; Josková, Radka ; Baumgartnerová, E. ; Hovorka, Ondřej ; Ulbrich, Karel ; Říhová, Blanka Murine T-cell lymphoma cell line EL-4 was transfected via the nucleoporation and stable transfectants have been established Detailed record
	Polymerní konjugát doxorubicinu na bázi HPMA, působící ve shodě s protinádorovou imunitou nositele Šírová, Milada ; Rossmann, Pavel ; Mrkvan, Tomáš ; Strohalm, Jiří ; Šubr, Vladimír ; Plocová, Daniela ; Ulbrich, Karel ; Říhová, Blanka Polymeric conjugate Dox-HPMA-HuIg directly targets the tumour due to the EPR effect, it is not detrimental towards the immune system of the host, and acts in concert with the anti-tumour immune responses Detailed record
	Glykosylace povrchové membrány EL-4 buněk: vliv volného nebo N-(2-hydroxypropyl)methacrylamide (HPMA) vázaného doxorubicinu Kovář, Lubomír ; Etrych, Tomáš ; Šubr, Vladimír ; Strohalm, Jiří ; Sklenář, Jan ; Chytil, Petr ; Ulbrich, Karel ; Říhová, Blanka In those experimenst we studied the possible impace of CD43 as highly glycosylated molecule in cells exposed to free doxorubicin (Dox) or to conjugates consisting of HPMA copolymer backbone to which the drug is conjugated via enzymatically (PK1 conjugate) or pH sensitive, hydrolytically cleavable bond (hydrazone conjugate, HYD). Detailed record
	Protilátka anti-DR5 vázaná na HPMA: nový přístup k imunochemoterapii nádorů Říhová, Blanka ; Hovorka, Ondřej ; Kopečková, P. ; Kopeček, J. Dimerized antibodies anti-DR5 bound to the synthetic polymer based on HPMA, effectively induce apoptosis of the tumor cells that express TRAIL on their surface. At the same time, the tumor cells are also targeted by cytotoxic drug +doxorubicin bound to the HPMA polymeric conjugate Detailed record
	Imunostimulační a imunoprotektivní vlastnosti makromolekulárních protinádorových léčiv Říhová, Blanka ; Strohalm, Jiří ; Šírová, Milada ; Etrych, Tomáš ; Mrkvan, Tomáš ; Šubr, Vladimír ; Plocová, Daniela ; Kovář, Marek ; Ulbrich, Karel Polymeric conjugates of doxorubicin, non-targeted or antibody targeted cure mice from experimental EL4 T cell lymphoma and induce specific, systemic long-lasting anti/tumor resistance Detailed record
	Indukce efektivní protinádorové imunity při léčbě směrovaným konjugátem na bázi poly(HPMA) obsahujícím doxorubicin Kovář, Marek ; Tomala, Jakub ; Mrkvan, Tomáš ; Etrych, Tomáš ; Strohalm, Jiří ; Ulbrich, Karel ; Říhová, Blanka Thus, treatment with B1 mAb-targeted conjugates is able to completely cured BCL 1 bearing mice and most importantly, break immunoescaping mechanisms of BCL1 cells Detailed record
	Charakterizace restrikčně-modifikačního systému komensálního kmene Weiserová, Marie ; Ryu, J. We have characterised a putative restriction-modification system EcoA0ORF42P in commensal Escherichia coli strain A0 34/86 (O83: K24: H31). This system is a functional member of the Type IB family, whose specificity differs from those of known Type IB enzymes, as proved by immunological cross-reactivity and complementation assay. Using a plasmid transformation method and RM search computer program, we have identified the DNA recognition sequence of EcoA0ORF42P as GGA(8N)ATGC Detailed record

National Repository of Grey Literature : 166 records found   41 - 50  jump to record:

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