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History of critical and intensive care - past and present
HOVORKOVÁ, Nicole
The title of the bachelor thesis is "History of critical and intensive care - past and present". Its main aim is to describe its origins in order to explore the history of providing critical and intensive care, both in the Czech Republic and in a global context, and also in comparison to the current state of the medical field. This work is focused on the period of development of intensive care, from the 50s to the 80s. It is a theoretical work done by detailed research of secondary and primary sources. It is divided into two parts. The first is dedicated to intensive care and its global development and in the Czech Republic. The second part contains data about intensive care within the hospitals of South Bohemia, which I have gathered during the study of materials provided by the local archives. The first chapter and the second chapter is dedicated to the history of nursing, both worldwide and in the Czech Republic. It serves for better orientation in the next chapter. The third chapter deals with intensive care itself. It is described in many of its aspects and in its historical and global context. The fourth chapter brings us to the second part of the thesis, which is supplemented with information from archival materials found during the research in regional archives. The thesis includes some photos that were captured either during a visit to the Museum of the National Medical Library, or during visits to the archives.

Comparison of intercultural competences of nursing staff in the Czech Republic and German-speaking areas
TÝLEOVÁ, Tereza
The increasing migration of population in the 21 century causes development of multicultural societies. With the increasing number of foreigners in individual countries it is possible to also presume an increasing number of patients/clients from different cultures in medical facilities. These facts require modern attitude to nursing, developing of transcultural nursing, implementing multi- and transcultural education in the health care education and propagation of multicultural conception of nursing between the nursing personnel. The result of these necessary changes should be the ability to provide effective nursing care which is culturally adjusted to the patient/client that would also take their specifics, customs and traditions of their culture into account and that would saturate all their biological, psychological, social and spiritual needs according to their race, nationality or belief. This thesis deals with multicultural nursing and confronts cultural competence of the nursing staff in Czech Republic with that in German-speaking countries. This thesis aim is to state the level of multicultural competence of the nursing staff and explore conditions provided to the nursing personnel of the hospital for giving culturally adapted care to patients-foreigners. The results of the research give an overview of the state of culturally competent care in comparable medical facilities in Czech Republic, Germany, Austria and Switzerland. They will also give a feedback to the questioned hospitals. Such information may alert their management to possible lack of knowledge of their nursing personnel and help them direct its further education. The theoretical part of the theses defines the basic concepts regarding culture, ethnic groups, race or nation, formulates the difference between the terms "multicultural" and "transcultural" nursing, multicultural education and culturally competent care, compile the most common sections of specific cultural needs of the individuals. In short it also processes J. Campinha-Bacote's model of cultural competence in health care delivery, the Papadopoulos, Tilki and Taylor Model for developing cultural competence and the Purnell Model for cultural competence. It also shortly deals with nursing frameworks for acquiring cultural data, such as the Sunrise model from M. Leininger, the Giger and Davidhizar Transcultural assessment model and Bloch's ethnic/cultural assessment guide. The empirical part processes and evaluates data obtained during the quantitative research. The accumulation of the data was conducted through anonymous questionnaires that were distributed between the nursing personnel of internal and surgical wards of the hospitals in Czech Republic and in German-speaking countries. In Czech Republic these were hospitals in Prague and in Tábor, in Germany a hospital in Dortmund and one in Cottbus, in Austria the hospitals in Salzburg and in Weiz and in Switzerland a hospital in Aarau and one in St. Gallen. The collection of the data was conducted either on-line or by way of printed questionnaire forms. The acquired data were valorized and sorted into diagrams and tables for further clarity. From the respondent´s answers follows that the queried medical facilities in Czech Republic do not provide sufficient conditions for their workers to give culturally competent nursing care on such a level, as it is at the facilities questioned in the German-speaking areas. The results also show that language skills of the nursing personnel in these hospitals in Czech Republic are worse than those in the German-speaking countries. With the level of multicultural competence the situation is similar; in this field Czech nursing personnel does not match their foreign counterpart. It is necessary to remark that this research sample of two hospitals from each of the countries is insufficient for the results to be applied on the whole Czech Republic or German speaking countries.

Coping strategies in children´s fear and pain in relation to nursing care
PROCHÁZKOVÁ, Zuzana
For the nurse, taking care of hospitalized children is important not only pediatric nursing knowledge, personal and social preconditions, but also the theoretical and practical competency of methods and techniques, which are used for identification and effective influencing of pain and fear. The aim of this thesis was to identify the main sources of fear of the hospitalized preschool and young school age children and to find out the effective methods and principles of the cooperation with children, alternatively with their accompanying parents in order to moderate the fear of the small patient. Then the thesis tried to chart the nursing diagnostic and evaluation procedure of the pain and get the most used nursing methods of non-pharmacological pain relief. To obtain the necessary data, the qualitative research was used with a semi-structured interview as a research instrument. The first research group consisted of 12 nurses, the second group consisted of 12 children ´s patients of preschool and young school age. The majority of the asked nurses thinks, that children´s patients are afraid especially of non-familiar environment, then of the awaited pain and of the solitude without parents. However, the most patients said, that they were afraid especially of pain, then of non-familiar environment, restriction of their needs, hostile attitude of the medical staff and of the other neighbouring patients. They were also frightened of the death and of the darkness. Most reported nurses mean, that the most effective solution of the children ´s fear is the friendly attitude of the medics. They also mentioned the fact of taking the focus off the fear and the presence of the parents is also beneficial. The nurses ´ answers were in conformity with patients opinion in two fields - friendly attitude of the medics and taking the focus off the fear. Then the children mentioned abiding of their specifics in connection to falling asleep and the demand of beying treated by the same sister or by few same sisters. All respondents also talked about the benefit of parents presence. Regarding the principles of the cooperation, nurses answered the importance of united medical procedures, especially the proper way of information transfer. Then the respondents nurses mentioned the psychical and emotional support for children and their accompaniments, the atmosphere of confidence and the value of well done education. Also the need of calm, silence and privacy was stated. Unlike nurses, children and their parents regarding to principles of the cooperation prefer the psychical and emotional support, then the importance of enough information was mentioned. The fourth research question bears on the methods used for children pain monitoring. The nurses said, that the intentional interview with the patients or with the parents was used as a main technique, that was stated by patients as well. Research question number five said: What are the most used nursing methods of non-pharmacological pain relief at children? Most responded nurses mentioned the distraction of children attention from the painful incentive, then the importance of physical methods, relief position and purposeful preparation for the unpleasant intervention. Nurses also named the helpful contribution of the parents, relaxing exercises and the method of imagination. Regarding the pain treatment, it was noted, that all patients prefered the analgesic therapy. Then they quoted the distraction of their attention from the painful incentive, the friendly attitude of the medics, using the ice compress and the relief position. In the pain acute phase, children required mostly calm, comfort and privacy. All children also considered the presence of parents as very helpful. The theoretical and empirical part of the thesis are suitable for being studied by nurses taking care of children and it can be helpful for nursing quality improvement at children ´s wards in hospitals.

Role of physical activity in the prevention of childhood obesity
VRTALOVÁ, Jana
The thesis deals with physical activity in the prevention of childhood obesity. It is focused on children, namely pupils of the eighth and ninith grades of elementary school. Obesity is responsible for more human lives than both of the world wars and if we want this epidemic of obesity and problems associated with inactivity minimized, we have to start where it is most efficient and that is with children. The main objective is to explore physical activity in a selected group of pupils. Partial aim is to determine the prevalence of overweight and obesity in the investigated group of students using somatometric measurements in the selected location. Another objective is to determine how many of childrens parents did sports in the past or actualy do sports. The final goal is to prepare educational material for students about the importance of physical activity. The work is divided into two parts theoretical and practical. The theoretical part was compiled as a secondary data analysis. Sources of data and information originated from the Czech Republic and abroad in the form of scholarly books, journals and electronic resources. The contents of the theoretical part is focused on explenation of terms such as obesity, its consequences, complications, ways to prevent obesity, physical activity, appropriate kinds of physical activity and its effects on the human body. Distinctive part is the chapter dealing with the myths of obesity some of them are really surprising. The second part is practical part and includes its own research. The investigation was conducted in a primary school Komenského alej in Žatec for pupils of the eighth and ninth grades. File featured 107 pupils. The research was conducted as a quantitative survey complemented by measurements of somatometric indicators. The data were collected using a custom non-standardized questionnaire. The questionnaire contained 14 questions and two columns separated by line for recording the exact weight and height. We checked these data at pre-agreed periods.

Účinky xenobiotik na oxidační stres, metabolizmus lipidů, integritu DNA a životaschopnost lidských buněk a rybích spermií in vitro
LINHARTOVÁ, Pavla
Pollution of the aquatic environment by inorganic and organic chemicals is a major factor posing a serious threat to the survival of aquatic organisms including fish. In addition balancing risks and benefits of fish consumption is nowadays an intensively discussed public health topic. Spermatozoa of almost all fish species are released into water environment where they can be directly exposed to various compounds, such as xenobiotics including toxic metals, prior to fertilization. In addition, exposure of parental adults to various xenobiotics may affect gamete quality, which may subsequently reduce fertilization success. On the other hand the advantages of eating fish are well-known, not only in the point that fish is a healthy source of protein and other nutrients, but eating contaminated fish may also confer various health benefits. Research over the past few decades has shown that the nutrients and particularly the n-3 fatty acids (FA) found in fish and seafood, are for examples protective against cardiac diseases and have a positive impact on brain development. The thesis provides a focus on two different cell model types. Firstly, human hepatocellular cells (Hep G2, ATCC) were used as in vitro tool for studying the effect of the intake of cadmium (Cd2+) contaminated fish on cytotoxicity, oxidative stress and fatty acid and phospholipid class compositions. Secondly, spermatozoa of one threatened species of fish, sterlet (Acipenser ruthenus) were used as in vitro model for studying effect of potentially hazardous xenobiotic compounds' occurring in open waters. Sperm from sterlet were exposed for 2h to environmentally relevant concentrations of DQ (0-150

Nové postupy biomonitoringu cizorodých látek ve vodním prostředí
ČERVENÝ, Daniel
Within the presented work, several approaches of biomonitoring were studied. A well-established method for human health risk assessment was applied to evaluate the quality of fish from open waters in the Czech Republic. As this work is targeted on sport fishermen, who frequently consume their own catches, besides the publication in scientific journal, a brochure with results of this study was distributed via the Czech Fishing Union. Health risks for consumers related to the consumption of wild fish were found strongly species and locality dependent. As there is no risks related with the consumption of carp (Cyprinus carpio) at all investigated sites, frequent consumption of predatory fish should be avoided at some fishing grounds. The use of passive samplers has become more and more attractive in the last two decades. As these devices are able to mimic the biological uptake of chemicals, their potential for replacing fish as bioindicators in routine biomonitoring programmes is evident. In our study, the comparison between analysis of fish tissues and POCIS extracts from selected sampling sites confirms this idea about interchangeability of these indicators in the case of PFASs as target pollutants. Moreover, no metabolic transformation of contaminants present in water take place in passive samplers contrary to living organisms, thus these reflect the actual contamination at the locality more precisely. The use of passive samplers completely fulfils the internationally accepted principles of Replacement, Reduction, Refinement (the three R´s). Another promising approach using YOY fish as bioindicators was evaluated within the present study. Multispecies samples of YOY fish homogenate was found as a better indicator for wide range of pollutants than muscle tissue of adult fish within one species from the same locality. As all inner organs and tissues are presented in analysed samples, more target pollutants in higher concentrations are detected compared to the muscle of adults. Besides the higher sensitivity in pollutants detection and quantification, practical and economic benefits are of concern in the case of the YOY fish approach. The YOY fish approach also particularly fulfil the three R´s strategy, as sampling of this age group of fish has a smaller impact on the environment than the sampling of adults. In case of mercury, that was found as a limiting contaminant for the consumption of fish from open waters in the Czech Republic, a nonlethal method of finclips sampling can be used for the monitoring of this compound in aquatic environments. Besides the monitoring, our optimised method for the estimation of muscle tissue concentrations could be used for human health risk assessment as well. The difference between real muscle concentrations and concentrations estimated using our method differs less than 10% in most of the investigated localities. Benthic organisms are an important part of the food web in aquatic environments, but insufficient information about its contamination is available. In another study included in this thesis, first evidence about bioaccumulation of certain pharmaceuticals in benthic organisms was described. Although, pharmaceuticals are generally considered to be not accumulative in organisms, our study proved that certain pharmaceuticals have bioaccumulation potential. From this finding, it is evident that not only the concentrations of pharmaceutical dissolved in water but also the diet represents an important exposure pathway of contamination by these compounds for organisms (fish) inhabiting aquatic environments.

Summary of foreign knowledge about the origin and development of EDZ in crystalline rocks - research
Vavro, Martin ; Souček, Kamil ; Staš, Lubomír ; Vavro, Leona
Presented search summarizes findings of foreign research oriented on the origin and evolution of the excavation damaged zone in crystalline rocks with a particular focus on the essential results of experimental projects which were performed in Canada, Sweden, Finland, and Switzerland. The study is divided, excluding the introduction, into seven main chapters of the text, which gradually deal with: (1) definition of key terms, (2) overview of the main underground research laboratories in the world where EDZ assessment was conducted, (3) methods suitable for EDZ description and characterization, (4) main factors influencing the origin of failure around the excavations and time-dependent evolution of EDZ. An overview of important outcomes of EDZ experiments, focusing on the European hard rock laboratories (Stripa, Äspö, Onkalo/Olkiluoto and Grimsel), and their summary are presented in the final two chapters.\nThe review summarizing the published key findings and results of in situ experiments shows, that for rock in lower stress state, i.e. in no spalling environment, the extent and character of rock mass damage is typically dependent on the excavation method. Using mechanical excavation, rock damage zone with thickness less than 3 centimeters can be originated. The microcracks within this zone contribute to the increase of hydraulic conductivity of the rock mass. At some test sites (Äspö, Grimsel), where the tunnel boring machine technology was used, the damage zone was already detected in depth of less than 5 mm.\nOn the contrary, openings excavated by drilling and blasting are characterized by much more extensive damage zones up to several tens of centimeters in width. The damage progressively diminishes with the distance from the opening.

Comparison of HPLC a GC-MS method for pesticide determination in soil
Chalbia Václavíková, Alena ; Kočárek, Martin (advisor) ; Havlík, Jaroslav (referee)
Physical and chemical properties of pesticides may vary significantly. Pesticides can be acidic, neutral or alkaline. Some compounds containing phosphorus, other sulfur, nitrogen or halogens. These atoms can be important for the detection of pesticides. Some of the compounds are volatile, others on the contrary not evaporate, which is also one of the important properties for chromatography. The diversity of pesticides actually makes it impossible to create a universal method, which is very impractical in terms of quality and time determination. Because that pesticides are one of the most chemicals in most countries. In the food and drinking water are limits on pesticides in order to avoid negative impact on public health. For residues in soil or surface water limits are set. (Lutz et al., 2006) The objective of this thesis is to evaluate the possibility of using two types of chromatographs with different detectors for the analysis of pesticide. Pendimethalin which was chosen for this work, will be analyzed usinga liquid chromatograph with UV detektor and gas chromatography with mass detector. Both this method will be compared as well two extraction methods (Soxhlet extraction and shaking), and two extraction agents (methanol and acetonitrile). Another point of the work is to provethe hypothesis that, for the determination of pendimethalin in soil is by gas chromatography using a mass spectrometer in comparison with the high performance liquid chromatography time and more expensive, but will achieve a higher yield and a lower detection limit of the pesticide.

Local Government and Public Life in the Municipality of Hostouň
Husak, Vladimír ; Čopík, Jan (advisor) ; Kobzev Kotásková, Sylvie (referee)
The bachelor thesis is dedicated to local government and community life in the village Hostouň. The goal is to characterize the local government, local organizations and their influence on public life in the village. The work is divided into two main parts. The theoretical part defines the basic structure of public administration and lawful functioning of the local government. The practical part describes the basic characteristics of the village. Attention is paid to the historical, cultural, demographic and sociological characteristics of the village. Municipal elections and financial characteristics of the village are also being addressed. Last but not least it also deals with the social and political life in the village, in which the local associations play a big role.

Effect of water deficit on the energy content of individual plant organs in selected species of leafy vegetables
Šimůnková, Petra ; Hnilička, František (advisor) ; Česká, Jana (referee)
This work presents the impact of water deficit on dry mass production and photosynthetic accumulation of energy on every single plant´s organs of selected plant species. As a chosen experimental material were taken plants of Spinacea oleracea "Matador" sort and Tetragonia tetragonioides. Spinacea oleracea and Tetragonia tetragonioides plants were grown in containers 11x11cm large in mixture of garden substrate A with silicon sand (2:1) in 2 variations placed in FAPPZ greenhouses. One variation was for control and second was exposed to stress reaction. Control variation had been watered for whole time of experiment with 50ml of water 2 times per week. Stressed one had been watered after puncturing and 6. taking until the end of experiment. During the plant´s ontogenetic evolution had been monitored dry mass´s weight on singular plant´s organs. With usage of burnt calorimetry method had been monitored capacity of photosynteticaly accumulated energy in dry mass. Quantity of netto energy had been found out by dry burning adiabatic calorimeter LAGEST MS 10A. From the gained results emerge, that plants of Tetragonia tetragonioides have 2 times higher weight growth apart from Spinacea oleracea. Control plants of Tetragonia tetragonioides have higher weight by 25,5% (123 mg) compared to the plants growing in water deficit. Likewise higher weight of roots had been observed on control plants (41 mg) compared to the stressed plants (40 g). Control spinach plants had higher weight of above-ground biomass by 19,5% compared to the stressed ones (43 mg). Roots of control plants had lower weight compared to stressed variant by 2960 mg. From the results emerge, that plants of Tetragonia tetragonioides have higher weight of roots dry mass and above-ground biomass compared to the Spinacea oleracea plants. Burning heat without ashes was on above-ground biomass of control plants Tetragonia tetragonioide on 15,96 kJ.g-1 level and on stressed plants 14,93 kJ.g-1 . In case of Spinacea oleracea plants was amount of control plants energy 14,98 kJ.g-1 and on stressed 14,21 kJ.g-1. Burnt heat without root ashes of Tetragonia tetragonioides control plants was higher by 1.11 kJ.g-1 then on stressed ones (13,19 kJ.g-1). In case of Spinacea oleracea plants was amount of roots energy on control plants 11,90 kJ.g-1 and on stressed 11,72 kJ.g-1 . Based on measurement were verified differences between species in reaction on water deficit and observed characteristic. From measuring emerged, that Spinacea oleracea plants are more sensitive on effect of water deficit compared to the Tetragonia tetragonioides plants, which is more tolerant. Next thing observed is, that there are differences in amount of dry mass and rate of burnt heat in relation on effect of water deficit.