|
Roman Nejedlý and Litomyšl 1871-1901
Nájemník, Václav ; Randák, Jan (advisor) ; Křesťan, Jiří (referee)
Presented thesis inquire into life of teacher, editor and music composer Roman Nejedly. In particular the time period when he worked in Litomysl between 1871 and 1901. Main goal of the thesis was not only to revise factography but also to analyse literature about Roman Nejedly, especially in places, where commentaries were influenced by totalitarian ideology. Roman Nejedly is also father of well known but controversial person of czech history - Zdenek Nejedly. Subject of this study is not just their relationship but also fatherlike effects as positive and negative ideal.
|
|
Functional studies of selected members of the Arabidopsis formin family
Oulehlová, Denisa ; Cvrčková, Fatima (advisor) ; Binarová, Pavla (referee) ; Černý, Jan (referee)
Formins are multidomain proteins containing a conserved formin-homology 2 (FH2) domain, which catalyzes de novo nucleation of actin filaments. In yeast and animal cells, both mechanisms and regulation of formin function have been extensively studied, yet much less is known about action of plant formins, which considerably differ from yeast and animal ones in the domain composition. In higher plants, formins are classified into two groups, Class I and Class II, and so far, experimental data are available only for the first group members. Here I present results of experimental study of several members of the large formin family in Arabidopsis, including the characterization of a Class II formin AtFH16. Arabidopsis genome contains 21 formin-encoding genes, and though they greatly differ in their expression levels and pattern, all of them are transcriptionally active. We selected 17 homozygous T-DNA insertional mutants in 14 formin genes. Under standard cultivation conditions, no obvious phenotypic discrepancies between wild type and mutant plants were found. To impair two dominant pollen formins, an atfh3atfh5 double-mutant was prepared and even in this case, both microspore development and pollen tube growth remained unaffected. Consistently, polarized growth of tobacco pollen tubes was not altered...
|
|
The effects of ions and oxidation on hydration and mobility of model lipid membranes.
Vojtíšková, Alžběta ; Jurkiewicz, Piotr (advisor) ; Michl, Martin (referee)
The presented thesis is a continuation of the bachelor work, in which the effects of monovalent ions on neutral model lipid membranes were characterized. Herein physical properties of physiologically relevant anionic membranes in the presence of monovalent cations and oxidized lipids were studied. Hydration and mobility of the lipid bilayer at glycerol level were investigated using fluorescent solvent relaxation technique. In the first part of this work the interactions of cations (Na+ , K+ , Cs+ ) with negatively charged POPC/POPS lipid mixture, which is a good model of inner leaflet of cellular membrane, were studied. The presence of cations resulted in dehydration and substantial hinderence of mobility of hydrated lipids at the glycerol level probed by Laurdan. Clear specificity of those effects, which correlated with Hofmeister series have been observed. In the second part of the work truncated oxidized phospholipids, oxPLs (PazePC, PoxnoPC, PGPC, POVPC), which are known to be important in pathophysiology of numerous diseases, were investigated. 10 mol% of each oxPL was incorporated into neutral and anionic lipid bilayers, the hydration and mobility of which were measured in water or in KCl solution. The results reveal complex interactions between oxPLs, negatively charged lipids, and K+ . In...
|
|
The effects of ions and oxidation on hydration and mobility of model lipid membranes
Vojtíšková, Alžběta
The presented thesis is a continuation of the bachelor work, in which the effects of monovalent ions on neutral model lipid membranes were characterized. Herein physical properties of physiologically relevant anionic membranes in the presence of monovalent cations and oxidized lipids were studied. Hydration and mobility of the lipid bilayer at glycerol level were investigated using fluorescent solvent relaxation technique. In the first part of this work the interactions of cations (Na+ , K+ , Cs+ ) with negatively charged POPC/POPS lipid mixture, which is a good model of inner leaflet of cellular membrane, were studied. The presence of cations resulted in dehydration and substantial hinderence of mobility of hydrated lipids at the glycerol level probed by Laurdan. Clear specificity of those effects, which correlated with Hofmeister series have been observed. In the second part of the work truncated oxidized phospholipids, oxPLs (PazePC, PoxnoPC, PGPC, POVPC), which are known to be important in pathophysiology of numerous diseases, were investigated. 10 mol% of each oxPL was incorporated into neutral and anionic lipid bilayers, the hydration and mobility of which were measured in water or in KCl solution. The results reveal complex interactions between oxPLs, negatively charged lipids, and K+ . In...
|
|
Determinants of the circulatory system diseases among the Czech population
Lustigová, Michala
Determinants of circulatory system diseases among the Czech population Abstract The determinants of circulatory system diseases are very well known, modifiable risk factors and factors widely spread among populations are in focus for the public health research. The aim of this thesis is a quantification of main cardiovascular risk factors in the Czech population using the survival analysis and HAPIEE cohort data. The epidemiologic situation, trends in the Czech population health including "the cardiovascular revolution" is discussed in the first part of the thesis. Among the Czech population high prevalence of many risk factors were found. The education had the strongest impact on cardiovascular health from sociodemographic characteristics. The negative effect of smoking, prevalence of high blood pressure, prevalence of diabetes and physical inactivity was confirmed. On the other hand the impact of obesity, binge drinking and high blood cholesterol level was not significant among the Czech population. The population approach in cardiovascular epidemiology and cardiovascular health promotion as well are discussed in the last chapter. Keywords: diseases of the circulatory system, mortality, health transition, cardiovascular health, risk factors, survival analysis, health promotion
|
|
Symbolické reprezentace kompaktních prostorů
Kazda, Alexandr
Title: Symbolic representations of compact spaces Author: Alexandr Kazda Department: Department of Algebra Supervisor: Prof. RNDr. Petr Kůrka, CSc. Supervisor's e-mail address: kurka@cts.cuni.cz Abstract: The thesis concerns itself with Möbius number systems. These systems represent points using sequences of Möbius transformations. We are mainly inter- ested in representing the unit circle (which is equivalent to representing R ∪ {∞}). The main aim of the thesis is to improve already known tools for proving that a given subshift-iterative system pair is in fact a Möbius number system. We also study the existence problem: How to describe iterative systems resp. subshifts for which there exists a subshift resp. iterative system such that the resulting pair forms a Möbius number system. While we were unable to provide a complete answer to this question, we present both positive and negative partial results. As Möbius number systems are also subshifts, we can ask when a given Möbius number system is sofic. We give this problem a short treatment at the end of our thesis. Keywords: Möbius transformation, numeral system, subshift
|
|
Biological activity of plant metabolites IX. Antimicrobial activity of some secondary metabolites
Váňová, Jana ; Opletal, Lubomír (advisor) ; Karlíčková, Jana (referee)
Váňová, J.: Biological Activity of Plant Metabolites IX. Antimicrobial Activity of Some Secondary Metabolites. Diploma Thesis, Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmaceutical Botany and Ecology, Hradec Králové 2008, 62 pp. The antibacterial activity of 4 selected plant extracts was evaluated against E. coli O157:H7 and Staphylococcus aureus. Extracts were prepared by extraction in 80% ethanol and subsequent percolation. Two methods were used for determination of minimal inhibitory concentration (MIC). In the first case they were not reached the values of MIC expected for E .coli on the basis of the other literature studies. All samples within the concentration range of 5 - 0,005 ppm which were prepared in two series were negative even after 48 hours exposition. In the second case it was reached better results for E. coli and S. aureus. Two series of serial twofold dilutions within the concentration range of 25 %-0.02 % were prepared. The lowest values of MIC were exhibited by Thymus vulgaris extract, especially against Gram-positive indicator strain. Nevertheless the values of MIC remain very high (3.13 % for E. coli, 0.2 % for S. aureus). For determination of minimal bactericidal concentration (MBC) in E. coli O157:H7 and E. coli O55 seven...
|
|
Statistical inference for random processes
Kvitkovičová, Andrea ; Štěpán, Josef (referee) ; Hlubinka, Daniel (advisor)
The thesis deals with testing hypotheses about the parameters of the Wiener process with a constant drift rate and instantaneous variance. The tests are based on the first time, when the process reaches a pre-specified boundary point. We consider a process with a non-negative drift rate, and we observe hitting a positive point. We focus on tests about the drift rate, in particular about the absence of any drift. We first study several basic properties of the Wiener process and its connection with the Wiener process with a drift. Using these, we derive distributional properties of the first hitting time. We also describe selected hypotheses testing techniques in the setting of exponential families. We construct uniformly most powerful unbiased tests of one parameter in the presence of a nuisance parameter. Further, we construct uniformly most powerful tests of hypotheses about the drift rate, while the variance is known, and we study this situation in more detail. Finally, we construct asymptotic simultaneous tests of both parameters based on the R'enyi divergences.
|
| |
|
Learning Restricted Restarting Automata using Genetic Algorithm
Basovník, Stanislav ; Plátek, Martin (referee) ; Mráz, František (advisor)
Restarting automata are linguistically motivated models for language representation. The main goal of this work is to propose a suitable version of restarting automaton for learning from positive and negative samples using genetic algorithms. We also characterize the class of languages accepted by limited context restarting automata with respect to the Chomsky hierarchy. The proposed learning algorithm is compared to two well-known methods for learning languages from positive and negative samples - RPNI and LARS. A tool for learning the restricted version of restarting automaton is developed as a part of this work. Examples of usage and user guide are included in this work.
|
guest ::
