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Vývoj metodologické a technologické platformy pro neinvazivní odhad fenolických látek v listech a bobulích
ŠEBELA, David
Plant optical signals can serve as important source of information about biochemical and physiological processes in plants. These signals are influenced by compounds synthesized by plants during primary or secondary metabolism and thus, can also serve as their qualitative and quantitative indicators. Light reaching plant surface (leaf or fruit) can undergo three main pathways- it can be (i) reflected, (ii) absorbed or it can (iii) transmit through plant material. The probability of these three processes depends on particular wavelength of incident irradiation and on the morphological characteristics of plant tissues themselves. As such, plant contains various spectrum of photosynthetic pigments and fluorescent compounds which can either reflect, absorb or pass incident irradiation through at specific wavelengths. Biophysical techniques working with these optical properties of plant pigments and/or other compounds have become universal and common tool in basic and applied research. To quote some example, chlorophyll fluorescence imaging, UV induced fluorescence or spectroscopic techniques are on the top of interest thanks to its non-invasive nature, allowing maintain the integrity of measured cells or the whole plant constituents. The main aim of this thesis is to provide a comprehensive study on the possibility of non-invasive monitoring of phenolic compounds in the leaves and fruits.
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Fast food consumer´s awareness about the health risks and factors leading to preference for this type of meals
BENÝŠKOVÁ, Ivetta
Consumption of fast-food style meals increases the risk of occurrence of health problems that might result in some diseases called lifestyle diseases or diseases of civilization. Frequent fast food consumption and related inadequate eating habits lead to increased morbidity and mortality linked particularly to obesity, diabetes, hypertension, cardiovascular diseases and other health problems even from early age. This thesis analyses the problems of eating at fast food restaurants and stands from the point of view of health, proper nutrition and eating habits. The aim of the thesis is to describe objective health risks resulting from regular fast food consumption. Nutritional components and values of fast food meals and their influence on health were described as well as possible risks of development of some lifestyle diseases. The aim of the practical part of the thesis was to map the awareness of health risks resulting from frequent consumption of this kind of food among fast food consumers and the factors that lead them to preference, choice of this kind of nutrition instead of rational nutrition. The research was performed by means of qualitative data collection methods observation of the target group consisting of visitors of fast food restaurants and stands and the method of structured interview with selected fast food consumers. The research was performed with fifteen respondents selected by random selection method. The data collection results were subject to content analysis. The results have shown that Czech fast food consumers are informed on health risks like obesity, cardiovascular diseases, diabetes, hypercholesterolemia, hypertension and gastrointestinal problems caused by frequent fast food consumption and they are aware of the negative nutritional aspects of this type of diet, particularly high content of fat, salt and sugar. The research result have also shown that Czech fast food consumers do not prefer fast food to rational nourishment. If they eat fast food, it is because of haste and lack of time and when they feel they have no other possibility. The consumers prefer fast food when they have real appetite or when they are in a foreign town or country. Social factors have also proven to have influence on fast food consumption. Eating at fast food restaurants with friends is particularly popular among teenagers and children, who even go to fast food restaurants to play, celebrate birthdays etc. The observation results have shown that location of fast food operations have no essential influence on attendance, type of consumers and the factors affecting consumption of this kind of food.
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The Education Systems and Competencies of paramedics in the Czech Republic and in the Federal Republic of Germany
ZBOŽÍNEK, Jonáš
This bachelor thesis is devoted to the paramedic education systems in the Czech Republic and the Federal Republic of Germany, and to comparing the competences these professions have. The theoretical chapter is divided into two sections. The first part focuses on the paramedic education system in the Czech Republic. A brief description of the specialisation is followed by the possibilities of obtaining the qualifications of a paramedic. To illustrate these possibilities, the first stage of university education and its accredited subject A Paramedic Education Programme offered by the University of South Bohemia in České Budějovice serves as a model example.The competences of paramedics in the Czech Republic are based on the valid regulation of the Ministry of Health no. 55/2011 of the Collection of Laws on the activities of non-doctor health-care workers and other specialised personnel. This outline includes also a list of the possibilities of deployment of paramedics in the Czech Republic and their brief descriptions. This part is brought to a close with an account of the possibilities of further education and specialisation. The second part of the theoretical chapter concentrates on the education system for the ambulance service personnel in the Federal Republic of Germany. Individual subchapters describe the levels of non-doctor health-care workers and their activities in the ambulance service. In the German ambulance service, the most corresponding counterpart of the Czech paramedic is the paramedic assistant (to be replaced by emergency attendant due to the German legal regulation that came into force on January 1, 2014). In this section, the main attention is devoted to the education of paramedic assistants. The practical part of the bachelor thesis employs the method of qualitative research through structured interviews with open-ended questions. The sample respondents included men and women working with the ambulance service in České Budějovice and having the qualifications of a paramedic, and for the German part paramedic assistants from the ambulance crew in Passau. To answer the first research question Do the Czech paramedics have wider competences than the German ones? it was necessary to compare the legal regulations in both countries and the appropriate expert literature. The data thus obtained have been checked through the direct questioning of paramedics in both countries. The results show that in comparison with the German paramedic assistant, the Czech paramedic has a wider scope of activities in health-care, even outside the ambulance service. Czech paramedics can work at anaesthesiology & resuscitation departments, at accident & emergency departments or as emergency call operators. Unlike in Germany, all these possibilities are specified also in the law. The comparative analysis therefore shows that the Czech paramedic has overall wider competences than the German paramedic assistant, even though in the sphere of pre-hospital emergency care their competences are very similar, if not fully identical. The second research question was whether it would be beneficial for the emergency assistants in Germany to study their specialisation at university. The analysis of the structured interviews with emergency assistants resulted in the conclusion that they would not be in favour of such an arrangement. In their opinion a university education programme would mean an unecessary complication due to the higher financial costs of German university studies. Not all people interested in this specialisation could afford to study it. The respondents also pointed out that it would be impossible for the current health-care system to pay these university-educated specialists. They voiced some criticism of the present-day education system, nevertheless they hope that the long-overdue expected legislative changes will mean improvements of all the criticised issues.
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The contributions of the sections (NACE-CZ) to the creation of gross value added
BEDNÁŘOVÁ, Monika
The aim of this thesis was to evaluate the contributions of the sections (NACE-CZ) to the creation of gross value added. The first part of this thesis described the theoretical concepts relating to national economic gross value added. Analytical processes were used for the calculations, which may be used only if we are dealing with an additive link between individual factors. The sections' contributions to the creation of national eco-nomic gross value added were evaluated in the practical part, on the basis of the proc-esses set forth in the methodology. In the given time horizon, contributions by institu-tional sectors and groups of sections classified according to the level of technology showed a certain dependency on the actual economic cycle. Although the strongest in-stitutional sector is non-financial enterprises, they were the ones most affected during the crisis period, together with government institutions. On the contrary, the financial institution sector showed a strong position during the crisis period. In terms of the grouping of the sections according to the level of technology, the greatest contribution to national economic gross value added is by groups B1 and B2. The influence of the economic cycle was noted in all the groups but, according to the results, group C did not react quite as sensitively as the other groups.
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Injuries of children at home
POCHYLÁ, Petra
The theoretical part of this thesis is oriented at an actual issue of accidents involving children in home environment, their prevention and a correct provision of first aid by a non-professional at these types of selected accidents. The practical part of this thesis was led by a qualitative form of research, a method of a semicontrolled interview. The interview given was led by chosen caring people, who gave first aid to children with a home environment injury. The whole process of the interview was recorded at a digital record book and then rewritten to a text form, with the approval of the respondents. The respondents were chosen on the basis of a survey. The final information from the interviews were analyzed and subsequently summarized into results. Six respondents were registered into the research part. Six basic questions were given to the respondents. The questions were chosen to find out information about an injured child, an injury character, a procedure of caring person while giving first aid, or other professional care and permanent effects. While preparing the research part, I was expecting that most of the respondents would manage to give first aid, however, according to the results, half of the parents didn't know how to deal with giving first aid. Non- professional first aid, provided at the right time and done correctly, is one of the basic and essential steps to reverse the injured person back into the normal quality of life. This is why, all people should be able to give first aid without special equipment and tools. The aim of my thesis was to monitor the process of the caring person while giving first aid for selected accidents involving children in home environment. I gave myself one fundamental research question: What was the process of the caring person while giving first aid for accidents involving children in home environment? At the final evaluation and comparison of my results, I achieved the results, in most examined facts, which are presented in other different studies dealing with similar issues- children's injuries and giving first aid for accidents involving children.
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THE DRAMATICAL SCRIPT AS A NECESSARY BASIS FOR SPACIAL REALIZATOIN
Tretiag, Štěpán ; KORČÁK, Jakub (advisor) ; HRBEK, Daniel (referee)
Using the concrete examples of my practical bachelor work my intent is to retrospectively describe and reflect on our scenic realization of the play Missing by south-African author Reza de Wet. The essence of a modern dramatic direction is to perceive the script of a play as a model which becomes a basis for its interpretation and the subsequent staging of the play. It is the process from the initial interpretation to final scenic realization I wish to focus on in my work.In the first part I will be dealing with creation of a physical space, that being stage design. I will try to capture what lead us to its final shape and how the basis for that can be found in the script of the play as well as the potential capability of the physical space to become a dramatic space. Its ability to create a platform for the actors to proceed from and how can it provoke them into acting, in particular. In the second part I will be dealing with the dramatic space, ergo the spacial expression of relationships and themes. I will proceed again retrospectively, focusing on how we managed to achieve the stage image which is functioning as a materialized metaphor. I will focus mostly on non-verbal actions, but with regard to the fact, that dramatical script is the basis for our realization of the play.
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The Importance of the Fundraiser in a Non-profit Organization
STARÁ, Radka
The main theme of the bachelor work is the importance of the fundraiser in a non-profit organization. It defines the term fundraising, its principles, forms and methods, the work of a fundraiser in a non-profit organization.The work characterizes nonprofit organizations, their legal status and field of activity. There are also information about activities, which improve economy and financial situation of these organizations. In the practical part of my work I have analyzed the way of financing a non-profit organization Junak- Czech scout, Centre Pod Kleti Holubov z.s. I am comparing in this part the way of fundraiser work on the local and state platform. The facts here available are drawn from the public web pages of this organization and also from the account keeping documents of its local center. At the conclusion of my work I am assessing the effectiveness of fund raising plan and its strategy and also describing the way of negotiation with donors. I am noticing the need of a fund raising team creation and also rules of fundraising including necessary cooperation between the center of organization and its local branches.
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Estrogen receptor beta (ERβ) in testicular cells and sperm
Dostálová, Pavla ; Žatecká, Eva ; Děd, Lukáš ; Dorosh, Andriy ; Postlerová, Pavla ; Jonáková, Věra ; Dvořáková-Hortová, Kateřina ; Pěknicová, Jana
Estrogen is a steroid hormone that plays an important role during sperm development in the male and female reproductive tract. Estrogen signalling is a complex process that depends on cell milieu and presence of receptors. Thanks to the steroid nature of estrogens, they can pass through the plasmatic membrane and bind to the intracellular estrogen receptors (ERs). Within the cell, there are several pools of ERs. One of them is localized to the cell nucleus and their activation leads to direct or indirect binding to DNA and ultimately to alternation in gene expression (genomic pathway). Other pools of ERs are associated with plasma membrane or are located in cytosol. Activation of membrane associated ERs leads to rapid non-genomic responses. Nowadays, two classical estrogen receptors are known – ERα and ERβ. Since ERβ is a predominant variant in testes, we focused our study on expression of ERβ variants in murine testes and sperm. We detected two variants of ERβ at mRNA level in both, testes and sperm. These variants differ in 54 nucleotids within the ligand binding domain and this variability results in different affinity to estrogen. We analyzed individual testicular cell types (spermatogonia, spermatocytes, spermatids, Sertoli cells) by RT-qPCR. Our results suggest that both ERβ variants are coexpressed in the same cell type and may therefore interact together. This may have consequences in mediating of estrogen signalling. Moreover, ERβ is expressed more in the later stages of spermatogenesis suggesting the role of ERβ in these stages or alternatively in spermatozoa alone. At the protein level, we detected ERβ in nuclear, membrane and cytosolic fraction prepared from testicular tissue suggesting the involvement of both, genomic and non-genomic, pathways of estrogen signaling in testes. In sperm, anti-ERβ antibodies localized ERβ in acrosome region and tail which is in accordance with the known role of estrogen on capacitation, acrosome reaction and motility.
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The role of a nurse in a prevention and a treatment of intestinal parasites of children
JANDOVÁ, Anna
The best-known intestinal parasites are Pinworms, Roundworms, Tapeworms and the disease called Toxocarosis. The oftenest parasite is, according to sources, the Pinworm. Intestinal parasites affect mostly young children of pre-school age, sometimes even older. The first goal of the thesis was to investigate parents' awareness of the prevention of parasitic diseases in children. There is a hypothesis set to this goal: Parents of children who suffered from parasitic disease are more informed than parents of children who have not had this disease. The second goal was to map the specifics of nursing care for children with parasitic diseases at GPCHA practice. A research question was chosen for this aim: What are the specifics of nursing care at GPCHA in parasitic diseases? In methodology, the empirical part was chosen and it was a qualitative - quantitative research survey. For the quantitative part, the interrogation method and technique of non-standardized questionnaire was used. The research sample of quantitative survey consisted of 223 respondents, it means parents whose child is aged from 0 to 6 years. The questionnaires were distributed on social networking and others in a nursery school in Týn nad Vltavou. At the very beginning, respondents were acquainted with the topic of the thesis. The results of a quantitative survey were processed with the help of a data matrix and were further processed within twenty-arranged bar charts. To verify the hypothesis, we used the Chi- Quadrat test, which in this case is not satisfactory, therefore, there had to be chosen to merge the responses. An interrogation method, technique-depth interview was used in the qualitative part. The research group consisted of five nurses; three nurses working at GPCHA in Týn nad Vltavou and two working at GPCHA in České Budějovice. While processing the interviews, there open coding was used and an analysis of interviews was conducted by using the method of Pencil and Paper Interview. The results of this bachelor thesis will be published in the journal Paediatrics practice.
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The Use of the Addictive Drugs by the Seniors
KOPAČKOVÁ, Dominika
The bachelor thesis deals with using addictive substances by senior citizens. The age cathegoty 60+ should not be omitted when speaking of using addictive substances. Even senior citizens are users of some addictive substances, especially drugs. The thesis is divided into two parts, theoretical and explorative one. There are four chapters in the theoretical part (Old age and ageing, Substance addiction, Addictive substances and their classification, Usage of addictive substances in the old age) and summarizes the knowledge from recent literature about this topic. First, the old age and ageing are described. This chapter deals with ageing and old age of the human. This work describes the right mental hygiene, too. Multi-factorial manifestation of the old age (biological, psychological and social) is mentioned as well. In the second chapter there are described the factors that increase probability of the addiction origin. These factors can be genetic, biological, and psychological. There are mentioned some system models of the formation, development and maintenance of addiction, dependence and definition of the signs that addiction manifests. The third chapter divides addictive substances between legal and illegal. There are their effects, impacts on the human organism decribed. The group of legal addictive substances are included tobacco, alcohol, drugs and certain foods. Illegal substances represented here by cannabis, hallucinogens, stimulants, opiates. The last fourth chapter focuses directly on the addictive behaviour of the senior citizens. Seniors are also users of substances with addictive potential. At first alcoholism is mapped, other chapters devote addiction to nicotine, drugs and narcotics. The main goal of the thesis is to determine whether seniors use an addictive substance. A partial goal of is to find out the extent and cause of using addictive substances by the senior population. To find out these goals there were used the principles of quantitive research. To collect the data there were used questionnaires, created all by the author. The questionnaires were created with open and closed questiones and used in the printed form. The research has been conducted in Sušice and its close surroundings in February 2015. Respondents were the people with the least age limit of 70 years. These were 40 people living in their households or home for elderly. The results show that also amongst senior citizens can be found those ones who use the addictive substances. Current problem are by the doctor prescribed drugs. Today´s senior citizen uses drugs regularly, very often also combination of more drugs. Seniors often use medication to relieve the pain because the pain occurs more frequently. They use drugs to help them sleep better, too. The next group of addictive substances, which this chapter deals with, are drugs with a shoothing effect. There are smokers between the senior citizens who bare this bad habit from their midlife. Some research shows that cannabis helps people with Parkinson's and Alzheimer´s disease. Seniors do not use cannabis for these purposes because it is not legal. Alcohol is drunk by older people on occassion, on the other hand there are also people who drink such an amount that is not considered as not-risky. Seniors do not drink so much on one occasion, compared to the young generation. In the thesis this problem is described more detailed. Results of the research can be used by lay public to understand issues of addictive substances better. This thesis can be source of information for students of healt-social fields of study and it can improve the awareness about reasons of using addictive substances amongs senior citizens.
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